1887

Abstract

We have investigated the correlation between restriction fragment length polymorphisms of the scrapie-associated fibril protein (PrP) gene and the incidence of natural scrapie in British sheep during the period from July 1988 to November 1990. Sixty percent of the scrapie-positive animals studied were homozygous for a 6.8 kb RI fragment (e1) and a further 26% carried e1 as heterozygotes. This fragment is linked to susceptibility to experimental scrapie in a closed flock of Cheviot sheep. Twelve percent of cases were found to be homozygous for a 4.4 kb RI fragment (e3) which in the Cheviot flock has been linked to relative resistance to scrapie. A third RI fragment of 5.2 kb (e2) has also been found but is relatively rare and has not yet been associated with scrapie susceptibility. Four sets of flocks affected by natural outbreaks of scrapie divided into two groups. In three of these flocks, all scrapie cases carried e1 with high frequencies of e1e1 homozygotes. In the fourth, there were no e1e1 scrapie cases; all scrapie sheep carried e3 in approximately equal numbers of heterozygotes and homozygotes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-6-1287
1991-06-01
2024-12-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/6/JV0720061287.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-6-1287&mimeType=html&fmt=ahah

References

  1. Carlson G. A., Goodman P. A., Lovett M., Taylor B. A., Marshall S. T., Petersa-Torchia M., Westaway D., Prusiner S. B. 1989; Genetics and polymorphism of the mouse prion gene complex: control of scrapie incubation. Molecular and Cellular Biology 8:5528–5540
    [Google Scholar]
  2. Dickinson A. G. 1976; Scrapie in sheep and goats. In Slow Virus Diseases of Animals and Man pp 209–242 Edited by Kimberlin R. H. Amsterdam: North-Holland;
    [Google Scholar]
  3. Dickinson A. G., Outram G. W. 1988; Genetic aspects of unconventional virus infections: the basis of the virino hypothesis. In Novel Infectious Agents and the Central Nervous System Ciba Foundation Symposium No 135 pp 63–83 Edited by Bock G., Marsh J. Chichester: Wiley;
    [Google Scholar]
  4. Dickinson A. G., Young G. B., Stamp J. T., Renwick C. C. 1964; A note on the distribution of scrapie in sheep of different ages. Animal Production 6:375–377
    [Google Scholar]
  5. Dickinson A. G., Young G. B., Stamp J. T., Renwick C. C. 1965; An analysis of natural scrapie in Suffolk sheep. Heredity 20:485–503
    [Google Scholar]
  6. Dickinson A. G., Stamp J. T., Renwick C. C., Rennie J. C. 1968; Some factors controlling the incidence of scrapie in Cheviot sheep injected with a Cheviot-passaged scrapie agent. Journal of Comparative Pathology 78:313–321
    [Google Scholar]
  7. Dickinson A. G., Stamp J. T., Renwick C. C. 1974; Maternal and lateral transmission of scrapie in sheep. Journal of Comparative Pathology 84:19–25
    [Google Scholar]
  8. Foster J. D., Dickinson A. G. 1988a; The unusual properties of CH1641, a sheep-passaged isolate of scrapie. Veterinary Record 123:5–8
    [Google Scholar]
  9. Foster J. D., Dickinson A. G. 1988b; Genetic control of scrapie in Cheviot and Suffolk sheep. Veterinary Record 123:159
    [Google Scholar]
  10. Foster J. D., Dickinson A. G. 1989; Age at death from natural scrapie in a flock of Suffolk sheep. Veterinary Record 125:415–417
    [Google Scholar]
  11. Foster J. D., Hunter N. 1991; Partial dominance of the Sip gene in the control of experimental scrapie in Cheviot sheep. Veterinary Record (in press)
    [Google Scholar]
  12. Goldmann W., Hunter N., Foster J. D., Salbaum J. M., Beyreuther K., Hope J. 1990; Two alleles of a neural protein gene linked to scrapie in sheep. Proceedings of the National Academy of Sciences, U.S.A 87:2476–2480
    [Google Scholar]
  13. Gordon W. S. 1966; Variation in susceptibility of sheep to scrapie and genetic implications. In Report of Scrapie Seminar 1964ARS91–53 U. S. Department of Agriculture;244
    [Google Scholar]
  14. Hsiao K., Baker H. F., Crow T. J., Poulter M., Owen F., Terwilliger J. D., Westaway D., Ott J., Prusiner S. B. 1989; Linkage of a prion protein missense variant to Gerstmann-Straussler syndrome. Nature, London 338:342–345
    [Google Scholar]
  15. Hunter N., Hope J., McConnell I., Dickinson A. G. 1987; Linkage of the scrapie-associated fibril protein (PrP) gene and Sinc using congenic mice and restriction fragment polymorphism analysis. Journal of General Virology 68:2711–2716
    [Google Scholar]
  16. Hunter N., Foster J. D., Dickinson A. G., Hope J. 1989; Linkage of the gene for the scrapie-associated fibril protein (PrP) to the Sip gene in Cheviot sheep. Veterinary Record 124:364–366
    [Google Scholar]
  17. Parry H. B. 1984 Scrapie London: Academic Press;
    [Google Scholar]
  18. Scott M., Foster D., Mirenda C., Serban D., Confal F., Walchli M., Torchis M., Groth D., Carlson G., DeArmond S. J., Westaway D., Prusiner S. B. 1989; Transgenic mice expressing hamster prion protein produce species-specific scrapie infectivity and amyloid plaques. Cell 59:847–857
    [Google Scholar]
  19. Westaway D., Goodman P. A., Mirenda C. A., McKinley M. P., Carlson G. A., Prusiner S. B. 1987; Distinct prion proteins in short and long scrapie incubation period mice. Cell 51:651–662
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-72-6-1287
Loading
/content/journal/jgv/10.1099/0022-1317-72-6-1287
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error