1887

Abstract

The nucleotide sequences of cDNAs of the simian virus 5 (SV5) nucleoprotein (NP) gene, and the 3′ end of the genome and NP gene of SV41 were determined. The open reading frames of the SV5 and SV41 NP genes encode polypeptides with s of 56582 and 60575, respectively, values which are consistent with those estimated by SDS-PAGE. The NP of human parainfluenza virus type 2 (hPIV-2) was more closely related to that of SV41 (amino acid sequence identity 70.5%) than that of SV5 (57.0%); the amino acid sequence identity between the NPs of SV41 and SV5 was 63.3%. The sequence of the 3′ end of the genome of SV41 showed a high level of similarity to that of hPIV-2, the terminal 18 nucleotides being identical. It is concluded from these findings that SV41 is related most closely to hPIV-2, even though SV5 had been thought to be an animal type of hPIV-2.

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1991-09-01
2022-10-03
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References

  1. Barr J., Chambers P., Pringle C. R., Easton A. J. 1991; Sequence of the major nucleocapsid protein gene of pneumonia virus of mice: sequence comparisons suggest structural homology between nucleocapsid proteins of pneumoviruses, paramyxoviruses, rhabdoid-viruses and filoviruses. Journal of General Virology 72:677–685
    [Google Scholar]
  2. Chanock L. J., McIntosh K. 1985; Parainfluenza viruses. In Virology pp 1241–1253 Edited by Fields B. W. New York: Raven Press;
    [Google Scholar]
  3. Goswami K. K. A., Russell W. C. 1982; A comparison of paramyxoviruses by immunoprecipitation. Journal of General Virology 60:177–183
    [Google Scholar]
  4. Goswami K. K. A., Lange L. S., Mitchell D. N., Cameron K. R., Russell W. C. 1984; Does simian virus 5 infect humans?. Journal of General Virology 65:1295–1303
    [Google Scholar]
  5. Goswami K. K. A., Randall R. E., Lange L. S., Russell W. C. 1987; Antibodies against the paramyxovirus SV5 in the cerebro-spinal fluids of some multiple sclerosis patients. Nature, London 327:244–247
    [Google Scholar]
  6. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  7. Holmes D. C., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193
    [Google Scholar]
  8. Hull R. N., Minner J. R., Mascoli C. C. 1958; New viral agents recovered from tissue cultures of monkey kidney cells. III. Recovery of additional agents both from cultures of monkey tissues and directly from tissues and excreta. American Journal of Hygiene 68:31–44
    [Google Scholar]
  9. Inokuchi Y., Hirashima A., Watanabe I. 1982; Comparison of the nucleotide sequences at the 3′-terminal region of RNAs from RNA coliphages. Journal of Molecular Biology 158:711–730
    [Google Scholar]
  10. Ito Y., Tsurudome M., Hishiyama M., Yamada A. 1987; Immunological interrelationships among human and non-human paramyxoviruses revealed by immunoprecipitation. Journal of General Virology 68:1289–1297
    [Google Scholar]
  11. Kawano M., Bando H., Yuasa T., Kondo K., Tsurudome M., Komada H., Nishio M., Ito Y. 1990; Sequence determination of the hemagglutinin-neuraminidase (HN) gene of human parainfluenza type 2 virus and construction of a phylogenetic tree for HN proteins of all the paramyxoviruses that are infectious to humans. Virology 174:308–313
    [Google Scholar]
  12. Kingsbury D. W., Bratt M. A., Choppin P. W., Hanson R. P., Hosaka Y., ter Meulen V., Norrby E., Plowright W., Rott R., Wunner W. H. 1978; Paramyxoviridae. Intervirology 10:137–152
    [Google Scholar]
  13. Komada H., Tsurudome H. M., Ueda M., Nishio M., Bando H., Ito Y. 1989; Isolation and characterization of monoclonal antibodies to human parainfluenza virus type 4 and their use in revealing the antigenic relationship between subtypes 4A and 4B. Virology 171:28–37
    [Google Scholar]
  14. Kondo K., Bando H., Kawano M., Tsurudome M., Komada H., Nishio M., Ito Y. 1990; Sequencing analyses and comparison of parainfluenza virus type 4A and 4B NP protein genes. Virology 174:1–8
    [Google Scholar]
  15. Lyn D., Gill D. S., Scroggs R. A., Portner A. 1991; The nucleoproteins of human parainfluenza virus type 1 and Sendai virus share amino acid sequences and antigenic and structural determinants. Journal of General Virology 72:983–987
    [Google Scholar]
  16. Matsuoka Y., Ray R. 1991; Sequence analysis and expression of the human parainfluenza type 1 virus nucleoprotein gene. Virology 181:403–407
    [Google Scholar]
  17. Miller R. H., Pursell A. R., Mitchell F. E., Johnson K. M. 1964; A newly discovered myxovirus (S.V.41) isolated from cell cultures of cynomolgus monkey kidney. American Journal of Hygiene 80:365–376
    [Google Scholar]
  18. Nishio M., Tsurudome M., Bando H., Ito Y. 1990; Immunological relationships of simian virus 41 (SV41) to other paramyxoviruses and serological evidence of SV41 infection in human populations. Journal of General Virology 71:2093–2097
    [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  20. Tsurudome M., Nishio M., Komada H., Bando H., Ito Y. 1989; Extensive antigenic diversity among human parainfluenza type 2 virus isolates and immunological relationships among paramyxoviruses revealed by monoclonal antibodies. Virology 171:38–48
    [Google Scholar]
  21. Tsurudome M., Bando H., Nishio M., Iwamoto Y., Kawano M., Kondo K., Komada H., Ito Y. 1991; Antigenic and structural properties of a paramyxovirus simian virus 41 (SV41) reveal a close relationship with human parainfluenza type 2 virus. Virology 179:738–748
    [Google Scholar]
  22. Yuasa T., Bando H., Kawano M., Tsurudome M., Nishio M., Komada H., Ito Y. 1990; Sequence analyses of the 3′ genome end and NP gene of human parainfluenza type 2 virus: sequence variation of the gene-starting signal and the conserved 3′ end. Virology 179:777–784
    [Google Scholar]
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