1887

Abstract

Tumour necrosis factor (TNF-) elicited an antiviral response in some cell lines (MG-63 and HEp-2) but not in others (MDBK). Cell lines that generated an antiviral response to TNF- also showed induction of a 15K protein which shared sequence homology with ubiquitin and reacted with an antibody to ubiquitin. This ubiquitin cross-reactive protein (UCRP) had been demonstrated previously to be induced by interferon. The TNF- induction of UCRP occurred at the level of transcription. TNF- induction of both the antiviral state and the 15K protein was blocked by either monoclonal or polyclonal anti--interferon (IFN-) antibody. However no measurable increase in the mRNA specific for IFN- was detected after TNF- treatment. Nonetheless, in supernatants from cell cultures, the presence of an antiviral activity inhibitable by anti-IFN- antibody indicates that these cells are making IFN- already. We conclude that the TNF- induction of antiviral activity and UCRP in cells is dependent upon the presence of constitutive low levels of IFN- in the responding cells. Furthermore TNF functions to enhance the existing IFN- activity.

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1990-08-01
2021-10-26
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