Larvae of the pine emperor moth, , infected with Nudaurelia β virus (NβV) consistently contained one species of dsRNA. This dsRNA was the correct size to be the replicative form of the NβV genome and, in Northern blots, it hybridized with P-end-labelled virion RNA. Other smaller dsRNAs were obtained in a non-reproducible manner but these had no sequences in common with the genomic probe; no dsRNAs were observed in extracts from virus-free larvae. Cell-free translation of NβV RNA resulted in the synthesis of only one major polypeptide, of about 71000, which could not be precipitated by an anti-NβV serum.


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