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A nucleotide sequence is reported for RNA-3, the smallest of the three major RNA species found in particles of raspberry bushy dwarf virus (RBDV). The sequence of 946 nucleotides contains a single large open reading frame which encodes an M r 30509 polypeptide. In vitro translation of RNA-3 yielded an M r 30000 product that reacted specifically with antiserum to RBDV particles and we conclude that the amino acid sequence deduced from the sequence of RNA-3 is that of the RBDV coat protein, or an immediate precursor of it. No affinities were detected by comparing the nucleotide sequence of RNA-3 with the sequences of other plant viruses.
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