1887

Abstract

The haemagglutinins (HAs) of five H3 influenza A viruses isolated from domestic ducks and one from a goose in southern China were analysed antigenically and genetically. The patterns of reactivity of two of the duck viruses and the goose virus with a panel of monoclonal antibodies to 10 different epitopes on the H3 HA were similar to those of influenza viruses isolated from wild ducks and pigs, as well as those of the earliest human H3 viruses. The other three isolates from domestic ducks were different from each other and from these viruses antigenically. Sequence analysis revealed that the HA genes of the two duck viruses and the goose virus were closely related to those of isolates from wild ducks and pigs; the identities between the deduced amino acid sequence of the HA of one of the isolates from domestic ducks and those of isolates from a wild duck and a pig were 98.7% and 99.5%, respectively. The antigenic and genetic similarity between these H3 HAs suggests that in southern China, the hypothetical influenza epicentre, domestic ducks may have played a role in the introduction of avian influenza viruses to pigs from feral ducks. The findings also support the hypothesis that the pig was a ‘mixing vessel’, producing a new human pandemic strain, A/Hong Kong/68 (H3N2), by genetic reassortment.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-8-2007
1991-08-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/8/JV0720082007.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-8-2007&mimeType=html&fmt=ahah

References

  1. Bean W. J., Sriram G., Webster R. G. 1980; Electrophoretic analysis of iodine-labelled influenza virus RNA segments. Analytical Biochemistry 102:228–232
    [Google Scholar]
  2. Biggin M. D., Gibson T. J., Hong G. F. 1983; Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences, U.S.A. 80:3963–3965
    [Google Scholar]
  3. Kida H., Yanagawa R. 1979; Isolation of a new avian paramyxovirus from a rock pigeon (Columba livia). Zentralblatt fůr Bakteriologie, Mikrobiologie und Hygiene A245:421–428
    [Google Scholar]
  4. Kida H., Brown L. E., Webster R. G. 1982; Biological activity of monoclonal antibodies to operationally defined antigenic regions on the hemagglutinin molecule of A/seal/Massachusetts/l/80(H7N7) influenza virus. Virology 122:38–47
    [Google Scholar]
  5. Kida H., Kawaoka Y., Naeve C. W., Webster R. G. 1987; Antigenic and genetic conservation of H3 influenza virus in wild ducks. Virology 159:109–119
    [Google Scholar]
  6. Kida H., Shortridge K. F., Webster R. G. 1988; Origin of the hemagglutinin gene of H3N2 influenza viruses from pigs in China. Virology 162:160–166
    [Google Scholar]
  7. Kida H., Webster R. G., Kawaoka Y., Shortridge K. F. Naeve C. W., Ito T., Itakura C., Mori F, Shimizu Y. 1989; Origin of the hemagglutinin gene of A/Hong Kong/68 (H3N2) influenza virus. In Current Topics in Medical Virology pp. 365–376 Edited by Chan Y. C., Doraisingham S., Ling A. E. Singapore & London: World Scientific;
    [Google Scholar]
  8. Rogers G. N., Paulson J. C., Daniels R. S., Skehel J. J., Wilson I. A., Wiley D. C. 1983; Single amino acid substitutions in influenza haemagglutinin change receptor binding specificity. Nature, London 304:76–78
    [Google Scholar]
  9. Sanger F. S., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  10. Scholtissek C., Rohde W., Von Hoyningen V., Rott R. 1978; On the origin of the human influenza virus subtypes H2N2 and H3N2. Virology 87:13–20
    [Google Scholar]
  11. Scholtissek C., Burger H., Kistner O., Shortridge K. F. 1985; The nucleoprotein as a possible major factor in determining host specificity of influenza H3N2 viruses. Virology 147:287–294
    [Google Scholar]
  12. Shortridge K. F. 1981; Epidemiology of avian influenza and sources of infection in domestic species. In Proceedings of the First International Symposium on Avian Influenza, Beltsville, Md pp 5468 Edited by Bankowski R. A. Richmond: Carter Composition;
    [Google Scholar]
  13. Shortridge K. F. 1982; Avian influenza A viruses of southern China and Hong Kong: ecological aspects and implications for man. Bulletin of the World Health Organization 60:129–135
    [Google Scholar]
  14. Shortridge K. F., Stuart-Harris C. H. 1982; An influenza epicentre?. Lancet ii:812–813
    [Google Scholar]
  15. Shortridge K. F., Butterfield W. K., Webster R. G., Campbell C. H. 1977; Isolation and characterization of influenza A viruses from avian species in Hong Kong. Bulletin of the World Health Organization 55:15–20
    [Google Scholar]
  16. Shortridge K. F., Butterfield W.-K., Webster R. G., Campbell C. H. 1979; Diversity of influenza A virus subtypes isolated from domestic poultry in Hong Kong. Bulletin of the World Health Organization 57:465–469
    [Google Scholar]
  17. Verhoeyen M., Fang R., Min Jou W., Devos R., Huylebroeck D., Saman E., Fiers W. 1980; Antigenic drift between the haemagglutinin of the Hong Kong influenza strains A/Aichi/2/68 and A/Victoria/3/75. Nature, London 286:771–776
    [Google Scholar]
  18. Webster R. G., Laver W. G. 1967; Preparation and properties of antibody directed specifically against the neuraminidase of an influenza virus. Journal of Immunology 99:49–55
    [Google Scholar]
  19. Wilson I. A., Skehel J. J., Wiley D. C. 1981; Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution. Nature, London 289:366–373
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-8-2007
Loading
/content/journal/jgv/10.1099/0022-1317-72-8-2007
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error