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Larvae of the pine emperor moth, Nudaurelia cytherea capensis, infected with Nudaurelia β virus (NβV) consistently contained one species of dsRNA. This dsRNA was the correct size to be the replicative form of the NβV genome and, in Northern blots, it hybridized with 32P-end-labelled virion RNA. Other smaller dsRNAs were obtained in a non-reproducible manner but these had no sequences in common with the genomic probe; no dsRNAs were observed in extracts from virus-free larvae. Cell-free translation of NβV RNA resulted in the synthesis of only one major polypeptide, of M r about 71000, which could not be precipitated by an anti-NβV serum.
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