1887

Abstract

Human cytomegalovirus (HCMV) purified from urine or tissue culture supernatant has been reported to contain β-microglobulin (β2m), which forms the light chain of HLA class I molecules. It has been postulated that HCMV covered with β2m binds to HLA class I α-chains at the cell surface. In the present study we used transfected human and mouse cell lines expressing distinct allelic forms of HLA class I and β2m to determine whether HLA class I molecules could act as cellular receptors for HCMV. The susceptibility of cells to HCMV infection was estimated by calculating the percentage of cells expressing HCMV immediate early antigens. Although the results showed some variation between different transfected cell clones, no correlation was found between expression of HLA class I on the cell membrane and HCMV infection. Preincubation of HLA class I-positive cells with antibodies against HLA class I antigens inhibited HCMV infection after binding and adsorption of HCMV. Trypsin prevented HCMV infection of both class I-positive and class I-negative cells. We conclude that these results do not support the assumption that HLA class I molecules are functional receptors for HCMV.

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1991-11-01
2021-10-25
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References

  1. Akiyama Y., Zicht R., Ferrone S., Bonnard G. D., Herberman R. B. 1985; Effect of monoclonal antibodies (MoAb) to class I and class II HLA antigens in lectin- and MoAb OKT3-induced lymphocyte proliferation. Cellular Immunology 91:477–491
    [Google Scholar]
  2. Albrecht T., Boldogh I., Fons M., Lee C. H., AbuBakar S., Russell J. M., Au W. W. 1989; Cell-activation responses to cytomegalovirus infection: relationship to the phasing of CMV replication and to the induction of cellular damage. Subcellular Biochemistry 15:157–202
    [Google Scholar]
  3. Atwood J. W., Norkin L. C. 1989; Class I major histocompatibility proteins as cell surface receptors for simian virus 40. Journal of Virology 63:4474–4477
    [Google Scholar]
  4. Barnstable C. J., Bodmer W. F., Brown G., Galfre B. B., Milstein C., Williams A. F., Ziegler A. 1978; Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - new tools for genetic analysis. Cell 14:9–20
    [Google Scholar]
  5. Beck S., Barrell B. G. 1988; Human cytomegalovirus encodes a glycoprotein homologous to MHC class-I antigens. Nature, London 331:269–272
    [Google Scholar]
  6. Bernabeu C., van de Rijn M., Lerch P. G., Terhorst C. P. 1984; β 2-microglobulin from serum associates with MHC class I antigens on the surface of cultured cells. Nature, London 308:642–645
    [Google Scholar]
  7. Boom R., Sol C. J. A., Minnaar R. P., Geelen J. L. M. C., Raap A. K., van der Noordaa J. 1988; Induction of gene expression under human cytomegalovirus immediate early enhancer-promoter control by inhibition of protein synthesis is cell cycle-dependent. Journal of General Virology 69:1179–1193
    [Google Scholar]
  8. Brodsky F. M., Parham P., Barnstable C. J., Crumpton M. J., Bodmer W. F. 1979; Monoclonal antibodies for analysis of the HLA system. Immunological Reviews 47:3–61
    [Google Scholar]
  9. Browne H., Smith G., Beck S., Minson T. 1990; A complex between the MHC class I homologue encoded by human cytomegalo-virus and β2 microglobulin. Nature, London 347:770–772
    [Google Scholar]
  10. Corbeau P., Devaux C., Kourilsky F., Chermann J. 1990; An early postinfection signal mediated by monoclonal anti-β 2 microglobulin antibody is responsible for delayed production of human immunodeficiency virus type 1 in peripheral blood mononuclear cells. Journal of Virology 64:1459–1465
    [Google Scholar]
  11. De Leij L., Postmus P. E., Buys C. H. C. M., Elema J. D., Ramaekers F., Poppema S., Brouwer M., van der Veen A. Y., Mesander G., The T. H. 1985; Characterization of three new variant type cell lines derived from small cell carcinoma of the lung. Cancer Research 45:6024–6033
    [Google Scholar]
  12. De Preval C., Mach B. 1983; The absence of β 2-microglobulin in Daudi cells: active gene but inactive messenger RNA. Immunogenetics 17:133–140
    [Google Scholar]
  13. Ellis S. A., Taylor C., McMichael A. 1982; Recognition of HLA-B27 and related antigen by a monoclonal antibody. Human Immunology 5:49–59
    [Google Scholar]
  14. Gleaves C. A., Smith T. F., Shuster E. A., Pearson G. R. 1984; Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low speed centrifugation and monoclonal antibody to an early antigen. Journal of Clinical Microbiology 19:917–919
    [Google Scholar]
  15. Grundy J. E., McKeating J. A., Griffiths P. D. 1987a; Cytomegalovirus strain AD 169 binds # 2 microglobulin in vitro after release from cells. Journal of General Virology 68:777–784
    [Google Scholar]
  16. Grundy J. E., McKeating J. A., Ward P. J., Sanderson A. R., Griffiths P. D. 1987b; β2 Microglobulin enhances the infectivity of cytomegalovirus and when bound to the virus enables class IHLA molecules to be used as a virus receptor. Journal of General Virology 68:793–803
    [Google Scholar]
  17. Ho M. 1982 In Cytomegalovirus: Biology and Infection pp 119–169 Edited by Greenough W. R. III, Merigan T. C. New York: Plenum Press;
    [Google Scholar]
  18. Hyafil F., Strominger J. L. 1979; Dissociation and exchange of the β 2-microglobulin subunit of HLA-A and HLA-B antigens. Proceedings of the National Academy of Sciences, U.S.A. 76:5834–5838
    [Google Scholar]
  19. Keay S., Merigan T. C., Rasmussen L. 1989; Identification of cell surface receptors for the 86-kilodalton glycoprotein of human cytomegalovirus. Proceedings of the National Academy of Sciences, U.S.A. 86:10100–10103
    [Google Scholar]
  20. McKeating J. A., Griffiths P. D., Grundy J. E. 1987; Cytomegalovirus in urine specimens has host β 2 microglobulin bound to the viral envelope: a mechanism of evading the host immune response?. Journal of General Virology 68:785–792
    [Google Scholar]
  21. Neefjes J. J., Ploegh H. L. 1988; Allele and locus-specific differences in cell surface expression and the association of class I heavy chain with β 2-microglobulin: differential effects of inhibition of glycosylation on class I subunit association. European Journal of Immunology 18:801–810
    [Google Scholar]
  22. Parham P., Brodsky F. M. 1981; Partial purification and some properties of BB7.2. Human Immunology 3:277–299
    [Google Scholar]
  23. Price P., Winter J. G., Nikoletti S., Hudson J. B., Shellam G. R. 1987; Functional changes in murine macrophages infected with cytomegalovirus relate to H-2-determined sensitivity to infection. Journal of Virology 61:3602–3606
    [Google Scholar]
  24. Price P., Gibbons A. E., Shellam G. R. 1990; H-2 class I loci determine sensitivity to MCMV in macrophages and fibroblasts. Immunogenetics 32:20–26
    [Google Scholar]
  25. Rossmann M. G., Arnold E., Erickson J. W., Frankenberger E. A., Griffith J. P., Hecht H. J., Johnson J. E., Kamer G., Luo M., Mosser A. G., Rueckert R. R., Sherry B., Vriend G. 1985; Structure of a human common cold virus and functional relationship to other picomaviruses. Nature, London 317:145–153
    [Google Scholar]
  26. Sachs D. H., Mayer N., Ozato K. 1981; Hybridoma antibodies directed toward murine H-2 and Ia antigens. In Monoclonal Antibodies and T Cell Hybridomas: Perspectives and Technical Advances p 95 Edited by Hammerling G. J., Hammerling U., Kearney J. F. Elsevier/North-Holland; Amsterdam:
    [Google Scholar]
  27. Sakaguchi K., Ono R., Tsujisaki M., Richiardi P., Carbonara A., Park M. S., Tonai R., Terasaki P. I., Ferrone S. 1988; Anti-HLA-B7, B27, Bw42, Bw54, Bw55, Bw56, Bw67, Bw73 monoclonal antibodies: specificity, idiotypes, and application for a double determinant immunoassay. Human Immunology 21:193–207
    [Google Scholar]
  28. Stam N. J., Van der Hoeven F. A., Ploegh H. L. 1990; The class I negative phenotype of a neuroendocrine tumor is transcriptionally controlled. Journal of Immunology 142:4113–4117
    [Google Scholar]
  29. Stannard L. M. 1989; β2 Microglobulin binds to the tegument of cytomegalovirus: an immunogold study. Journal of General Virology 70:2179–2184
    [Google Scholar]
  30. Stinski M. F., Thomsen D. R., Stenberg R. M., Goldstein L. C. 1983; Organization and expression of the immediate early genes of human cytomegalovirus. Journal of Virology 46:1–14
    [Google Scholar]
  31. Taylor H. P., Cooper N. R. 1989; Human cytomegalovirus binding to fibroblasts is receptor mediated. Journal of Virology 63:3991–3998
    [Google Scholar]
  32. Taylor H. P., Cooper N. R. 1990; The human cytomegalovirus receptor on fibroblasts is a 30-kilodalton membrane protein. Journal of Virology 64:2484–2490
    [Google Scholar]
  33. Tocci M. J., St George S. C. 1979; Susceptibility of lymphoblastoid cells to infection with human cytomegalovirus. Infection and Immunity 23:418–423
    [Google Scholar]
  34. White J. M., Littman D. R. 1989; Viral receptors of the immunoglobulin superfamily. Cell 56:725–728
    [Google Scholar]
  35. Williams A. F., Barclay A. N. 1988; The immunoglobulin superfamily - domains for cell surface recognition. Annual Review of Immunology 6:381–405
    [Google Scholar]
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