1887

Abstract

The nucleotide sequences of the coding region of the thymidine kinase (TK) gene of pseudorabies virus strain NIA3 and a TK mutant (ATK5) were determined. The coding region of the TK gene consists of 320 codons capable of producing a polypeptide with an of 34979. The mutant expressed an inactive TK polypeptide when translated ; a unique base substitution was detected in the mutant TK modifying amino acid position 13, at which aspartic acid replaces glycine. This modification affects the nucleotide-binding site, thus explaining the expression of an inactive TK polypeptide.

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1991-06-01
2022-09-25
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References

  1. Bajszar G., Witter R., Weir J., Moss B. 1983; Vaccinia virus thymidine kinase and neighboring genes: mRNA and polypeptides of wild-type virus and putative nonsense mutants. Journal of Virology 45:62–72
    [Google Scholar]
  2. Baskerville A., McFerran J. B., Dow C. 1973; Aujeszky’s disease in pigs. Veterinary Bulletin 43:465–480
    [Google Scholar]
  3. Ben-Porat T., Kaplan A. S. 1985; Molecular biology of pseudorabies virus. In The Herpesviruses vol 3 pp 105–173 Edited by Roizman B. New York: Plenum;
    [Google Scholar]
  4. Ben-Porat T., Veach R. A., Ihara S. 1983; Localization of the regions of homology between the genomes of herpes simplex virus type 1 and pseudorabies virus. Virology 127:194–204
    [Google Scholar]
  5. Boyle D. B., Coupar B. E. H., Gibbs A. J., Seigman L. J., Both G. W. 1987; Fowlpox virus thymidine kinase: nucleotide sequence and relationships to other thymidine kinases. Virology 156:355–365
    [Google Scholar]
  6. Bujard H., Gentz R., Lanzer M., Stueber D., Mueller M., Ibrahimi I., Haeuptle M. T., Dobberstein B. 1987; A T5 promoter-based transcription-translation system for the analysis of proteins in vitro and in vivo . Methods in Enzymology 155:416–433
    [Google Scholar]
  7. Darby G., Larder B. A., Inglis M. M. 1986; Evidence that the ‘active centre’ of the herpes simplex virus thymidine kinase involves an interaction between three distinct regions of the polypeptide. Journal of General Virology 67:753–758
    [Google Scholar]
  8. Davison A. J., Scott J. E. 1986; The complete DNA sequence of varicella-zoster virus. Journal of General Virology 67:1759–1816
    [Google Scholar]
  9. Efstathiou S., Kemp S., Darby G., Minson A. C. 1989; The role of herpes simplex virus type 1 thymidine kinase in pathogenesis. Journal of General Virology 70:869–879
    [Google Scholar]
  10. Gentry G. A. 1985; Locating a nucleotide-binding site in the thymidine kinase of vaccinia virus and of herpes simplex virus by scoring triply aligned protein sequences. Proceedings of the National Academy of Sciences, U.S.A 82:6815–6819
    [Google Scholar]
  11. Gielkens A. L. J., Van Oirschot J. T., Berns A. J. M. 1985; Genome differences among field isolates and vaccine strains of pseudorabies virus. Journal of General Virology 66:69–82
    [Google Scholar]
  12. Hiraga S., Igarashi K., Yura T. 1967; Deoxythymidine kinase-deficient mutant of Escherichia coli. I. Isolation and some properties. Biochimica et biophysica acta 145:41–51
    [Google Scholar]
  13. Honess R. W., Craxton M. A., Williams L., Gompels U. A. 1989; A comparative analysis of the sequence of the thymidine kinase gene of a gammaherpesvirus, herpesvirus saimiri. Journal of General Virology 70:3003–3013
    [Google Scholar]
  14. Kit S. 1985a U. S. Patent Application no. 4514497
    [Google Scholar]
  15. Kit S. 1985b; Thymidine kinase. Microbiological Sciences 2:369–375
    [Google Scholar]
  16. Kit S., Kit M., Pirtle E. C. 1985; Attenuated properties of thymidine kinase-negative deletion mutant of pseudorabies virus. American Journal of Veterinary Research 46:1359–1367
    [Google Scholar]
  17. Liu Q., Summers W. C. 1988; Site-directed mutagenesis of a nucleotide-binding domain in HSV-1 thymidine kinase: effects on catalytic activity. Virology 163:638–642
    [Google Scholar]
  18. McGregor S., Easterdav B. C., Kaplan A. S., Ben-Porat T. 1985; Vaccination of swine with thymidine kinase-deficient mutants of pseudorabies virus. American Journal of Veterinary Research 46:1494–1497
    [Google Scholar]
  19. McKnight S. L. 1980; The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene. Nucleic Acids Research 8:5949–5964
    [Google Scholar]
  20. McKnight S. L., Kingsbury R. C., Spence A., Smith M. 1984; The distal transcription signals of the herpesvirus tk gene share a common hexanucleotide control sequence. Cell 37:253–262
    [Google Scholar]
  21. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Mittal S. K., Field H. J. 1989; Analysis of the bovine herpesvirus type 1 thymidine kinase (TK) gene from wild-type and TK-deficient mutants. Journal of General Virology 70:901–918
    [Google Scholar]
  23. Moormann R. J. M., De Rover T., Briaire J., Peeters B. P. H., Gielkens A. L. J., Van Oirschot J. T. 1990; Inactivation of the thymidine kinase gene of a gl deletion mutant of pseudorabies virus generates a safe but still highly immunogenic vaccine strain. Journal of General Virology 71:1591–1595
    [Google Scholar]
  24. Nunberg J. H., Wright D. K., Cole G. E., Petrovskis E. A., Post L. E., Compton T., Gilbert J. H. 1989; Identification of the thymidine kinase gene of feline herpesvirus: use of degenerate oligonucleotides in the polymerase chain reaction to isolate herpesvirus gene homologs. Journal of Virology 63:3240–3249
    [Google Scholar]
  25. Otsuka H., Kit S. 1984; Nucleotide sequence of the marmoset herpesvirus thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide. Virology 135:316–330
    [Google Scholar]
  26. Proudfoot N. J., Brownlee G. G. 1976; 3′ non-coding region sequences in eukaryotic messenger RNA. Nature, London 263:211–214
    [Google Scholar]
  27. Robertson G. R., Whalley J. M. 1988; Evolution of the herpes thymidine kinase: identification and comparison of the equine herpesvirus 1 thymidine kinase gene reveals similarity to a cell-encoded thymidylate kinase. Nucleic Acids Research 16:11303–11317
    [Google Scholar]
  28. Roizman B., Jenkins F. J. 1985; Genetic engineering of novel genomes of large DNA viruses. Science 229:1208–1214
    [Google Scholar]
  29. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  30. Scott S. D., Ross N. L. J., Binns M. M. 1989; Nucleotide and predicted amino acid sequences of the Marek’s disease virus and turkey herpesvirus thymidine kinase genes; comparison with thymidine kinase genes of other herpesviruses. Journal of General Virology 70:3055–3065
    [Google Scholar]
  31. Sheppard M., May J. T. 1989; Location and characterization of the bovine herpesvirus type 2 thymidine kinase gene. Journal of General Virology 70:3067–3071
    [Google Scholar]
  32. Swain M. A., Galloway D. A. 1983; Nucleotide sequence of the herpes simplex virus type 2 thymidine kinase gene. Journal of Virology 46:1045–1050
    [Google Scholar]
  33. Tabarés E., Martinez J., Gonzalvo F. R., Sanchez Botija C. 1980b; Proteins specified by African swine fever virus. II. Analysis of proteins in infected cells and antigenic properties. Archives of Virology 66:119–132
    [Google Scholar]
  34. Tabarés E., Martinez J., Gonzalvo F. R., Sanchez Botija C. 1980b; Proteins specified by African swine fever virus. II. Analysis of proteins in infected cells and antigenic properties. Archives of Virology 66:119–132
    [Google Scholar]
  35. Tabarés E., Olivares I., Santurde G., García M. J., Martin E., Carnero M. E. 1987; African swine fever virus DNA: deletions and additions during adaptation to growth in monkey kidney cells. Archives of Virology 97:333–346
    [Google Scholar]
  36. Tenser R. B., Ressel S. J., Fralish F. A., Jones J. C. 1983; The role of pseudorabies virus thymidine kinase expression in trigeminal ganglion infection. Journal of General Virology 64:1369–1373
    [Google Scholar]
  37. Thomsen D. R., Marotti K. R., Palermo D. P., Post L. E. 1987; Pseudorabies virus as a live virus vector for expression of foreign genes. Gene 57:261–265
    [Google Scholar]
  38. Wagner M. J., Sharp J. A., Summers W. C. 1981; Nucleotide sequence of thymidine kinase gene of herpes simplex virus type 1. Proceedings of the National Academy of Sciences, U.S.A 78:1441–1445
    [Google Scholar]
  39. Weir J. P., Moss B. 1983; Nucleotide sequence of the vaccinia virus thymidine kinase gene and the nature of spontaneous frameshift mutations. Journal of Virology 46:530–537
    [Google Scholar]
  40. Weir J. P., Bajszar G., Moss B. 1982; Mapping of the vaccinia virus thymidine kinase gene by marker rescue and by cell-free translation of selected mRNA. Proceedings of the National Academy of Sciences, U.S.A 79:1210–1214
    [Google Scholar]
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