1887

Abstract

Young leaves of tobacco, systemically infected by tobacco etch potyvirus (TEV), were examined for the presence and distribution of four virus encoded proteins [capsid, cytoplasmic inclusion (CI) and two nuclear inclusion (NI) proteins] at various time periods after inoculation of expanded leaves of the plants. The analyses were carried out by ELISA and by immunogold electron microscopy of thin sections of the leaves. All four proteins were detected simultaneously in the systemic leaves for the first time on the fifth day after inoculation of the expanded leaves. All four proteins increased in concentration until the seventh day and then showed no further increase with the exception of the capsid protein which continued to accumulate. The CI protein was first detected in association with the plasmalemma/cell wall and was subsequently found mostly in the form of pinwheels in the cytoplasm. The two NI proteins were found at all times after infection within the nucleus, although small concentrations were detected in the cytoplasm. These experiments suggest that both the NIa and NIb proteins are transported into the nucleus immediately after synthesis. At the earliest time periods after infection, high concentrations of these proteins (NIa and NIb) were found in their noninclusion form in the nucleolus. At 14 days after infection, both proteins were found only as inclusions in the nucleus. The capsid protein was found at all stages of infection only in the cytoplasm.

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1991-03-01
2022-01-25
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References

  1. Allison R., Johnston R. E., Dougherty W. G. 1986; The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154:9–20
    [Google Scholar]
  2. Andrews J. H., Shalla T. A. 1974; The origin, development and conformation of amorphous inclusion body components in tobacco etch virus-infected cells. Phytopathology 64:1234–1243
    [Google Scholar]
  3. Baunoch D. A., Das P., Hari V. 1988; Intracellular localization of TEV capsid and inclusion proteins by immunogold labeling. Journal of Ultrastructure and Molecular Structure Research 99:203–212
    [Google Scholar]
  4. Baunoch D. A., Das P., Hari V. 1990; Potato virus Y helper component protein is associated with amorphous inclusions. Journal of General Virology 71:2479–2482
    [Google Scholar]
  5. Carrington J. C., Dougherty W. G. 1987; Small nuclear inclusion protein encoded by a plant potyviral genome is a protease. Journal of Virology 61:2540–2548
    [Google Scholar]
  6. DeMejia M. V. G., Hiebert E., Purcifull D. E. 1985a; Isolation and partial characterization of the amorphous cytoplasmic inclusions associated with infections caused by two potyviruses. Virology 142:24–33
    [Google Scholar]
  7. DeMejia M. V. G., Hiebert E., Purcifull D. E., Thornbury D., Pirone T. P. 1985b; Identification of potyviral amorphous inclusion protein as a non-structural virus-specific protein related to helper component. Virology 142:34–43
    [Google Scholar]
  8. Domier L. L., Shaw J. G., Rhoads R. E. 1987; Potyviral proteins share amino acid sequence homology with picorna-, como-, and caulimoviral proteins. Virology 158:20–27
    [Google Scholar]
  9. Dougherty W. G., Carrington J. C. 1988; Expression and function of potyviral gene proteins. Annual Review of Phytopathology 26:123–218
    [Google Scholar]
  10. Edwardson J. R. 1974; Some properties of the potato virus Y group. Florida Agricultural Experimental Station Monographs Series: No. 4 398 pp
    [Google Scholar]
  11. Engvall E. 1980; Enzyme immunoassay, ELISA and EMIT. Methods in Enzymology 70:419–439
    [Google Scholar]
  12. Gadh I. P. S., Hari V. 1986; Association of tobacco etch virus related RNA with chloroplasts in extracts of infected plants. Virology 160:304–307
    [Google Scholar]
  13. Gardner W. S. 1969; Ultrastructure of Zea mays leaf cells infected with Johnson-grass strain of sugarcane mosaic virus. Phytopathology 59:1903–1907
    [Google Scholar]
  14. Gorbalenya A. E., Blinow V. M., Dochenko A. P., Koonin E. V. 1989; An NTP-binding motif is the most conserved sequence in a highly diverged monophyletic group of proteins involved in positive strand RNA viral replication. Journal of Molecular Evolution 28:256–268
    [Google Scholar]
  15. Hiebert E., McDonald J. G. 1973; Characterization of some proteins associated with viruses in the potato virus Y group. Virology 56:349–361
    [Google Scholar]
  16. Hiebert E., Purcifull D. E., Christie R. G. 1984; Purification and immunological analyses of plant viral inclusion bodies. In Methods in Virology vol 8 pp 225–278 Edited by Maramorosch K., Koprowski H. New York: Academic Press;
    [Google Scholar]
  17. Kassanis B. 1939; Intranuclear inclusions in virus infected plants. Annals of Applied Biology 26:705–709
    [Google Scholar]
  18. Knuhtsen H., Hiebert E., Purcifull D. E. 1974; Partial purification and some properties of tobacco etch virus-induced intranuclear inclusions. Virology 61:200–209
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  20. Laín S., Riechmann J. L., Garcia J. A. 1989a; Homologous potyvirus and flavivirus proteins belonging to a superfamily of helicase-like proteins. Gene 82:357–362
    [Google Scholar]
  21. Laín S., Riechmann J. L., Garcia J. A. 1989b; The complete nucleotide sequence of plum pox potyvirus RNA. Virus Research 13:157–172
    [Google Scholar]
  22. Langenberg W. G. 1986; Virus protein association with cylindrical inclusions of two viruses that infect wheat. Journal of General Virology 61:1161–1168
    [Google Scholar]
  23. Lawson R. H., Hearon S. S. 1971; The association of pinwheel inclusions with plasmodesmata. Virology 44:454–456
    [Google Scholar]
  24. Murphy J. F., Rhoads R. E., Hunt A. G., Shaw J. G. 1990; The VPg of tobacco etch virus RNA is the 49kDa proteinase or the N-terminal 24kDa part of the proteinase. Virology 178:285–288
    [Google Scholar]
  25. Purcifull D. E., Hiebert E. 1982; Tobacco etch virus. CMI/AAB Description of Plant Viruses no:258
    [Google Scholar]
  26. Rueckert R. R. 1989; Picornaviridae and their replication. In Virology vol 1 pp 507–545 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  27. Sheffield F. M. L. 1941; The cytoplasmic and nuclear inclusions associated with severe etch virus. Journal of the Royal Microscopical Society 61:30–45
    [Google Scholar]
  28. Soprano K. J., Galanti N., Jonak G. T., McKercher S., Pipas J. M., Peden K. W. C., Baserga R. 1983; Mutational analysis of simian virus 40 T-antigen: stimulation of cellular DNA synthesis and activation of r-RNA genes by mutation with deletions in the T-antigen gene. Molecular and Cellular Biology 3:214–219
    [Google Scholar]
  29. Weintraub M., Ragetli H. W. T., Leung E. 1976; Elongated virus particles in plasmodesmata. Journal of Ultrastructure Research 56:351–364
    [Google Scholar]
  30. Winston S. E., Fuller S. A., Hurrell J. G. R. 1988; Enzyme-linked immunosorbent assays (ELISA) for detection of antigens. In Current Protocols in Molecular Biology unit 11.2 Edited by Ausubel F. M. New York: Wiley Interscience;
    [Google Scholar]
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