1887

Abstract

The culture of parvovirus B19 in foetal liver tissue has been described recently. We have established the technique in our laboratory and studied parameters affecting the yield of B19 virus. Replication of the virus was detected by radioimmunoassay for B19 antigen and dot blot hybridization assay of B19 DNA, and the virus was localized by immunofluorescence and thin section electron microscopy. B19 DNA and antigen production became detectable at day 2 and reached a maximum at day 5. Virus particles were seen mainly in cell nuclei, but some cytoplasmic membranes were lined with virus particles. The amount of virus produced depended on the age of the foetus and the cell culture and the concentration of erythropoietin and interleukin 3 in the culture medium.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-3-741
1991-03-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/3/JV0720030741.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-3-741&mimeType=html&fmt=ahah

References

  1. Anderson M. J., Jones S. E., Fisher-Hoch S. P., Lewis E., Hall S. M., Bartlett C. L. R., Cohen B. J., Mortimer P. P., Pereira M. S. 1983; Human parvovirus, the cause of erythema infectiosum (fifth disease)?. Lancet i:1378
    [Google Scholar]
  2. Beard C., St Amand J., Astell C. R. 1989; Transient expression of B19 parvovirus gene products in COS-7 cells transfected with B19-SV40 hybrid vectors. Virology 172:659–664
    [Google Scholar]
  3. Brown C. S., Salimans M. M. M., Noteborn M. H. M., Weiland H. T. 1990; Antigenic parvovirus B19 coat proteins VP1 and VP2 produced in large quantities in a baculovirus expression system. Virus Research 15:197–211
    [Google Scholar]
  4. Brown K. E. 1989; What threat is human parvovirus B19 to the foetus? A review. British Journal of Obstetrics and Gynaecology 96:764–767
    [Google Scholar]
  5. Cohen B. J., Mortimer P. P., Pereira M. S. 1983; Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV). Journal of Hygiene 91:113–130
    [Google Scholar]
  6. Cossart Y. E., Field A. M., Cant B., Widdows D. 1975; Parvovirus-like particles in human sera. Lancet i:72–73
    [Google Scholar]
  7. Fridell E., Trojnar J., Wahren B. 1989; A new peptide for human parvovirus B19 antibody detection. Scandinavian Journal of Infectious Diseases 21:597–603
    [Google Scholar]
  8. Kajigaya S., Shimada T., Fujita S., Young N. S. 1989; A genetically engineered cell line that produces empty capsids of B19 (human) parvovirus. Proceedings of the National Academy of Sciences, U.S.A. 86:7601–7605
    [Google Scholar]
  9. Kurtzman G. J., Ozawa K., Cohen B., Hanson G., Oseas R., Young N. S. 1987; Chronic bone marrow failure due to peristent B19 parvovirus infection. New England Journal of Medicine 317:287–294
    [Google Scholar]
  10. Lyon D. J., Chapman C. S., Martin C., Brown K. E., Clewley J. P., Flower A. J. E., Mitchell V. E. 1989; Symptomatic parvovirus B19 infection and heat-treated factor IX concentrate. Lancet i:1085
    [Google Scholar]
  11. Mori J., Field A. M., Clewley J. P., Cohen B. J. 1989; Dot blot hybridization assay of B19 virus DNA in clinical specimens. Journal of Clinical Microbiology 27:459–464
    [Google Scholar]
  12. Mortimer P. P., Humphries R. K., Moore J. G., Purcell R. H., Young N. S. 1983; A human parvovirus-like virus inhibits haematopoietic colony formation in vitro . Nature, London 302:426–429
    [Google Scholar]
  13. Ozawa K., Kurtzman G., Young N. 1987; Productive infection by B19 parvovirus of human erythroid bone marrow cells in vitro . Blood 70:384–391
    [Google Scholar]
  14. Pattison J. R., Jones S. E., Hodgson J., Davis L. R., White J. M., Stroud C. E., Murtara L. 1981; Parvovirus infections and hypoplastic crisis in sickle cell anaemia. Lancet i:664–665
    [Google Scholar]
  15. Russell E. S., Smith L. J., Lawson F. A. 1956; Implantation of normal blood-forming tissue in radiated genetically anaemic hosts. Science 124:1076–1077
    [Google Scholar]
  16. Sisk W. P., Berman M. L. 1987; Expression of human parvovirus B19 structural protein in E. coli and detection of antiviral antibodies in human serum. Bio/Technology 5:1077–1080
    [Google Scholar]
  17. Takahashi T., Ozawa K., Takahashi K., Asano S., Takaku F. 1990; Susceptibility of human erythropoietic cells to B19 parvovirus in vitro increases with differentiation. Blood 75:603–610
    [Google Scholar]
  18. Wintrobe M. M., Lee G. R., Boggs D. R., Bithell T. C., Foerster J., Athens J. W., Lukens J. N. 1981; Origin and development of the blood and blood forming tissues. In Clinical Hematology pp 35–74 Philadelphia: Lea and Febiger;
    [Google Scholar]
  19. Yaegashi N., Shiraishi H., Takeshita T., Nakamura M., Yajima A., Sugamura K. 1989; Propagation of human parvovirus B19 in primary culture of erythroid lineage cells derived from fetal liver. Journal of Virology 63:2422–2426
    [Google Scholar]
  20. Young N., Harrison M., Moore J., Mortimer P., Humphries R. K. 1984; Direct demonstration of the human parvovirus in erythroid progenitor cells infected in in vitro . Journal of Clinical Investigation 74:2024–2032
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-3-741
Loading
/content/journal/jgv/10.1099/0022-1317-72-3-741
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error