1887

Abstract

The sequence of the gene encoding the major neutralization antigen (VP7) of the type member (bovine virus strain B223) of the proposed serotype 10 of rotavirus has been determined. This was done using a rapid new strategy involving direct sequencing by primer extension of cDNA generated from the relevant virus gene (gene 8) using a combined reverse transcription/polymerase chain reaction. The sequence obtained is 1062 bp in length and contains a single long open reading frame capable of encoding a protein of 326 amino acids. Comparison of the sequence to that of the corresponding gene of the other major virus serotype found in cattle, serotype 6, showed that it was approximately 25% divergent at the nucleotide level and 18% divergent at the amino acid level.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-1-177
1991-01-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/1/JV0720010177.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-1-177&mimeType=html&fmt=ahah

References

  1. Chan W. K., Penaranda M. E., Crawford S. E., Estes M. K. 1986; Two glycoproteins are produced from the rotavirus neutralization gene. Virology 164:435–442
    [Google Scholar]
  2. Charpilienne A., Borras F., D’Auriol L., Galibert F., Cohen J. 1986; Sequence of the gene encoding the outer glycoprotein of the bovine rotavirus (RF strain) and comparison with homologous genes from four bovine, simian and human rotaviruses. Armais of the Institut Pasteur . /Virolology 137E:71–77
    [Google Scholar]
  3. Clarke I. N., McCrae M. A. 1983; Molecular biology of rotaviruses. VI. Sequence-specific terminal conservation in rotaviruses. Journal of General Virology 64:1877–1884
    [Google Scholar]
  4. Dyall-Smith M. L., Lazdins I., Tregar G. W., Holmes I. H. 1986; Location of the major antigenic sites involved in rotavirus serotype-specific neutralisation. Proceedings of the National Academy of Sciences, U,. S,. A 83:3465–3468
    [Google Scholar]
  5. Elleman T. C., Hoyne P. A., Dyall-Smith M. L., Holmes I. H., Azad A. A. 1983; Nucleotide sequence of the gene encoding the serotype-specific glycoprotein of UK bovine rotavirus. Nucleic Acids Research 11:4689–4701
    [Google Scholar]
  6. Estes M. K., Cohen J. 1989; Rotavirus gene structure and function. Microbiological Reviews 53:410–449
    [Google Scholar]
  7. Flewett T. H., Woode G. N. 1978; The rotaviruses. Brief review. Archives of Virology 57:1–23
    [Google Scholar]
  8. Glass R. I., Keith J., Nakagomi O., Askaa J., Kapikian A. Z., Chanock R. M., Flores J. 1985; Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhea virus rotavirus: comparison with homologous genes from four strains of human and animal rotavirus. Virology 141:292–298
    [Google Scholar]
  9. Green K. Y., Hoshino Y., Ikegami N. 1989; Sequence analysis of the gene encoding the serotype specific glycoprotein (VP7) of two new human rotavirus serotypes. Virology 168:429–433
    [Google Scholar]
  10. Higgins D. G., Sharp P. M. 1988; CLUSTAL: a package for performing multiple sequence alignments on a microcomputer. Gene 73:237–244
    [Google Scholar]
  11. Holmes I. H. 1983; Rotaviruses. In The Reoviridae pp. 359–423 Edited by Joklik W. K. New York: Plenum Press;
    [Google Scholar]
  12. Hoshino Y., Wyatt R. G., Greenberg H. B., Flores J., Kapikian A. Z. 1984; Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque reduction neutralisation. Journal of Infectious Diseases 149:694–702
    [Google Scholar]
  13. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Sereno M. M., Kapikian A. Z., Chanock R. M. 1981; Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology 112:385–390
    [Google Scholar]
  14. McCrae M. A., McCorquodale J. G. 1981; Molecular biology of rotaviruses. I. Characterization of basic growth parameters and pattern of macromolecular synthesis. Journal of Virology 39:490–496
    [Google Scholar]
  15. McCrae M. A., McCorquodale J. G. 1982a; Molecular biology of rotaviruses. II. Identification of the protein-coding assignments of the calf rotavirus genome RNA species. Virology 117:435–443
    [Google Scholar]
  16. McCrae M. A., McCorquodale J. G. 1982b; Molecular biology of rotaviruses. IV. Molecular cloning of the bovine rotavirus genome. Journal of Virology 44:1076–1079
    [Google Scholar]
  17. McCrae M. A., McCorquodale J. G. 1983; Molecular biology of rotaviruses. V. Terminal structure of viral RNA species. Virology 126:204–212
    [Google Scholar]
  18. Mackow E. R., Shaw R. D., Matsui S. M., Vo P. T., Benfield D. A., Greenberg H. B. 1988; Characterization of homotypic and heterotypic VP7 neutralization sites of rhesus rotavirus. Virology 165:511–517
    [Google Scholar]
  19. Offit P. A., Blavat G. 1986; Identification of the two rotavirus genes determining neutralization specificities. Journal of Virology 57:376–378
    [Google Scholar]
  20. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. B. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  21. Sanger F., Nicklen S., and Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U,. S,. A. 74:5463–5467
    [Google Scholar]
  22. Snodgrass D. R., Fitzgerald T., Campbell I., Scott F. M. M., Browning G. F., Miller F. L., Herring A. J., Greenberg H. B. 1990; Rotavirus serotypes 6 and 10 predominate in cattle. Journal of Clinical Microbiology 28:504–507
    [Google Scholar]
  23. Taniguchi K., Hoshino Y., Nishikawa K., Green K. Y., Maloy W. L., Morita Y., Urasawa S., Kapikian A. Z., Chanock R. M., GoRziglia M. 1988; Cross-reactive and serotype specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies. Journal of Virology 62:1870–1874
    [Google Scholar]
  24. Taniguchi K., Pongsuwanna Y., Choonthanon M., Urasawa S. 1990; Nucleotide sequence of the VP7 gene of a bovine rotavirus (strain 61A) with different serotype specificity from serotype 6. Nucleic Acids Research 18:4613
    [Google Scholar]
  25. Winship P. R. 1989; An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide. Nucleic Acids Research 17:1266
    [Google Scholar]
  26. Xu L., Harbour D., McCrae M. A. 1990; The application of polymerase chain reaction to the detection of rotaviruses in faeces. Journal of Virological Methods 27:29–38
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-1-177
Loading
/content/journal/jgv/10.1099/0022-1317-72-1-177
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error