1887

Abstract

The sequence of 1390 nucleotides of the 3′-terminal region of virus S RNA was determined from cloned cDNA. This portion of the viral RNA contains two major open reading frames (ORFs) encoding proteins of 32·9K and 12·6K. The 32·9K protein, which is similar in size to the capsid protein of the virus, shares marked homology with the coat proteins of potexviruses and two other carlaviruses. The ORF, corresponding to 12·6K, nearest to the 3′-terminal poly(A) tail shows extensive similarity with the corresponding proteins of potato virus S and potato virus M. We propose that this protein may be involved in nucleic acid binding within infected cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-8-1877
1990-08-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/8/JV0710081877.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-8-1877&mimeType=html&fmt=ahah

References

  1. AbouHaidar M. G. 1988; Nucleotide sequence of the capsid protein gene and the 3′ non-coding region of papaya mosaic virus RNA. Journal of General Virology 69:219–226
    [Google Scholar]
  2. AbouHaidar M. G., Lai R. 1989; Nucleotide sequence of the 3′-terminal region of clover yellow mosaic virus RNA. Journal of General Virology 70:1871–1875
    [Google Scholar]
  3. Adams A. N., Barbara D. J. 1982; The use of F (ab′)2 -based ELISA to detect serological relationships among carlaviruses. Annals of Applied Biology 101:495–500
    [Google Scholar]
  4. Bagnall R. H., Wetter C., Larson R. H. 1959; Differential host and serological relationships of potato virus M, potato virus S, and carnation latent virus. Phytopathology 49:435–441
    [Google Scholar]
  5. Berg J. 1986; Potential metal binding domains in nucleic acid binding proteins. Science 232:485–486
    [Google Scholar]
  6. Foster G. D., Mills P. R. 1990; Detection of strains of potato virus S by nucleic acid spot hybridisation. Potato Research in press
    [Google Scholar]
  7. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  8. Harbison S. A., Forster R. L. S., Guilford P. J., Gardner R. C. 1988; Organization and interviral homologies of the coat protein gene of white clover mosaic virus. Virology 162:459–465
    [Google Scholar]
  9. Huisman M. J., Linthorst H. J. M., Bol J. F., Cornelissen B. J. C. 1988; The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses. Journal of General Virology 69:1789–1798
    [Google Scholar]
  10. Klug A., Rhodes D. 1987; ′Zinc fingers′: a novel protein motif for nucleic acid recognition. Trends in Biochemical Sciences 12:464–469
    [Google Scholar]
  11. Kuschki G. H., Koenig R., Duvel D., Kuhne H. 1978; Helenium virus S and Y - two new viruses from commercially grown Helenium hybrids. Phytopathology 68:1407–1411
    [Google Scholar]
  12. MacFarlane S. A., Taylor S. C., King D. I., Hughes G., Davies J. W. 1989; Pea early browning virus encodes four polypeptides including a putative zinc-finger protein. Nucleic Acids Research 17:2245–2260
    [Google Scholar]
  13. MacKenzie D. J., Tremaine J. H. 1988; Ultrastructural location of non-structural protein 3A of cucumber mosaic virus in infected tissue using monoclonal antibodies to a cloned chimeric fusion protein. Journal of General Virology 69:2387–2395
    [Google Scholar]
  14. MacKenzie D. J., Tremaine J. H., Stace-Smith R. 1989; Organization and interviral homologies of the 3ʹ-terminal portion of potato virus S RNA. Journal of General Virology 70:1053–1063
    [Google Scholar]
  15. Messing J., Vieira J. 1982; A new pair of Ml 3 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19:269–276
    [Google Scholar]
  16. Miller J., McLachlan A., Klug A. 1985; Repetitive zincbinding domains in the protein transcription factor III A from Xenopusoocytes. EMBO Journal 4:1609–1614
    [Google Scholar]
  17. Nevins J. R. 1983; The pathway of eucaryotic mRNA formation. Annual Review of Biochemistry 52:441–466
    [Google Scholar]
  18. Rupasov V. V., Morozov S. Yu., Kanyuka K. V., Zavriev S. K. 1989; Partial nucleotide sequence of potato virus M RNA shows similarities to potexviruses in gene arrangement and the encoded amino acid sequences. Journal of General Virology 70:1861–1869
    [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A.: 745463–5467
    [Google Scholar]
  20. Sehnke P. C., Mason A. M., Hood S. J., Lister R. M., Johnston J. E. 1989; A ′zinc-finger′-type binding domain in tobacco streak virus coat protein. Virology 168:48–56
    [Google Scholar]
  21. Short M. N., Turner D. S., March J. F., Pappin D. J. C., Parente A., Davies J. W. 1986; The primary structure of papaya mosaic virus coat protein. Virology 152:280–283
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-71-8-1877
Loading
/content/journal/jgv/10.1099/0022-1317-71-8-1877
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error