1887

Abstract

Antibody-mediated enhancement of dengue virus replication is thought to be a mechanism contributing to the pathogenesis of dengue haemorrhagic fever and dengue shock syndrome. Enhancement is associated with antibodies to structural components of the virus. To circumvent the problem of immune enhancement, studies to identify protective antigens of dengue virus have involved non-structural proteins. Passive and active protection against lethal dengue virus infection in mice have been demonstrated with the nonstructural protein NS1. In this study, the dengue virus non-structural protein NS3 was examined in passive protection studies with monoclonal antibodies prepared against NS3 of dengue 1 virus (Hawaiian). Five monoclonal antibodies that were authenticated to be reactive to NS3 were used to immunize 13- to 14-day old mice intraperitoneally. Thereafter, the mice were challenged intracerebrally with 100 LD of neurotropic dengue 1 virus and the survival indices of the mice were calculated. Significant decreases in survival indices (< 0·05), indicating increases in survival times were observed with four of five monoclonal antibodies tested. Monoclonal antibodies to NS3 of dengue 1 virus are able to increase the survival time of mice challenged with a lethal dose of dengue 1 virus, although the mechanism remains to be defined.

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1990-03-01
2023-02-06
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References

  1. Bhamarapravati N. 1989; Live attenuated dengue vaccine development in Thailand. In Symposium on Dengue Fever Taipei, Taiwan:
    [Google Scholar]
  2. Bhamarapravati N., Yoksan S., Chanyaniyayothin T., Angsubphakorn S., Bunyaratvej A. 1987; Immunization with a live attenuated dengue 2 virus candidate vaccine (16681-PDK 53): clinical, immunological and biological responses in adult volunteers. Bulletin of the World Health Organization 65:189–195
    [Google Scholar]
  3. Clarke D. H., Casals J. 1958; Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  4. Deubel V., Kinney K. M., Trent D. W. 1988; Nucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 2 virus, Jamaica genotype: comparative analysis of the full length genome. Virology 165:234–244
    [Google Scholar]
  5. Dieckmann C. L., Tzagoloff A. 1985; Assembly of the mitochondrial membrane system. Journal of Biological Chemistry 260:1513–1530
    [Google Scholar]
  6. Earley E. M., Osterling M. C. 1985; Fusion of mouse-mouse cells to produce hybridomas secreting monoclonal antibodies. Journal of Tissue Culture Methods 9:141–146
    [Google Scholar]
  7. Falconar A. K. I., Young P. R. 1989; Functional analysis of a panel of monoclonal antibodies generated against the nonstructural glycoprotein, NS1 of dengue type 2 virus (PR1590). In 2nd International Symposium on Positive Strand RNA Viruses Vienna, Austria:
    [Google Scholar]
  8. Gould E. A., Buckley A., Barrett A. D. T., Cammack N. 1986; Neutralizing (54K) and non-neutralizing (54K and 48K) monoclonal antibodies against structural and non-structural yellow fever virus proteins confer immunity in mice. Journal of General Virology 67:591–595
    [Google Scholar]
  9. Halstead S. B. 1988; Pathogenesis of dengue: challenges to molecular biology. Science 239:476–481
    [Google Scholar]
  10. Henchal E. A., Henchal L. S., Schlesinger J. J. 1988; Synergistic interactions of anti-NSl monoclonal antibodies protect passively immunized mice from lethal challenge with dengue 2 virus. Journal of General Virology 69:2101–2107
    [Google Scholar]
  11. Hsiung G. D., Fong C. K. Y. 1982; Methods commonly used in virus isolation and detection. In Diagnostic Virology, 3rd edn. pp. 42–49 New Haven & London: Yale University Press;
    [Google Scholar]
  12. Kurane I., Bukowski J. F., Lai C.-J., Brinton M., Bray M., Falgout B., Zhao B., Ennis F. A. 1989; Dengue virus-specific human cytotoxic T lymphocytes (CTL). In 2nd International Symposium on Positive Strand RNA Viruses Vienna, Austria:
    [Google Scholar]
  13. Mackow E., Makino Y., Zhao B., Zhang Y.-M., Markoff L., Buckler-White A., Guiler M., Chanock R., Lai C.-J. 1987; The nucleotide sequence of dengue type 4 virus: analysis of genes coding for nonstructural proteins. Virology 159:217–228
    [Google Scholar]
  14. Preugschat F., Strauss J. H. 1989; In vivo processing of dengue 2 virus nonstructural proteins. In 2nd International Symposium on Positive Strand RNA Viruses Vienna, Austria:
    [Google Scholar]
  15. Reed L. J., Muench H. 1938; A simple method of estimating fifty percent endpoints. American Journal of Tropical Medicine and Hygiene 27:493–497
    [Google Scholar]
  16. Rice C. M., Lenches E. M., Eddy S. R., Shin S. J., Sheets R. L., Strauss J. H. 1985; Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. Science 229:726–733
    [Google Scholar]
  17. Rice C. M., Strauss E. G., Strauss J. H. 1986; Structure of the flavivirus genome. In Togaviridae and Flaviviridae pp. 279–326 Schlesinger M., Schlesinger S. Edited by New York: Plenum Press;
    [Google Scholar]
  18. Schlesinger J. J., Brandriss M. W., Walsh E. E. 1985; Protection against 17D yellow fever encephalitis in mice by passive transfer of monoclonal antibodies to the nonstructural glycoprotein gp48 and by active immunization with gp48. Journal of Immunology 135:2805–2809
    [Google Scholar]
  19. Schlesinger J. J., Brandriss M. W., Walsh E. E. 1987; Protection of mice against dengue 2 virus encephalitis by immunization with the dengue 2 virus non-structural glycoprotein NS1. Journal of General Virology 68:853–857
    [Google Scholar]
  20. Smith G. C. E., Westgarth D. R. 1957; The use of survival time in the analysis of neutralisation tests for serum antibody surveys. Journal of Hygiene 55:224–238
    [Google Scholar]
  21. Westaway E. G. 1977; Strategy of the flavivirus genome: evidence for multiple internal initiation of proteins specified by Kunjin virus in mammalian cells. Virology 80:320–335
    [Google Scholar]
  22. Zhang Y. M., Falgout B., Bray M., Dubois D. R., Eckels K. H., Chanock R. M., Lai C. J. 1989; Use of subunit proteins and recombinant vaccinia viruses expressing nonstructural protein NS1 for immunisation against dengue virus infection. In WHO Programme for Vaccine Development. Steering Committee on Dengue. Scientific Meeting: Development of Flavivirus Vaccines for Dengue and Japanese Encephalitis Vienna, Austria:
    [Google Scholar]
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