1887

Abstract

Feline immunodeficiency virus (FIV) grown in cat lymphocyte and thymocyte cultures was labelled with -[S]methionine or [H]glucosamine and virus-coded proteins were identified using immunoprecipitation. Polypeptides with apparent values of 15K, 24K, 43K, 50K, 120K and 160K were detected. An additional polypeptide of 10K was detected by Western blot analysis. The two highest species sometimes appeared as one band, of which only the 120K polypeptide was glycosylated. In the presence of tunicamycin gp120 was no longer detectable and a non-glycosylated precursor of 75K was found instead. Pulse-chase experiments suggested that the smaller polypeptides p24 and p15 are cleavage products of both p160 and p50. Western blot analysis using a rabbit serum directed against p26 of equine infectious anaemia virus (EIAV) and an anti-EIAV horse serum from a field case of infection revealed a cross-reactivity with p24 of FIV. Cat sera collected late after experimental FIV infection recognized p26 of EIAV, indicating a reciprocal cross-reactivity.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-3-739
1990-03-01
2024-03-01
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/3/JV0710030739.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-3-739&mimeType=html&fmt=ahah

References

  1. Goudsmit J., Houwers D. J., Smit L., Nauta I. M. 1986; LAV/HTLV III gaggene product p24 shares antigenic determinants with equine anaemia virus but not with visna virus or caprine arthritis encephalitis virus. Intervirology 26:169–173
    [Google Scholar]
  2. Hussain K. A., Issel C. J., Rwambo P. M., Arnizaut A. B., Ball J. M., Schnorr K. L., Montelaro R. C. 1988; Identification of gag precursor of equine infectious anaemia virus with monoclonal antibodies to the major viral core protein, p26. Journal of General Virology 69:1719–1724
    [Google Scholar]
  3. Jacks T., Power M. D., Masiarz F. R., Luciw P. A., Barr P. J., Varmus H. E. 1988; Characterization of ribosomal frameshifting in HIV-1 gag-pol expression. Nature; London: 331280–283
    [Google Scholar]
  4. Laskey R. A., Mills A. D. 1975; Quantitative detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  5. Olmsted R. A., Barnes A. K., Yamamoto J. K., Hirsch V. M., Purcell R. H., Johnson P. R. 1989; Molecular cloning of feline immunodeficiency virus. Proceedings of the National Academy of Sciences, U.S.A 86:2448–2452
    [Google Scholar]
  6. Pedersen N. C., Ho E. W., Brown M. L., Yamamoto J. K. 1987; Isolation of a T-lymphotropic virus from domestic cats with an immunodeficiency-like syndrome. Science 235:790–793
    [Google Scholar]
  7. Rottier P. J. M., Spaan W. J. M., Horzinek M. C., van der Zeijst B. A. M. 1981; Translation of three mouse hepatitis virus strain A subgenomic RNA’s in Xenopus laevis oocytes. Journal of Virology 38:20–26
    [Google Scholar]
  8. Saah A. J., Farzadegan H., Fox R., Nishanian P., Rinaldo C. R., Phair J. P., Fahey J. L., Lee T.-H., Polk B.P. The Multicenter Aids Cohort Study 1987; Detection of early antibodies in human immunodeficiency virus infection by enzyme-linked immunosorbent assay, Western blot, and radioimmunopreci-pitation. Journal of Clinical Microbiology 25:1605–1610
    [Google Scholar]
  9. Steinman R., Dombrowski J., O’Connor T., Montelaro R. C., Tonelli Q., Lawrence K., Seymour C., Goodness J., Pedersen N., Andersen P. R. 1990; Biochemical and immunological characterization of the major structural proteins of feline immunodeficiency virus. Journal of General Virology 71:701–706
    [Google Scholar]
  10. Stephens R. M., Casey J. W., Rice N. R. 1986; Equine infectious anemia virus gag and pol genes: relatedness to visna and AIDS virus. Science 231:589–594
    [Google Scholar]
  11. Talbott R. L., Sparger E. E., Lovelace K. M., Fitch W. M., Pedersen N. C., Luciw P. A., Elder J. H. 1989; Nucleotide sequence and genomic organization of feline immunodeficiency virus. Proceedings of the National Academy of Sciences, U.S.A 86:5743–5747
    [Google Scholar]
  12. Van der Maaten M. J., Boothe A. D., Seger C. L. 1972; Isolation of a virus from cattle with persistent lymphocytosis. Journal of the National Cancer Institute 49:1649–1657
    [Google Scholar]
  13. Veronese F. D., Devico A. L., Copeland T. D., Oroszlan S., Gallo R. C., Sarngadharan M. G. 1985; Characterization of gp41 as the transmembrane protein coded by the HTLV-III/LAV envelope gene. Science 229:1402–1405
    [Google Scholar]
  14. Yamamoto J. K., Sparger E., Ho E. W., Andersen P. R., O’Connor T. P., Mandell C. P., Lowenstine L., Munn R., Pedersen N. C. 1988; Pathogenesis studies of experimentally induced feline immunodeficiency virus infection. American Journal of Veterinary Research 49:1246–1258
    [Google Scholar]
  15. Zhou Y., Ederveen J., Egberink H. F., Pensaert M., Horzinek M. C. 1988; Porcine epidemic diarrhea virus (CV777) and feline infectious peritonitis (FIPV) are antigenically related. Archives of Virology 102:63–71
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-3-739
Loading
/content/journal/jgv/10.1099/0022-1317-71-3-739
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error