1887

Abstract

Since several human herpesviruses, including varicella-zoster virus (VZV), have been demonstrated to transform mammalian cells , VZV was tested in a mouse model of virus-induced cervical neoplasia to determine whether it is oncogenic . Herpes simplex viruses types 1 and 2 and cytomegalovirus have been previously shown to induce cervical neoplasia in this mouse model. VZV was propagated in WI-38 cell cultures and inactivated by ultraviolet irradiation. Control material was prepared in an identical manner from uninfected cell cultures. Cotton tampons, saturated with inactivated virus or control material, were inserted into the vaginas of C57BL mice three times a week for 60 weeks. Cervical dysplasia was detected in 40 % and invasive carcinoma in 34 % of virus-exposed mice by histological examination. No lesions were detected in control animals. These observations indicate that VZV, or some product of virus-infected cells, is oncogenic for the mouse cervix.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-9-2175
1990-09-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/9/JV0710092175.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-9-2175&mimeType=html&fmt=ahah

References

  1. Anthony D. D., Wentz W. B., Reagan J. W., Heggie A. D. 1989; Induction of cervical neoplasia in the mouse by HSV-2 DNA. Proceedings of the National Academy of Sciences, U.S.A 86:4520–4524
    [Google Scholar]
  2. Dale G. E., Coleman R. M., Best J. M., Benetato B. B. B., Drew N. C., Chinn S., Papacosta A. O., Nahmias A. J. 1988; Class-specific herpes simplex virus antibodies in sera and cervical secretions from patients with cervical neoplasia: a multi-group comparison. Epidemiology and Infection 100:455–465
    [Google Scholar]
  3. Duff R., Rapp F. 1971; Oncogenic transformation of hamster embryo cells after exposure to herpes simplex virus type 2. Nature; London: 23348–50
    [Google Scholar]
  4. Duff R., Rapp F. 1973; Oncogenic transformation of hamster embryo cells after exposure to inactivated herpes simplex virus type 1. Journal of Virology 12:209–217
    [Google Scholar]
  5. Geder L., Lausch R., O’Neill F., Rapp F. 1976; Oncogenic transformation of human embryo lung cells by human cytomegalovirus. Science 192:1134–1137
    [Google Scholar]
  6. Gelb L. D., Dohner J. 1984; Varicella-zoster virus-induced transformation of mammalian cells in vitro . Journal of Investigative Dermatology 83:77s–81s
    [Google Scholar]
  7. Gelb L. D., Huang J. J., Wellinghoff W. J. 1980; Varicella-zoster virus transformation of hamster embryo cells. Journal of General Virology 51:171–177
    [Google Scholar]
  8. Gershon A. A., Raker R., Steinberg S., Topf-Oskin B., Drusin L. 1976; Antibody to varicella-zoster virus in parturient women and their offspring during the first year of life. Pediatrics 58:692–696
    [Google Scholar]
  9. Heggie A. D., Wentz W. B., Reagan J. W., Anthony D. D. 1986; Roles of cytomegalovirus and Chlamydia trachomatis in the induction of cervical neoplasia in the mouse. Cancer Research 46:5211–5214
    [Google Scholar]
  10. Henle W., Diehl V., Kohn G., zur Hausen H., Henle G. 1967; Herpes-type virus and chromosome marker in normal leukocytes after growth with irradiated Burkitt cells. Science 157:1064–1065
    [Google Scholar]
  11. Macnab J. C. M. 1987; Herpes simplex virus and human cytomegalovirus: their role in morphological transformation and genital cancers. Journal of General Virology 68:2525–2550
    [Google Scholar]
  12. Rapp F., Albrecht T. 1973; Malignant transformation of hamster embryo fibroblasts following exposure to ultraviolet-irradiated human cytomegalovirus. Virology 55:53–61
    [Google Scholar]
  13. Rapp F., Jenkins F. J. 1981; Genital cancer and viruses. Gynecologic Oncology 12:S25–S41
    [Google Scholar]
  14. Schmidt N. J., Lennette E. H. 1976; Improved yields of cell-free varicella-zoster virus. Infection and Immunity 14:709–715
    [Google Scholar]
  15. Vonka V., Kanka J., Hirsch I., Zavadova H., Krcmar M., Suchankova A., Rezacova D., Broucek J., Press M., Domorazkova E., Svoboda B., Havrankova A., Jelinek J. 1984; Prospective study on the relationship between cervical neoplasia and herpes simplex type-2 virus. II. Herpes simplex type-2 antibody presence in sera taken at enrollment. International Journal of Cancer 33:61–66
    [Google Scholar]
  16. Wentz W. B., Reagan J. W., Heggie A. D. 1975; Cervical carcinogenesis with herpes simplex virus type 2. Obstetrics and Gynecology 46:117–121
    [Google Scholar]
  17. Wentz W. B., Reagan J. W., Heggie A. D., Fu Y. S., Anthony D. D. 1981; Induction of uterine cancer with inactivated herpes simplex virus, types 1 and 2. Cancer 48:1783–1790
    [Google Scholar]
  18. Wentz W. B., Heggie A. D., Anthony D. D., Reagan J. W. 1983; Effect of prior immunization on induction of cervical cancer in mice by herpes simplex virus type 2. Science 222:1128–1129
    [Google Scholar]
  19. zur Hausen H. 1980; The role of Epstein-Barr virus in Burkitt’s lymphoma and nasopharyngeal carcinoma. In Oncogenic Viruses 2 pp. 13–24 Rapp F. Edited by Boca Raton: CRC Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-9-2175
Loading
/content/journal/jgv/10.1099/0022-1317-71-9-2175
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error