1887

Abstract

protoplasts inoculated with raspberry ringspot virus accumulated top component (T), middle component (M) and bottom component (B) particles progressively from about 20 h post-infection (p.i.) until at least 73 h p.i.; the ratio between the amounts of T and B particles changed little during the multiplication cycle of the virus. In contrast, in raspberry ringspot virus-infected protoplasts although the accumulation of T particles continued up to at least 91 h p.i., the accumulation of B and M particles decreased greatly or ceased after about 60 h p.i. In either species of protoplast there was no evidence that T particles arose from B or M particles although some physical treatments did cause an apparent loss of nucleic acid from B and M particles.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-3-713
1990-03-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/3/JV0710030713.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-3-713&mimeType=html&fmt=ahah

References

  1. Barbara D. J., Clark M. F. 1982; A simple indirect ELISA using F(ab′)2 fragments of immunoglobulin. Journal of General Virology 58:315–322
    [Google Scholar]
  2. Barker H. 1980 Studies on the behaviour of raspberry ringspot virus in plant leaves and protoplasts PH.D. thesis University of Dundee:
    [Google Scholar]
  3. Barker H., Harrison B. D. 1977; Infection of tobacco mesophyll protoplasts with raspberry ringspot virus alone and together with tobacco rattle virus. Journal of General Virology 35:125–133
    [Google Scholar]
  4. Barker H., Harrison B. D. 1982; Infection of potato mesophyll protoplasts with five plant viruses. Plant Celt Reports 1:247–249
    [Google Scholar]
  5. Chu P. W. G., Boccardo G., Francki R. I. B. 1981; Requirement of a genome-associated protein of tobacco ringspot virus for infectivity but not for in vitro translation. Virology 109:428–430
    [Google Scholar]
  6. Cornelissen B. J. C., Linthorst H. J. M., Brederode F. T., Bol J. F. 1986; Analysis of the genome structure of tobacco rattle virus strain PSG. Nucleic Acids Research 14:2157–2167
    [Google Scholar]
  7. Francki R. I. B., Matthews R. E. F. 1962; Some effects of 2-thiouracil on the multiplication of turnip yellow mosaic virus. Virology 17:367–380
    [Google Scholar]
  8. Harrison B. D. 1958; Further studies on raspberry ringspot and tomato black ring, soil-borne viruses that affect raspberry. Annals of Applied Biology 46:571–584
    [Google Scholar]
  9. Harrison B. D., Murant A. F., Mayo M. A. 1972; Two properties of raspberry ringspot virus determined by its smaller RNA. Journal of General Virology 17:137–141
    [Google Scholar]
  10. Jones A. T., Mayo M. A., Henderson S. M. 1985; Biological and biochemical properties of an isolate of cherry rasp leaf virus from red raspberry. Annals of Applied Biology 106:101–110
    [Google Scholar]
  11. Koenig I., Fritsch C. 1982; A protein linked at the 5′ end of satellite and genomic tomato black ring virus RNAs: study of in vitro translation after protease treatment. Journal of General Virology 60:343–353
    [Google Scholar]
  12. Kubo S., Harrison B. D., Barker H. 1975; Defined conditions for growth of tobacco plants as sources of protoplasts for virus infection. Journal of General Virology 28:255–257
    [Google Scholar]
  13. Mayo M. A. 1987; A comparison of the translation strategies used by bipartite genome, RNA plant viruses. In The Molecular Biology of the Positive Strand RNA Viruses pp. 177–205 Rowlands D. J., Mayo M. A., Mahy B. W. J. Edited by London: Academic Press;
    [Google Scholar]
  14. Mayo M. A., Barker H. 1983; Defective multiplication of a pseudorecombinant of tomato black ring virus in tobacco protoplasts. Journal of General Virology 64:649–656
    [Google Scholar]
  15. Mayo M. A., Harrison B. D., Murant A. F., Barker H. 1973; Cross-linking of RNA induced by ultraviolet irradiation of particles of raspberry ringspot virus. Journal of General Virology 19:155–159
    [Google Scholar]
  16. Mayo M. A., Barker H., Harrison B. D. 1982; Specificity and properties of the genome-linked proteins of nepoviruses. Journal of General Virology 59:149–162
    [Google Scholar]
  17. Murant A. F., Mayo M. A., Harrison B. D., Goold R. A. 1972; Properties of virus and RNA components of raspberry ringspot virus. Journal of General Virology 16:327–338
    [Google Scholar]
  18. Quacquarelli A., Gallitelli D., Savino V., Martelli G. P. 1976; Properties of grapevine fanleaf virus. Journal of General Virology 32:349–360
    [Google Scholar]
  19. Ralph R. K., Matthews R. E. F., Matus A. I. 1965; Effects of 2-thiouracil on the formation of double-stranded plant viral ribonucleic acid. Biochimica et biophysica acta 108:53–66
    [Google Scholar]
  20. Rezaian M. A., Francki R. I. B. 1973; Replication of tobacco ringspot virus. I, Detection of a low molecular weight doublestranded RNA from infected plants. Virology 56:238–249
    [Google Scholar]
  21. Rottier P. J. M., Rezelman G., Van Kammen A. 1979; The inhibition of cowpea mosaic virus replication by actinomycin D. Virology 92:299–309
    [Google Scholar]
  22. Schneider I. R., Diener T. O. 1966; The correlation between the proportions of virus-related products and the infectious component during the synthesis of tobacco ringspot virus. Virology 29:92–99
    [Google Scholar]
  23. Widholm J. M. 1972; The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technology 47:189–194
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-3-713
Loading
/content/journal/jgv/10.1099/0022-1317-71-3-713
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error