1887

Abstract

Newcastle disease virus (NDV) virions possess two proteins which react strongly with monoclonal antibody 688 following separation by high resolution two-dimensional (isoelectric focusing/SDS) PAGE and detection by Western blotting. One is the phosphorylated nucleocapsid-associated 53K [P (NAP)] protein, the other comigrates with the 36K protein detected by radiolabelling NDV-infected chick embryo fibroblasts. [S]Cysteine/[H]leucine dual-labelling experiments show that the 36K protein is very rich in cysteine compared to the P (NAP) protein. In the Beaudette C strain it comigrates on one-dimensional SDS-polyacrylamide gels with the matrix protein (M); however, it is resolved from the slower migrating M protein from the Ulster strain of NDV. The size, strain-specific isoelectric point, high cysteine content and antigenic relatedness to the P (NAP) protein suggest that the 36K protein is the ‘V’ protein of NDV, the counterpart of which is found in other .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-7-1709
1991-07-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/7/JV0720071709.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-7-1709&mimeType=html&fmt=ahah

References

  1. Anker H. S. 1970; A solubilizable acrylamide gel for electrophoresis. FEBS Letters 7:293
    [Google Scholar]
  2. Barrett T., Shrimpton S. B., Russell S. E. H. 1985; Nucleotide sequence of the entire protein coding region of canine distemper virus polymerase-associated (P) protein mRNA. Virus Research 3:367–372
    [Google Scholar]
  3. Bellini W. J., Englund G., Rozenblatt S., Arnheiter H., Richardson C. D. 1985; Measles virus P gene codes for two proteins. Journal of Virology 53:908–919
    [Google Scholar]
  4. Cattaneo R., Kaelin K., Baczko K., Billeter M. A. 1989; Measles virus editing provides an additional cysteine-rich protein. Cell 56:759–764
    [Google Scholar]
  5. Chambers P., Samson A. C. R. 1980; A new structural protein for Newcastle disease virus. Journal of General Virology 50:155–166
    [Google Scholar]
  6. Chambers P., Samson A. C. R. 1982; Non-structural proteins in Newcastle disease virus-infected cells. Journal of General Virology 58:1–12
    [Google Scholar]
  7. Choppin P. W., Compans R. 1975; Reproduction of paramyxoviruses. In Comprehensive Virology vol. 4 pp. 95–178 Edited by Fraenkel-Conrat H., Wagner R. R. New York & London: Plenum Press;
    [Google Scholar]
  8. Collins P. L., Wertz G. W., Ball L. A., Hightower L. E. 1982; Coding assignments of the five smaller mRNAs of Newcastle disease virus. Journal of Virology 43:1024–1031
    [Google Scholar]
  9. Curran J. A., Richardson C. D., Kolakofsky D. 1986; Ribosomal initiation of alternate AUGs on the Sendai virus P/C mRNA. Journal of Virology 57:684–687
    [Google Scholar]
  10. Elliot G. D., Yeo R. P., Afzal M. A., Simpson E. J. B., Curran J. A., Rima B. K. 1990; Strain-variable editing during transcription of the P gene of mumps virus may lead to the generation of non-structural proteins NS1 (V) and NS2. Journal of General Virology 71:1555–1560
    [Google Scholar]
  11. Galinski M. S., Mink M. A., Lambert D. M., Wechsler S. L., Pons M. W. 1986; Molecular cloning and sequence analysis of the human parainfluenza 3 virus mRNA encoding the P and C proteins. Virology 155:46–60
    [Google Scholar]
  12. Giorgi C., Blumberg B. M., Kolakofsky D. 1983; Sendai virus contains overlapping genes expressed from a single mRNA. Cell 35:829–836
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Luk D., Sanchez A., Banerjee A. 1986; Messenger RNA encoding the phosphoprotein (P) gene of human parainfluenza virus 3 is bicistronic. Virology 153:318–325
    [Google Scholar]
  15. McGinnes L., McQuajn C., Morrison T. 1988; The P protein and the non-structural 38K and 29K proteins of Newcastle disease virus are derived from the same open reading frame. Virology 164:256–264
    [Google Scholar]
  16. Millar N. S., Chambers P., Emmerson P. T. 1988; Nucleotide sequence of the fusion and haemagglutinin-neuraminidase glycoprotein genes of Newcastle disease virus, strain Ulster: molecular basis for variations in pathogenicity between strains. Journal of General Virology 69:613–620
    [Google Scholar]
  17. O’Farrell P. H. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  18. Paterson R. G., Lamb R. A. 1990; RNA editing by G-nucleotide insertion in mumps virus P-gene mRNA transcripts. Journal of Virology 64:4137–4145
    [Google Scholar]
  19. Paterson R. G., Thomas S. M., Lamb R. A. 1989; Specific nontemplated nucleotide addition to a simian virus 5 mRNA: prediction of a common mechanism by which unrecognized hybrid P-cysteine-rich proteins are encoded by paramyxovirus ‘P’ genes. In Genetics and Pathogenicity of Negative Strand Viruses pp. 232–245 Edited by Kolaicofsky D., Mahy B. W. J. Amsterdam: Elsevier;
    [Google Scholar]
  20. Russell P. H., Griffiths P. C., Goswami K. K. A., Alexander D. J., Cannon M. J., Russell W. C. 1983; The characterization of monoclonal antibodies to Newcastle disease virus. Journal of General Virology 64:2069–2072
    [Google Scholar]
  21. Samson A. C. R. 1986; Anomalous behaviour of Newcastle disease virus haemagglutinin-neuraminidase protein in Western blotting analysis of monoclonal antibody binding sites. Journal of General Virology 67:1199–1203
    [Google Scholar]
  22. Samson A. C. R. 1988; Virus structure. In Newcastle Disease pp. 23–44 Edited by Alexander D. J. Boston: Kluwer;
    [Google Scholar]
  23. Samson A. C. R., Chambers P., Lee C. M., Simon E. 1981; Temperature-sensitive mutant of Newcastle disease virus which has an altered nucleocapsid-associated protein. Journal of General Virology 54:197–201
    [Google Scholar]
  24. Sato H., Oh-Hira M., Ishida N., Imamura Y., Hattori S., Kawakita M. 1987; Molecular cloning and nucleotide sequence of P, M and F genes of Newcastle disease virus avirulent strain D26. Virus Research 7:241–255
    [Google Scholar]
  25. Simpson E. J. B., Curran J. A., Martin S. J., Hoey E. M., Rima B. K. 1984; Characterization of mumps virus proteins and RNA. In The Molecular Biology of Negative Strand Viruses pp. 333–338 Edited by Bishop D. H. L., Compans R. W. New York: Academic Press;
    [Google Scholar]
  26. Southern J. A., Precious B., Randall R. E. 1990; Two nontemplated nucleotide additions are required to generate the P mRNA of parainfluenza virus type 2 since the RNA genome encodes protein V. Virology 177:388–390
    [Google Scholar]
  27. Spriggs M. K., Collins P. L. 1986; Sequence analysis of the P and C protein genes of human parainfluenza virus type 3: patterns of amino acid sequence homology among paramyxovirus proteins. Journal of General Virology 67:2705–2719
    [Google Scholar]
  28. Takeuchi K., Hishiyama M., Yamada A., Sugiura A. 1988; Molecular cloning and sequence analysis of the mumps virus gene encoding the P protein: mumps virus P gene is monocistronic. Journal of General Virology 69:2043–2049
    [Google Scholar]
  29. Takeuchi K., Tanabayashi K., Hishiyama M., Yamada K. K., Yamada A., Sugiura A. 1990; Detection and characterization of mumps virus V protein. Virology 178:247–253
    [Google Scholar]
  30. Thomas S. M., Lamb R. A., Paterson R. G. 1988; Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5. Cell 54:891–902
    [Google Scholar]
  31. Vidal S., Curran J., Kolakofsky D. 1990; Editing of the Sendai virus P/C mRNA by G insertion occurs during mRNA synthesis via a virus-encoded activity. Journal of Virology 64:239–246
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-7-1709
Loading
/content/journal/jgv/10.1099/0022-1317-72-7-1709
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error