1887

Abstract

In order to analyse the expression of the non-structural (ns) protein p80/p125 in cells infected with different pestiviruses at the protein level, radioimmunoprecipitations with the pestivirus-specific monoclonal antibody (MAb) BVD/C16 were performed. Cell lysates infected with cytopathic (cp) and non-cytopathic (ncp) bovine viral diarrhoea (BVD) virus strains and isolates, and with hog cholera (HC) virus strains were analysed. From cpBVD virus-infected cells, the MAb precipitated one or more proteins corresponding to ns p125, displaying a marked size heterogeneity. In contrast, the lower ns p80 proteins from all cpBVD virus strains and isolates analysed had identical electrophoretic motility. The ncpBVD virus strains displayed either one single band or a doublet of the p125 protein and no p80 cleavage products. The p125 proteins precipitated from HC virus-infected cells showed no size heterogeneity. The possibility is discussed that multiple recombination events, including both insertions or deletions in the genomes of ncpBVD viruses, may lead to the heterogeneous expression of the ns p125 in cpBVD virus populations.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-1-47
1992-01-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/1/JV0730010047.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-1-47&mimeType=html&fmt=ahah

References

  1. Bolin S. R., McClurkin A. W., Cutlip R. C., Coria M. F. 1985; Severe clinical disease induced in cattle persistently infected with noncytopathic bovine viral diarrhea virus by superinfection with cytopathic bovine viral diarrhea virus. American Journal of Veterinary Research 46:573–576
    [Google Scholar]
  2. Brownlie J., Clarke M. C., Howard C. J. 1984; Experimental production of fatal mucosal disease in cattle. Veterinary Record 114:535–536
    [Google Scholar]
  3. Cay B., Chappuis G., Coulibaly C., Dinter Z., Edwards S., Greiser-Wilke I., Gunn M., Have P., Hess G., Junto N., L ess B., Mateo A., McHugh P., Moennig V., Nettleton P., Wensvoort G. 1989; Comparative analysis of monoclonal antibodies against pestiviruses: report of an international workshop. Veterinary Microbiology 20:123–129
    [Google Scholar]
  4. Collett M. S., Larson R., Belzer S. K., Retzel E. 1988a; Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology 165:200–208
    [Google Scholar]
  5. Collett M. S., Larson R., Gold C., Strick D., Anderson D. K., Purchio A. F. 1988b; Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology 165:191–199
    [Google Scholar]
  6. Collett M. S., Moennig V., Horzinek M. C. 1989; Recent advances in pestivirus research. Journal of General Virology 70:253–266
    [Google Scholar]
  7. Collett M. S., Wiskerchen M. A., Welniak E., Belzer S. K. 1991; Bovine viral diarrhea virus genomic organization. Archives of Virology supplement 3:19–27
    [Google Scholar]
  8. Desport M., Brownlie J. 1991; Molecular characterisation of the coding region for the p125 from homologous BVDV biotypes. Archives of Virology supplement 3:261–265
    [Google Scholar]
  9. Donis R. O., Dubovi E. J. 1987; Differences in virus-induced polypeptides in cells infected by cytopathic and noncytopathic biotypes of bovine virus diarrhea-mucosal disease virus. Virology 158:168–173
    [Google Scholar]
  10. Dutia B. M., Entrican G., Nettleton P. 1990; Cytopathic and non-cytopathic biotypes of border disease virus induce polypeptides of different molecular weight with common antigenic determinants. Journal of General Virology 71:1227–1232
    [Google Scholar]
  11. Gillespie J. H., Baker J. A., McEntee K. 1960; A cytopatho- genic strain of virus diarrhea virus. Cornell Veterinarian 50:73–79
    [Google Scholar]
  12. Greiser-Wilke I., Moennig V., Coulibaly C. O. Z., Dahle J., Leder L., Liess B. 1990; Identification of conserved epitopes on a hog cholera virus protein. Archives of Virology 111:213–225
    [Google Scholar]
  13. Greiser-Wilke I., Liess B., Schepers J., Stahl-Hennig C., Moennig V. 1991; Correlation of bovine viral diarrhoea virus induced cytopathic effects with expression of a biotype-specific marker. Archives of Virology supplement 3:55–65
    [Google Scholar]
  14. Holm-Jensen M. 1981; Detection of antibodies against hog cholera virus and bovine viral diarrhea virus in porcine serum. A comparative examination using CF, PLA and NPLA assays. Acta veterinaria scandinavica 22:85–98
    [Google Scholar]
  15. Horzinek M. C. 1991; Pestiviruses - taxonomic perspectives. Archives of Virology supplement 3:1–5
    [Google Scholar]
  16. Howard C. J., Brownlie J., Clarke M. C. 1987; Comparison by the neutralization assay of pairs of non-cytopathogenic and cytopathogenic strains of bovine virus diarrhoea virus isolated from cases of mucosal disease. Veterinary Microbiology 13:361–369
    [Google Scholar]
  17. Hyera J. M. K., Dahle J., Liess B., Moennig V., Frey H.-R. 1987; Production of potent antisera raised in pigs by anamnestic response and use for direct immunofluorescent and immunoperoxi- dase techniques. In Agriculture Pestivirus Infections of Ruminants pp 87–102 Edited by Harkness J. W. Brussels: Commission of the European Communities, publication CD-NA-10238-EN-C;
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  19. Lee K. M., Gillespie J. H. 1957; Propagation of virus diarrhea virus of cattle in tissue culture. American Journal of Veterinary Research 18:952–953
    [Google Scholar]
  20. McClurkin A. W., Bolin S. R., Coria M. F. 1985; Isolation of cytopathic and noncytopathic bovine viral diarrhea virus from the spleen of cattle acutely and chronically affected with bovine viral diarrhea. Journal of the American Veterinary Medical Association 186:568–569
    [Google Scholar]
  21. Magar R., Minocha H. C., Montpetit C., Carman P. S., Lecomte J. 1988; Typing of cytopathic and noncytopathic bovine viral diarrhea virus reference and Canadian field strains using a neutralizing monoclonal antibody. Canadian Journal of Veterinary Research 52:42–45
    [Google Scholar]
  22. Meyers G., Ruemenapf T., Thiel H. J. 1989; Ubiquitin in a togavirus. Nature, London 341:491
    [Google Scholar]
  23. Meyers G., Tautz N., Dubovi E. J., Thiel H.-J. 1991; Viral cytopathogenicity correlated with integration of ubiquitin-coding sequences. Virology 180:602–616
    [Google Scholar]
  24. Moennig V., Frey H.-R., Liebler E., Pohlenz J., Liess B. 1990; Reproduction of mucosal disease with cytopathogenic bovine viral diarrhoea virus selected in vitro. Veterinary Record 127:200–203
    [Google Scholar]
  25. Moormann R. J. M., Warmerdam P. A. M., Van Der Meer B., Schaaper W. M. M., Wensvoort G., Hulst M. M. 1990; Molecular cloning and nucleotide sequence of hog cholera virus strain Brescia and mapping of the genomic region encoding envelope protein El. Virology 177:184–198
    [Google Scholar]
  26. Peters W., Greiser-Wilke I., Moennig V., Liess B. 1986; Preliminary serological characterization of bovine viral diarrhoea virus strains using monoclonal antibodies. Veterinary Microbiology 12:195–200
    [Google Scholar]
  27. Pocock D. H., Howard C. J., Clarke M. C., Brownlie J. 1987; Variation in the intracellular polypeptide profiles from different isolates of bovine virus diarrhoea virus. Archives of Virology 94:43–53
    [Google Scholar]
  28. Purchio A. F., Larson R., Collett M. S. 1984; Characterization of bovine viral diarrhea virus proteins. Journal of Virology 50:666–669
    [Google Scholar]
  29. Ridpath J. F., Bolin S. R. 1990; Viral protein production in homogeneous and mixed infections of cytopathic and noncytopathic BVD virus. Archives of Virology 111:247–256
    [Google Scholar]
  30. Stark R., Ruemenapf T., Meyers G., Thiel H. J. 1990; Genomic localization of hog cholera virus glycoproteins. Virology 174:286–289
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-1-47
Loading
/content/journal/jgv/10.1099/0022-1317-73-1-47
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error