1887

Abstract

To contribute to the study of the genetic variability of hepatitis C virus (HCV) we have determined the nucleotide sequence of the E2/NS1 and NS3/NS4 regions of a French isolate using the polymerase chain reaction. Comparison of these nucleotide sequences with those available for American and Japanese isolates showed a significant genetic variability: 5 to 33% and 2 to 30% at the nucleic acid and amino acid levels, respectively. The genetic variability is higher in the E2/NS1 (13 to 33% and 12 to 30% at the nucleic acid and amino acid levels, respectively) than in the NS3/NS4 (5 to 21% and 2 to 7%) regions. The sequence of the French isolate is more closely related to that of the American HCV prototype than to the Japanese HCV isolates. This study confirms the extent of HCV genetic variability.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-10-2557
1991-10-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/10/JV0720102557.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-10-2557&mimeType=html&fmt=ahah

References

  1. Alter H. J., Purcell R. H., Shih J. W., Melpolder J. C., Houghton M., Choo Q.-L., Kuo G. 1989; Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis. New England Journal of Medicine 321:1494–1500
    [Google Scholar]
  2. Choo Q.-L., Kuo G., Weiner A. J., Overby L. R., Bradley D. W., Houghton M. 1989; Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244:359–362
    [Google Scholar]
  3. Choo Q.-L., Richman K. H., Han J. H., Berger K., Lee C., Dong C., Gallegos C., Coit D., Medina-Selby A., Barr P. J., Weiner A. J., Bradley D. W., Kuo G., Houghton M. 1991; Genetic organization and diversity of the hepatitis C virus. Proceedings of the National Academy of Sciences, U.S.A 88:2451–2455
    [Google Scholar]
  4. Collett M. S., Anderson D. K., Retzel E. 1988; Comparisons of the pestivirus bovine viral diarrhoea virus with members of the Flaviviridae. Journal of General Virology 69:2637–2643
    [Google Scholar]
  5. Enomoto N., Takada A., Nakao T., Date T. 1990; There are two major types of hepatitis C virus in Japan. Biochemical and Biophysical Research Communications 170:1021–1025
    [Google Scholar]
  6. Falgout B., Bray M., Schlesinger J. J., Lai C.-J. 1990; Immunization of mice with recombinant vaccinia virus expressing authentic dengue virus non structural protein NS1 protects against lethal dengue virus encephalitis. Journal of Virology 64:4356–4363
    [Google Scholar]
  7. Hopf U., MӦller B., Küther D., Stemerowicz R., Lobek H., Lüdtke-Handjery A., Walter E., Blum H. E., Roggendorf M., Deinhardt F. 1990; Long-term follow-up of post transfusion and sporadic chronic hepatitis non-A, and non-B and frequency of circulating antibodies to hepatitis C virus (HCV). Journal of Hepatology 10:69–76
    [Google Scholar]
  8. Kato N., Ohkoshi S., Shimotohno K. 1989; Japanese isolates of the non-A, non-B hepatitis viral genome show sequence variations from the original isolate in the U.S.A. Proceedings of the Japan Academy 65:219–223
    [Google Scholar]
  9. Kato N., Hijikata M., Ootsuyama Y., Nakagawa M., Ohkoshi S., Sugimura T., Shimotohno K. 1990; Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proceedings of the National Academy of Sciences, U.S.A 87:9524–9528
    [Google Scholar]
  10. Kubo Y., Takeuchi K., Boonmar S., Katayama T., Choo Q.-L., Kuo G., Weiner A. J., Bradley D. W., Houghton M., Saito I., Miyamura T. 1989; A cDNA fragment of hepatitis C virus isolated from an implicated donor of post-transfusion non-A, non-B hepatitis in Japan. Nucleic Acids Research 17:10367–10372
    [Google Scholar]
  11. Kuo G., Choo Q.-L., Alter H. J., Gitnick G. L., Redeker A. G., Purcell R. H., Miyamura T., Dienstag J. L., Alter M. J., Stevens C. E., Tegtmeier G. E., Bonino F., Colombo M., Lee W. S., Kuo C., Berger K., Shuster J. R., Overby L. R., Bradley D. W., Houghton M. 1989; An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science 244:362–364
    [Google Scholar]
  12. Miller R. H., Purcell R. H. 1990; Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups. Proceedings of the National Academy of Sciences, U.S.A 87:2057–2061
    [Google Scholar]
  13. Miyamura T., Saito I., Katayama T., Kikuchi S., Tateda A., Houghton M., Choo Q.-L., Kuo G. 1990; Detection of antibody against antigen expressed by molecularly cloned hepatitis C virus cDNA: application to diagnosis and blood screening for posttransfusion hepatitis. Proceedings of the National Academy of Sciences, U.S.A 87:983–987
    [Google Scholar]
  14. Okamoto H., Okada S., Sugiyama Y., Yotsumoto S., Tanaka T., Yoshizawa H., Tsuda F., Miyakawa Y., Mayumi M. 1990; The 5′ terminal sequence of the hepatitis C virus genome. Japanese Journal of Experimental Medicine 60:167–177
    [Google Scholar]
  15. Reyes G. R., Purdy M. A., Kim J. P., Luk K.-C., Young L. M., Fry K. E., Bradley D. W. 1990; Isolation of a cDNA from the virus responsible for enterically transmitted non-A, non-B hepatitis. Science 1411335–1339
    [Google Scholar]
  16. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  17. Schlesinger J. J., Brandriss M. W., Walsh E. E. 1987; Protection of mice against dengue 2 virus encephalitis by immunization with the dengue 2 virus non-structural glycoprotein NS1. Journal of General Virology 68:853–857
    [Google Scholar]
  18. Strauss J. H., Strauss E. G., Hahn C. S., Rice C. M. 1987; The genomes of alphaviruses and flaviviruses: organization and translation. In The Molecular Biology of Positive Strand RNA Viruses pp 75–102 Edited by Rowlands D. J., Mayo M. A., Mahy B. W. J. New York & London: Academic Press;
    [Google Scholar]
  19. Takeuchi K., Boonmar S., Kubo Y., Katayama T., Harada H., Ohbayashi A., Choo Q.-L., Houghton M., Saito I., Miyamura T. 1990a; Hepatitis C viral cDNA clones isolated from a healthy carrier donor implicated in post transfusion non A, non-B hepatitis. Gene 91:287–291
    [Google Scholar]
  20. Takeuchi K., Kubo Y., Boonmar S., Watanabe Y., Katayama T., Choo Q.-L., Kuo G., Houghton M., Saito I., Miyamura T. 1990b; Nucleotide sequences of core and envelope genes of the hepatitis C virus genome derived directly from human healthy carriers. Nucleic Acids Research 18:4626
    [Google Scholar]
  21. Thiers V., Nakajima E. N., Kremsdorf D., Mack D., Schelle- kens H., Driss F., Goudeau A., Wands J., Sninsky J., Tiollais P., Brechot C. 1988; Transmission of hepatitis B from hepatitis B seronegative subjects. Lancet ii:1273–1276
    [Google Scholar]
  22. Weiner A. J., Brauer M. J., Rosenblatt J., Richman K. H., Tung J., Crawford K., Bonino F., Saracco G., Choo Q.-L., Houghton M., Han J. H. 1991; Variable and hypervariable domains are found in the regions of HCV corresponding to the flavi virus envelope and NS1 proteins and the pestivirus envelope glycoproteins. Virology 180:842–848
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-10-2557
Loading
/content/journal/jgv/10.1099/0022-1317-72-10-2557
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error