1887

Abstract

The thermal denaturation of poliovirus virions and procapsids in the 42 to 48 °C range was studied using N- and H-specific monoclonal antibodies. The half-life of the N antigen of Mahoney and Sabin 1 virions was extended 50- to 250-fold by either 10 µ WIN 51711 or R 78206. The minimum concentrations required for full stabilization at 46 °C (1.0 µ for WIN 51711, 0.2 µ for R 78206) were independent of the strain or serotype of the virus; 30 to 60 molecules of stabilizer per virion were required for full protection. R 78206 was the most efficient stabilizer of Mahoney procapsids; the half-life of the N-specific epitopes of these particles at 44 °C was extended from less than 1 min to 1 day.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-9-2153
1991-09-01
2019-11-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-9-2153
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error