1887

Abstract

Chemical and enzymic cleavages of the F subunit of the fusion (F) protein of respiratory syncytial (RS) virus showed that the sequence 184-Gly to 314-Trp reacted with neutralizing monoclonal antibodies (MAbs). Twelve synthetic peptides covering a part of this sequence were analysed for their immunoreactivity with neutralizing MAbs and anti-RS virus rabbit serum. Two sequential antigenic domains corresponding to amino acids 200 to 225 and 255 to 278 were defined with anti-RS virus rabbit serum. The peptides 205–225 and 259–278, belonging to these antigenic domains, inhibited binding to the F protein and the neutralizing activity of the anti-RS virus rabbit serum. One MAb (RS-348) reacted with peptides containing amino acids 200 to 225. Moreover, the peptide 205–225 induced an anti-peptide rabbit serum neutralizing RS virus . These results indicate that the sequence from residues 200 to 225 was present in one of the immunodominant sites of the F protein.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-5-1051
1991-05-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/5/JV0720051051.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-5-1051&mimeType=html&fmt=ahah

References

  1. Anderson L. J., Hierholzer J. C., Tsou C., Hendry R. M., Fernie B. F., Stone Y., McIntosh K. 1985; Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. Journal of Infectious Diseases 151:626–633
    [Google Scholar]
  2. Atherton E., Sheppard R. C. 1985; Solid phase peptide synthesis using Nα -fluorenylmethoxycarbonylamino acid pentafluorophenyl esters. Journal of the Chemical Society, Chemical Communications 3:165–166
    [Google Scholar]
  3. Brideau R. J., Walters R. R., Stier M. A., Wathen M. W. 1989; Protection of cotton rats against respiratory syncytial virus by vaccination with a novel chimeric FG glycoprotein. Journal of General Virology 70:2637–2644
    [Google Scholar]
  4. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  5. Collins P. L., Huang Y. T., Wertz G. L. 1984; Nucleotide sequence of the gene encoding the fusion (F) glycoprotein of human respiratory syncytial virus. Proceedings of the National Academy of Sciences, U.S.A 81:7683–7687
    [Google Scholar]
  6. Elango N., Satake M., Coligan J. E., Norrby E., Camargo E., Venkatesan S. 1985; Respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mRNA, identification of cleavage activation site and amino acid sequence of N-terminusof FI subunit. Nucleic Acids Research 13:1559–1574
    [Google Scholar]
  7. Garnier J., Osguthorpe D. J., Robson B. 1978; Analysis of the accuracy and implications of simple method for predicting the secondary structure of globular proteins. Journal of Molecular Biology 120:97–120
    [Google Scholar]
  8. Hopp T. P., Woods K. R. 1981; Prediction of protein antigenic determinants from amino acid sequences. Proceedings of the National Academy of Sciences, U.S.A 78:3824–3828
    [Google Scholar]
  9. Janin L. 1979; Surface and inside volumes in globular proteins. Nature, London 277:491–492
    [Google Scholar]
  10. Johnson P. R., Collins P. L. 1988; The fusion glycoproteins of human respiratory syncytial virus of subgroups A and B: sequence conservation provides a structural basis for antigenic relatedness. Journal of General Virology 69:2623–2628
    [Google Scholar]
  11. Johnson P. R., Olmsted R. A., Prince G. A., Murphy B. R., Alling D. W., Walsh E. E., Collins P. L. 1987a; Antigenic relatedness between the glycoproteins of human respiratory syncytial virus subgroups A and B: evaluation of the contributions of the F and G glycoproteins to immunity. Journal of Virology 61:3163–3166
    [Google Scholar]
  12. Johnson P. R., Spriggs M. K., Olmsted R. A., Collins P. L. 1987b; The G glycoprotein of human respiratory syncytial viruses of subgroups A and B: extensive sequence divergence between antigenically related proteins. Proceedings of the National Academy of Sciences, U.S.A 84:5625–5629
    [Google Scholar]
  13. Karplus P. A., Schulz G. E. 1985; Prediction of chain flexibility in proteins: a tool for the selection of peptide antigens. Naturwissen- schaften 72:212–213
    [Google Scholar]
  14. Kim H. W., Arrobio J. O., Brandt C. D., Jeffries B. C., Pyles G., Reid J. L., Chanock R. M., Parrott R. H. 1973; Epidemiology of RSV infection in Washington, D.C. I. Importance of virus in different respiratory tract disease syndromes and temporal distribution of infection. American Journal of Epidemiology 98:216–225
    [Google Scholar]
  15. Kusters J. G., Jager E. J., Lenstra J. A., Koch G., Posthumus W. P. A., Meloen R. H., van der Zeijst B. A. M. 1989; Analysis of an immunodominant region of infectious bronchitis virus. Virology 143:2692–2698
    [Google Scholar]
  16. Levely M. E., Mitchell M. A., Nicholas J. A. 1990; Synthetic immunogens constructed from T-cell and B-Cell stimulating peptides (T:B chimeras): preferential stimulation of unique T-and B- cell specificities is influenced by immunogen configuration. Cellular Immunology 125:65–78
    [Google Scholar]
  17. Lischwe M. A., Ochs D. 1982; A new method for partial peptide mapping using Af-chlorosuccinimide/urea and peptide silver staining in sodium dodecyl sulfate–polyacrylamide gels. Analytical Biochemistry 127:453–457
    [Google Scholar]
  18. López J. A., Peñas C., García-Barreno B., Melero J. A., Portela A. 1990; Location of a highly conserved neutralizing epitope in the F glycoprotein of human respiratory syncytial virus. Journal of Virology 64:927–930
    [Google Scholar]
  19. Mufson M. A., Örvell C., Rafnar B., Norrby E. 1985; Two distinct subtypes of human respiratory syncytial virus. Journal of General Virology 66:2111–2124
    [Google Scholar]
  20. Olmsted R. A., Elango N., Prince G. A., Murphy B. R., Johnson P. R., Moss B., Chanock R. M., Collins P. L. 1986; Expression of the F glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the F and G glycoproteins to host immunity. Proceedings of the National Academy of Sciences, U.S.A 83:7462–7466
    [Google Scholar]
  21. Rittenhouse J., Marcus F. 1984; Peptide mapping by polyacrylamide gel electrophoresis after cleavage at aspartyl-prolyl peptide bonds in sodium dodecyl sulfate-containing buffers. Analytical Biochemistry 138:442–448
    [Google Scholar]
  22. Saris C. J. M., Van Eenbergen J., Jenks B. G., Bloemers M. P. J. 1983; Hydroxylamine cleavage of proteins in polyacrylamide gels. Analytical Biochemistry 127:453–157
    [Google Scholar]
  23. Satake M., Coligan J. E., Elango N., Norrby E., Venkatesan S. 1985; Respiratory syncytial virus envelope glycoprotein has a novel structure. Nucleic Acids Research 13:7795–7812
    [Google Scholar]
  24. Scopes G. E., Watt P. J., Lambden P. R. 1990; Identification of a linear epitope on the fusion glycoprotein of respiratory syncytial virus. Journal of General Virology 71:53–59
    [Google Scholar]
  25. Stott E. J., Taylor G., Ball L. A., Anderson K., Young K. K. Y., King A. M. Q., Wertz G. W. 1987; Immune and histopathological responses in animals vaccinated with recombinant vaccinia viruses that express individual genes of human respiratory syncytial virus. Journal of Virology 61:3855–3861
    [Google Scholar]
  26. Trudel M., Nadon F., Seguin C., Dionne G., Lacroix M. 1987; Identification of a synthetic peptide as a part of a major neutralization epitope of respiratory syncytial virus. Journal of General Virology 68:2273–2280
    [Google Scholar]
  27. Walsh E. E., Hruska J. 1983; Monoclonal antibodies to respiratory syncytial virus proteins: identification of the fusion protein. Journal of Virology 47:171–177
    [Google Scholar]
  28. Walsh E. E., Schlesinger J. J., Brandriss M. W. 1984a; Purification and characterization of GP90, one of the envelope glycoprotein subunits of respiratory syncytial virus. Journal of General Virology 65:761–767
    [Google Scholar]
  29. Walsh E. E., Schlesinger J. J., Brandriss M. W. 1984b; Protection from respiratory syncytial virus infection in cotton rats by passive transfer of monoclonal antibodies. Infection and Immunity 43:756–758
    [Google Scholar]
  30. Walsh E. E., Brandriss M. W., Schlesinger J. J. 1985; Purification and characterization of the respiratory syncytial virus fusion protein. Journal of General Virology 66:409–415
    [Google Scholar]
  31. Walsh E. E., Brandriss M. W., Schlesinger J. J. 1987; Immunological differences between the envelope glycoproteins of two strains of human respiratory syncytial virus. Journal of General Virology 68:2169–2176
    [Google Scholar]
  32. Wertz G. W., Collins P. L., Huang Y., Gruber C., Levine S., Ball L. A. 1985; Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. Proceedings of the National Academy of Sciences, U.S.A 82:4075–4079
    [Google Scholar]
  33. Wertz G. W., Stott E. J., Young K. K. Y., Anderson K., Ball L. A. 1987; Expression of the fusion protein of human respiratory syncytial virus from recombinant vaccinia virus vectors and protection of vaccinia mice. Journal of Virology 61:293–301
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-72-5-1051
Loading
/content/journal/jgv/10.1099/0022-1317-72-5-1051
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error