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Volume 72, Issue 2

Integrated bovine leukosis proviral DNA in T helper and T cytotoxic/suppressor lymphocytes Free

Abstract

Bovine leukosis virus (BLV) is associated with the disease complex enzootic bovine leukosis. The infection may remain clinically silent in the form of an aleukaemic state or emerge as a persistent lymphocytosis and more rarely as lymphosacroma. BLV has been considered classically to be a B lymphotropic virus, based upon the absolute increase in B lymphocytes in persistent lymphocytosis, the B lymphocyte phenotype of a majority of the cells making up lymphosarcomas and the identification of viral antigen expressed in B lymphocytes following culture of peripheral blood mononuclear leukocytes. This association of BLV with B lymphocytes is well established but the mechanism(s) of disease expression is not defined. To examine further the cellular tropism(s) of BLV, T lymphocyte subpopulations from 10 lymphocytotic cattle were established . Lymphocyte cultures were characterized by their subpopulation phenotype and DNA was extracted for identification of integrated provirus by Southern blot hybridization. Provirus was identified in T lymphocyte cultures derived from seven of 10 lymphocytotic cattle, with both T helper and T cytotoxic/suppressor subpopulations affected.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1991
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1991-02-01
2024-04-26
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Integrated bovine leukosis proviral DNA in T helper and T cytotoxic/suppressor lymphocytes
J. Gen. Virol. 72, 307 (1991); https://doi.org/10.1099/0022-1317-72-2-307
/content/journal/jgv/10.1099/0022-1317-72-2-307
/content/journal/jgv/10.1099/0022-1317-72-2-307
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