1887

Abstract

The diabetogenic D variant of encephalomyocarditis virus (EMC-D) was previously shown to differ from the non-diabetogenic B variant (EMC-B) by 14 nucleotides out of 7829 bases. Similar approaches with a new nondiabetogenic variant, EMC-DVi, obtained by plaque purification of the EMC-D variant stock pool, enabled us to narrow down further the possible genomic area responsible for the diabetogenicity of EMC virus. EMC-DV ] does not induce interferon differing from the highly interferon-inducing EMC-B. The complete nucleotide sequence of EMC-DY, was determined by RNA-dependent DNA sequencing and cDNA sequencing. The genomic size and organization of EMC-EMC-DV are similar to those of EMC-D and EMC-B, with a long open reading frame encoding a polyprotein of 2292 amino acids. Comparative analyses of sequence information as well as biological activities of EMC-EMC-DV with EMC-D and EMC-B suggest that (i) the diabetogenicity is apparently distinct from the ability to induce interferon, which is probably due to the single U base insertion at position 765 in EMC-B, and (ii) the diabetogenicity of EMC virus is most probably controlled by one or both of two amino acids, Phe 16 (on the leader peptide) and Ala 776 (152nd amino acid on the VP1) on the polyprotein.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-3-639
1990-03-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/3/JV0710030639.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-3-639&mimeType=html&fmt=ahah

References

  1. Bae Y. S., Eun H. M., Yoon J. W. 1989; Genomic differences between the diabetogenic and nondiabetogenic encephalomyocardi- tis virus. Virology 170:282–287
    [Google Scholar]
  2. Bischoff J. F., Samuel C. E. 1989; Mechanism of interferon action; activation of the human Pl/elF-2d protein kinase by individual reovirus S-class mRNAs: SI mRNA is a potent activator relative to S4 mRNA. Virology 172:106–115
    [Google Scholar]
  3. Cohen S. H., Bolton V., Jordan G. W. 1983; Relationship of interferon-inducing particle phenotype to encephalomyocarditis virus-induced diabetes mellitus. Infection and Immunity 42:605–611
    [Google Scholar]
  4. Craighead J. E., Mclane M. F. 1968; Diabetes mellitus: induction in mice by encephalomyocarditis virus. Science 162:913–914
    [Google Scholar]
  5. Eun H. M., Bae Y. S., Yoon J. W. 1988; Amino acid differences in capsid protein, VP1, between diabetogenic and nondiabetogenic variants of encephalomyocarditis virus. Virology 163:369–373
    [Google Scholar]
  6. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  7. Jordan G. W., Cohen S. H. 1987; Encephalomyocarditis virus- induced diabetes mellitus in mice: model of viral pathogenesis. Reviews of Infectious Diseases 9:917–924
    [Google Scholar]
  8. Kaptur P. E., Thomas D. C., Giron D. J. 1989; Differing attachment of diabetogenic and nondiabetogenic variants of encephalomyocarditis virus to beta-cells. Diabetes 38:1103–1108
    [Google Scholar]
  9. Lewis J. A. 1987; Biological assay for interferon. In Lymphokines and Interferons pp 73–87 Clemens M. J., Morris A. G., Gearing A. J. H. Edited by Oxford: IRL Press;
    [Google Scholar]
  10. Luo M., Vriend G., Kamer G., Minor I., Arnold E., Rossmann M. G., Boege U., Scraba D. G., Duke G. M., Palmenberg A. C. 1987; The atomic structure of mengovirus at 3·0 Å resolution. Science 235:182–191
    [Google Scholar]
  11. Marcus P. I., Garcione C. 1989; Interferon induction by viruses. XIX. Vesicular stomatitis virus-New Jersey: high multiplicity passage generates interferon-inducing, defective interfering particles. Virology 171:630–633
    [Google Scholar]
  12. Okayama H., Berg P. 1982; High efficiency clonings of full-length cDNA. Molecular and Cellular Biology 2:161–170
    [Google Scholar]
  13. Palmenberg A. C., Kirby E. M., Janda M. R., Drake N. L., Duke G. M., Potratz K. F., Collett M. S. 1984; The nucleotide and deduced amino acid sequences of the encephalomyo-carditis viral polyprotein coding region. Nucleic Acids Research 12:2969–2985
    [Google Scholar]
  14. Rossmann M. G., Palmenberg A. C. 1988; Conservation of the putative receptor attachment site in picomaviruses. Virology 164:373–382
    [Google Scholar]
  15. Rossmann M. G., Arnold E., Erickson J. W., Frankenberger E. A., Griffith J. P., Hecht H. J., Johnson J. E., Kamer G., Luo M., Mosser A. G., Lueckert R. R., Sherry B., Vriend G. 1985; Structure of a human common cold virus and functional relationship to other picomaviruses. Nature; London: 317145–153
    [Google Scholar]
  16. Sanger F., Carlson A. R., Barrerl B. G., Smith A. J. H., Roe B. A. 1980; Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  17. Yoon J. W., Notkins A. L. 1983; Virus-induced diabetes in mice. Metabolism 32: Suppl 37–40
    [Google Scholar]
  18. Yoon J. W., Onodera T., Notkins A. L. 1977; Virus-induced diabetes mellitus: VIII. Passage of encephalomyocarditis vims and severity of diabetes in susceptible and resistant strains of mice. Journal of General Virology 37:225–232
    [Google Scholar]
  19. Yoon J. W., Mcclintock P. R., Onodera T., Notkins A. L. 1980; Virus-induced diabetes mellitus. XVIII. Inhibition by a nondiabetogenic variant of encephalomyocarditis vims. Journal of Experimental Medicine 152:878–892
    [Google Scholar]
  20. Yoon J. W., Cha C. Y., Jordan G. W. 1983; Role of interferon in virus-induced diabetes in mice. Journal of Infectious Diseases 147:155–159
    [Google Scholar]
  21. Yoon J. W., Wong A. K. C., Bae Y. S., Eun H. M. 1988; An apparent deletion of an oligonucleotide directed by RN A fingerprint in the nondiabetogenic B variant of encephalomyocarditis vims is caused by a point mutation. Journal of Virology 62:637–640
    [Google Scholar]
  22. Zuker M., Stiegler P. 1981; Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information. Nucleic Acids Research 9:133–148
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-3-639
Loading
/content/journal/jgv/10.1099/0022-1317-71-3-639
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error