1887

Abstract

Antisera to a peptide representing the extreme carboxy terminus of the hepatitis delta virus antigen (HDAg) open reading frame (residues 197 to 211) recognized only the large (p27) and not the small (p24) form of HDAg in immunoblots of infected liver extracts, thereby providing direct proof that p27 and p24 differ in their carboxyl-terminal sequence and that p27 results from mutation within the stop codon terminating translation of p24. Reactions with other peptide antisera demonstrated that multiple smaller virus-specified proteins were carboxy-terminally truncated forms of HDAg, and immunoprecipitation studies suggested that different forms of HDAg were present as heterologous complexes within the liver extract.

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1992-01-01
2024-10-13
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References

  1. Bergmann K. F., Gerin J. L. 1986; Antigens of hepatitis delta virus in the liver and serum of humans and animals. Journal of Infectious Diseases 154:702–706
    [Google Scholar]
  2. Bergmann K. F., Cote P. J., Moriarty A., Gerin J. L. 1989; Hepatitis delta antigen: antigenic structure and humoral immune response. Journal of Immunology 143:3714–3721
    [Google Scholar]
  3. Bonino F., Heermann K. H., Rizzetto M., Gerlich W. H. 1986; Hepatitis delta virus: protein composition of delta antigen and its hepatitis B virus-derived envelope. Journal of Virology 58:945–950
    [Google Scholar]
  4. Chang M.-F., Baker S. C., Soe L. H., Kamahora T., Keck J. G., Makino S., Govindarajan S., Lai M. M. C. 1988; Human hepatitis delta antigen is a nuclear phosphoprotein with RNA-binding activity. Journal of Virology 62:2403–2410
    [Google Scholar]
  5. Chao M., Hsieh S.-Y., Taylor J. 1990; Role of two forms of hepatitis delta virus antigen: evidence for a mechanism of self-limiting genome replication. Journal of Virology 64:5066–5069
    [Google Scholar]
  6. Chao M., Hsieh S.-Y., Luo G., Taylor J. 1991; The antigen of human hepatitis delta virus: the significance of the two major electrophoretic forms. In The Hepatitis Delta Virus pp 275–281 Edited by Gerin L. J., Purcell R. H., Rizzetto M. New York: Wiley-Liss;
    [Google Scholar]
  7. Hsieh S.-Y., Chao M., Coates L., Taylor J. 1990; Hepatitis delta virus genome replication: a polyadenylated mRNA for delta antigen. Journal of Virology 64:3192–3198
    [Google Scholar]
  8. Jilbert A. R., Gowans E. J., Macnaughton T. B., Burrell C. B. 1991; Characterization of a subgenomic HDV-specific poly(A)+ RNA species isolated from human hepatoma cells supporting HDV RNA replication. In The Hepatitis Delta Virus pp 309–314 Edited by Gerin L. J., Purcell R. H., Rizzetto M. New York: Wiley-Liss;
    [Google Scholar]
  9. Kuo M. Y. P., Goldberg J. D., Coates L., Mason W. S., Gerin J. L., Taylor J. 1988; Molecular cloning of hepatitis delta virus RNA from an infected woodchuck liver: sequence, structure, and applications. Journal of Virology 62:1855–1861
    [Google Scholar]
  10. Lai M. M. C., Chao Y.-C., Chang M.-F., Lin J.-H., Gust I. 1991; Functional studies of hepatitis delta antigen and delta virus RNA. In The Hepatitis Delta Virus pp 283–292 Edited by Gerin L. J. Purcell R. H., Rizzetto M. New York: Wiley-Liss;
    [Google Scholar]
  11. Luo G., Chao M., Hsieh S.-Y., Sureau C., Nishikura K., Taylor J. 1990; A specific base transition occurs on replicating hepatitis delta virus RNA. Journal of Virology 64:1021–1027
    [Google Scholar]
  12. Makino S., Chang M.-F., Shieh C.-K., Kamahora T., Vannier D. M., Govindarajan S., Lai M. M. C. 1987; Molecular cloning and sequencing of a human hepatitis delta (δ) virus RNA. Nature, London 329:343–346
    [Google Scholar]
  13. Negro F., Korba B. E., Forzani B., Baroudy B. M., Brown T. L., Gerin J. L., Ponzetto A. 1989; Hepatitis delta virus (HDV) and woodchuck hepatitis virus (WHV) nucleic acids in tissues of HDV-infected chronic WHV carrier woodchucks. Journal of Virology 63:1612–1618
    [Google Scholar]
  14. Ou J.-H., Yeh C.-T., Yen T. S. B. 1989; Transport of hepatitis B virus precore protein into the nucleus after cleavage of its signal peptide. Journal of Virology 63:5238–5243
    [Google Scholar]
  15. Rizzetto M., Verme G. 1985; Delta hepatitis: present status. Journal of Hepatology 1:187
    [Google Scholar]
  16. Rizzetto M., Hoyer B., Canese M. G., Shih J. W.-K., Purcell R. H., Gerin J. L. 1980; δ agent: association of δ antigen with hepatitis B surface antigen and RNA in serum of δ-infected chimpanzees. Proceedings of the National Academy of Sciences, U.S.A. 77:6124–6128
    [Google Scholar]
  17. Taylor J. M. 1990; Hepatitis delta virus: cis and trans functions required for replication. Cell 61:371–373
    [Google Scholar]
  18. Wang K.-S., Choo Q.-L., Weiner A. J., Ou J.-H., Najarian R. C., Thayer R. M., Mullenbach G. T., Denniston K. J., Gerin J. L., Houghton M. 1986; Structure, sequence and expression of the hepatitis delta (δ) viral genome. Nature, London 323:508–514
    [Google Scholar]
  19. Wang K.-S., Choo Q.-L., Weiner A. J., Ou J.-H., Najarian R. C., Thayer R. M., Mullenbach G. T., Denniston K. J., Gerin J. L., Houghton M. 1987; Corrigendum: structure, sequence and expression of the hepatitis delta (δ) viral genome. Nature, London 328:456
    [Google Scholar]
  20. Wang J. G., Jansen R. W., Brown E. A., Lemon S. M. 1990; Immunogenic domains of hepatitis delta virus antigen: peptide mapping of epitopes recognized by human and woodchuck antibodies. Journal of Virology 64:1108–1116
    [Google Scholar]
  21. Weiner A. J., Choo Q.-L., Wang K.-S., Govindarajan S., Redeker A. G., Gerin J. L., Houghton M. 1988; A single antigenomic open reading frame of the hepatitis delta virus encodes the epitope(δ) of both hepatitis delta antigen polypeptides p24 δ and P27 δ . Journal of Virology 62:594–599
    [Google Scholar]
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