1887

Abstract

The nucleotide sequences of the large open reading frame (ORF) from segment A of three European strains of infectious bursal disease virus (IBDV) have been determined using cDNA clones. This ORF of 3036 nucleotides encodes the virion proteins as a polyprotein in the following order: VP2, VP4, VP3. The nucleotide sequences determined have been compared to each other and to the published sequence of an Australian strain. The four strains are closely related, the greatest difference between two strains being 7.7% at the nucleotide level and 2.7% at the amino acid level. Comparisons show that there is a tight cluster of amino acid changes in the virion protein VP2. This variable region corresponded to the region where binding of a neutralizing monoclonal antibody has previously been mapped. A region in the centre of the segment, corresponding to the N terminal of VP4, was found to be completely conserved. Amino acid changes were spread fairly evenly through VP3 and there was no indication of a variable region as found in VP2.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-6-1303
1990-06-01
2024-12-01
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/6/JV0710061303.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-6-1303&mimeType=html&fmt=ahah

References

  1. Azad A. A., Barrett S. A., Fahey K. J. 1985; The characterization and molecular cloning of the double-stranded RNA genome of an Australian strain of infectious bursal disease virus. Virology 143:35–44
    [Google Scholar]
  2. Azad A. A., Jagadish M. N., Brown M. A., Hudson P. J. 1987; Deletion mapping and expression in Escherichia coli of the large genomic segment of a birnavirus. Virology 161:145–152
    [Google Scholar]
  3. Azad A. A., Jagadish M. N., Fahey K. J. 1988; IBDV VP2 epitope recognised by virus neutralising and protective monoclonal antibodies. International Patent Application PCT/AU88/00206
    [Google Scholar]
  4. Baxendale W. 1976; The development of an apathogenic infectious bursal agent vaccine: field trial results. Proceedings of the 25th Western Poultry Disease Conference and 10th Poultry Health Symposium University of California, Davis, U.S.A:
    [Google Scholar]
  5. Becht H., Müller H., Müller H. K. 1988; Comparative studies on structural and antigenic properties of two serotypes of infectious bursal disease virus. Journal of General Virology 69:631–640
    [Google Scholar]
  6. Brown T. D. K., Boursnell M. E. G., Binns M. M. 1984; A leader sequence is present on mRNA A of avian infectious bronchitis virus. Journal of General Virology 65:1437–1442
    [Google Scholar]
  7. Bygrave A. C., Faragher J. T. 1970; Mortality associated with Gumboro disease. Veterinary Record 86:758–759
    [Google Scholar]
  8. Dobos P. 1979; Peptide map comparison of the proteins of IBDV. Journal of Virology 32:1046–1050
    [Google Scholar]
  9. Dobos P., Hill P. J., Hallett R., Kells D. T. C., Becht H., Teninges D. 1979; Biophysical and biochemical characterisation of five animal viruses with bisegmented double-stranded genomes. Journal of Virology 32:593–605
    [Google Scholar]
  10. Duncan R., Dobos P. 1986; The nucleotide sequence of infectious pancreatic necrosis virus (IPNV) dsRNA segment A reveals one large open reading frame encoding a precursor polyprotein. Nucleic Acids Research 14:5934
    [Google Scholar]
  11. Ernst H., Shatkin A. J. 1985; Reovirushemagglutinin mRNA codes for two polypeptides in overlapping reading frames. Proceedings of the National Academy of Sciences, U.S.A 82:48–52
    [Google Scholar]
  12. Fahey K. J., O’Donnell I. J., Azad A. A. 1985a; Characterization by Western blotting of the immunogens of infectious bursal disease virus. Journal of General Virology 66:1479–1488
    [Google Scholar]
  13. Fahey K. J., O’Donnell I. J., Bagust T. J. 1985b; Antibody to the 32K structural protein of infectious bursal disease virus neutralizes viral infectivity in vitro and confers protection on young chickens. Journal of General Virology 66:2693–2702
    [Google Scholar]
  14. Fahey K. J., Erny K., Crooks J. 1989; A conformational immunogen on VP-2 of infectious bursal disease virus that induces virus-neutralizing antibodies that passively protect chickens. Journal of General Virology 70:1473–1481
    [Google Scholar]
  15. Feinberg A. D., Vogelstein B. 1983; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  16. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  17. Guo L., Yang R. C. A., Wu R. 1983; An improved strategy for rapid direct sequencing of both strands of long DNA molecules cloned in a plasmid. Nucleic Acids Research 11:5521–5540
    [Google Scholar]
  18. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  19. Hudson P. J., Mckern N. M., Power B. E., Azad A. A. 1986; Genomic structure of the large RNA segment of IBDV. Nucleic Acids Research 14:5001–5012
    [Google Scholar]
  20. Jackwood D. H., Saif Y. M. 1987; Antigenic diversity of infectious bursal disease viruses. Avian Diseases 31:766–770
    [Google Scholar]
  21. Jagadish M. N., Staton V. J., Hudson P. J., Azad A. A. 1988; Birnavirus precursor polyprotein is processed in Escherichia coli by its own virus-encoded polypeptide. Journal of Virology 62:1084–1087
    [Google Scholar]
  22. Kibenge F. S. B., Dhillon A. S., Russell R. G. 1988; Biochemistry and immunology of infectious bursal disease virus. Journal of General Virology 69:1757–1775
    [Google Scholar]
  23. Kozak M. 1987; An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Research 15:8125–8130
    [Google Scholar]
  24. Lange H., Müller H., Kaeufer I., Becht H. 1987; Pathogenic and structural properties of wild type infectious bursal disease virus (IBDV) and virus grown in vitro. Archives of Virology 92:187–196
    [Google Scholar]
  25. Mcferran J. B., Mcnulty M. S., Mckillop E. R., Conner T. J., Mccracken R. M., Collins D. S., Allan G. M. 1980; Isolation and serological studies with infectious bursal disease viruses from fowl, turkeys, and ducks: demonstration of a second serotype. Avian Pathology 9:395–404
    [Google Scholar]
  26. Müller H. K. 1988 Strukturelle und pathogeneEigenschaften der biedenSerotypensowieeirterReassortanten des Virus der infektiosen Bursitis (IBDV) Ph.D. Thesis Justus-Liebig-Universität, Giessen:
    [Google Scholar]
  27. Müller H., Becht H. 1982; Biosynthesis of virus-specific proteins in cells infected with IBDV: their significance as structural elements for infectious virus and incomplete particles. Journal of Virology 44:384–392
    [Google Scholar]
  28. Müller H., Scholtissek C., Becht H. 1979; The genome of infectious bursal disease virus consists of two segments of doublestranded RNA. Journal of Virology 31:584–589
    [Google Scholar]
  29. Müller H., Lange H., Becht H. 1986; Formation, characterization, and interfering capacity of a small plaque mutant and of incomplete virus particles of infectious bursal disease virus. Virus Research 4:297–309
    [Google Scholar]
  30. Nick H., Cursiefen D., Becht H. 1976; Structural and growth characteristics of infectious bursal disease virus. Journal of Virology 18:227–234
    [Google Scholar]
  31. Putney S. D., Benkovic S. J., Schimmel P. R. 1981; A DNA fragment with an alphaphosphorothioate nucleotide at one end is asymmetrically blocked from digestion by exonuclease III and can be replicated in vivo. Proceedings of the National Academy of Sciences, U.S.A 78:7350–7354
    [Google Scholar]
  32. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  33. Sedman S. A., Good P. J., Mertz J. E. 1989; Leader-encoded open reading frames modulate both the absolute and relative rates of synthesis of the virion proteins of simian virus 40. Journal of Virology 63:3884–3893
    [Google Scholar]
  34. Snyder D. B., Lana D. P., Cho B. R., Marquardt W. W. 1988; Group and strain-specific neutralisation sites of IBDV defined with monoclonal antibodies. Avian Diseases 32:527–534
    [Google Scholar]
  35. Spies U., Müller H., Becht H. 1987; Properties of RNA polymerase activity associated with infectious bursal disease virus and characterisation of its reaction products. Virus Research 8:127–140
    [Google Scholar]
  36. Staden R. 1986; The current status and portability of our sequence handling software. Nucleic Acids Research 14:217
    [Google Scholar]
  37. Tabor S., Richardson C. C. 1987; DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proceedings of the National Academy of Sciences, U.S.A 84:4767–4771
    [Google Scholar]
  38. Wood G. W., Drury S. E. N., Hourigan B. M. E., Muskett J. C., Thornton D. H., Fahey K. J. 1988; Antibody to the Australian 002-73 strain of infectious bursal disease virus protects against European IBD virus strains. Australian Veterinary Journal 65:94–95
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-71-6-1303
Loading
/content/journal/jgv/10.1099/0022-1317-71-6-1303
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error