1887

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Volume 71, Issue 6

Analysis of influenza A virus temperature-sensitive mutants with mutations in RNA segment 8 Free

Abstract

Temperature-sensitive (ts) mutants of influenza virus strain A/Udorn/72 (H3N2 subtype) with lesions in RNA segment 8 exhibited intrasegmental complementation, and were divided in two complementation groups (HI and H2) on MDCK cells. The nucleotide sequence of segment 8 was determined for three of these mutants. The HI strains, ICR1629 and SPC45, have a single amino acid substitution in the coding region of the non-structural protein NS1, whereas the H2 strain, ICR516, has a substitution in the NS2- coding region. With both NS1 ts mutants, the synthesis of two late proteins, the matrix protein (Ml) and haemagglutinin (HA), was greatly reduced and NS1 synthesis also decreased at 40 °C (non-permissive temperature) compared to that at 34 °C (permissive temperature). The synthesis of each virus-specific RNA was analysed using a quantitative hybridization method. However, at 40 °C, the levels of individual mRNAs including those for the late proteins, were almost the same as those at 34 °C, and attained the wild-type levels later in the infection (5 h postinfection) when the synthesis of the late proteins and the NS1 protein was severely reduced. The observations suggest that the NS 1 protein, which is a nuclear protein, is involved in some post-transcriptional processes in the synthesis of the late proteins and the NS1 protein.

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  • Accepted:
  • Published Online:
© Society for General Microbiology 1990
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1990-06-01
2024-04-26
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Analysis of influenza A virus temperature-sensitive mutants with mutations in RNA segment 8
J. Gen. Virol. 71, 1283 (1990); https://doi.org/10.1099/0022-1317-71-6-1283
/content/journal/jgv/10.1099/0022-1317-71-6-1283
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