1887

Abstract

The sequences of the P mRNA and protein of strain 18537 of antigenic subgroup B of human respiratory syncytial virus were determined by sequencing cloned cDNAs of intracellular mRNA. Comparison with the corresponding sequences of the A2 strain of subgroup A showed that there was extensive sequence identity at both the nucleotide (80% identity) and amino acid (90% identity) levels. The P proteins contained a single divergent region (52% amino acid identity) flanked by highly conserved domains (96% identity). The previously observed differences in electrophoretic mobilities between the P proteins of subgroup A strains and certain subgroup B strains could not be attributed to differences in the polypeptide of the primary translation product.

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1990-02-01
2022-07-07
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References

  1. Åkerlind B., Norrby E., Örvell C., Mufson M. A. 1988; Respiratory syncytial virus: heterogeneity of subgroup B strains. Journal of General Virology 69:2145–2154
    [Google Scholar]
  2. Anderson L. J., Hierholzer J. C., Tsou C., Hendry R. M., Fernie B. F., Stone Y., Mcintosh K. 1985; Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. Journal of Infectious Diseases 151:626–633
    [Google Scholar]
  3. Baybutt H. N., Pringle C. R. 1987; Molecular cloning and sequencing of the F and 22K membrane protein genes of the RSS-2 strain of respiratory syncytial virus. Journal of General Virology 68:2789–2796
    [Google Scholar]
  4. Cattaneo R., Kaelin K., Baczko K., Billeter M. A. 1989; Measles virus editing provides an additional cysteine-rich protein. Cell 56:759–764
    [Google Scholar]
  5. Chinchar V. G., Portner A. 1981; Functions of Sendai virus nucleocapsid polypeptides: enzymatic activities in nucleocapsids following cleavage of polypeptide P by Staphylococcus aureus protease V8. Virology 109:59–71
    [Google Scholar]
  6. Coates H. V., Alling D. W., Chanock R. M. 1966; An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. American Journal of Epidemiology 83:299–313
    [Google Scholar]
  7. Collins P. L., Wertz G. W. 1985; Nucleotide sequences of the IB and 1C nonstructural protein mRNAs of human respiratory syncytial virus. Virology 143:442–451
    [Google Scholar]
  8. Collins P. L., Huang Y. T., Wertz G. W. 1984; Identification of a tenth mRNA of respiratory syncytial virus and assignment of polypeptides to the 10 viral genes. Journal of Virology 49:572–578
    [Google Scholar]
  9. Collins P. L., Anderson K., Langer S. J., Wertz G. W. 1985; Correct sequence for the major nucleocapsid protein mRNA of respiratory syncytial virus. Virology 146:69–77
    [Google Scholar]
  10. Collins P. L., Dickens L. E., Buckler-White A., Olmsted R. A., Spriggs M. K., Camargo E., Coelingh K. V. W. 1986; Nucleotide sequences for the gene junctions of human respiratory syncytial virus reveal distinctive features of intergenic structure and gene order. Proceedings of the National Academy of Sciences, U.S.A 83:4594–4598
    [Google Scholar]
  11. Despande K. L., Portner A. 1985; Monoclonal antibodies to the P protein of Sendai virus define its structure and role in transcription. Virology 140:125–134
    [Google Scholar]
  12. Gimenez H. B., Hardman N., Keir H. M., Cash P. 1986; Antigenic variation between human respiratory syncytial virus isolates. Journal of General Virology 67:863–870
    [Google Scholar]
  13. Hendry R. M., Talis A. L., Godfrey E., Anderson L. J., Fernie B. F., Mcintosh K. 1986; Concurrent circulation of antigeni- cally distinct strains of respiratory syncytial virus during community outbreaks. Journal of Infectious Diseases 153:291–297
    [Google Scholar]
  14. Hsu C. -H., Kingsbury D. W. 1982; Topography of phosphate residues in Sendai virus proteins. Virology 120:225–234
    [Google Scholar]
  15. Huang Y. T., Collins P. L., Wertz G. W. 1985; Characterization of the 10 proteins of human respiratory syncytial virus: identification of a fourth envelope-associated protein. Virus Research 2:157–173
    [Google Scholar]
  16. Johnson P. R., Collins P. L. 1988a; The fusion glycoproteins of human respiratory syncytial virus of subgroups A and B: sequence conservation provides a structural basis for antigenic relatedness. Journal of General Virology 69:2623–2628
    [Google Scholar]
  17. Johnson P. R., Collins P. L. 1988b; The A and B subgroups of human respiratory syncytial virus: comparison of intergenic and gene-overlap sequences. Journal of General Virology 69:2901–2906
    [Google Scholar]
  18. Johnson P. R., Collins P. L. 1989; The IB (NS2), 1C (NS1) and N proteins of human respiratory syncytial virus (RSV) of antigenic subgroups A and B: sequence conservation and divergence within RSV genomic RNA. Journal of General Virology 70:1539–1547
    [Google Scholar]
  19. Johnson P. R., Olmsted R. A., Prince G. A., Murphy B. R., Alling D. W., Walsh E. E., Collins P. L. 1987a; Antigenic relatedness between glycoproteins of human respiratory syncytial virus subgroups A and B: evaluation of the contributions of the F and G glycoproteins to immunity. Journal of Virology 61:3163–3166
    [Google Scholar]
  20. Johnson P. R., Spriggs M. K., Olmsted R. A., Collins P. L. 1987b; The G glycoproteins of human respiratory syncytial virus subgroups A and B: extensive sequence divergence between antigenically related proteins. Proceedings of the National Academy of Sciences, U.S.A. 84:5625–5629
    [Google Scholar]
  21. Lambden P. R. 1985; Nucleotide sequence of the respiratory syncytial virus phosphoprotein gene. Journal of General Virology 66:1607–1612
    [Google Scholar]
  22. Lopez J. A., Villanueva N., Melero J. A, Portela A. 1988; Nucleotide sequence of the fusion and phosphoprotein genes of human respiratory syncytial (RS) virus Long strain: evidence of subtype genetic heterogeneity. Virus Research 10:249–262
    [Google Scholar]
  23. Mcintosh K. M., Chanock R. M. 1985; Respiratory syncytial virus. In Virology pp. 1285–1304 Fields B. N. Edited by New York: Raven Press;
    [Google Scholar]
  24. Morgan L. A., Routledge E. G., Willcocks M. M., Samson A. C. R., Scott R., Toms G. L. 1987; Strain variation of respiratory syncytial virus. Journal of General Virology 68:2781–2788
    [Google Scholar]
  25. Mufson M. A., ÖRvell C., Rafnar B., Norrby E. 1985; Two distinct subtypes of human respiratory syncytial virus. Journal of General Virology 66:2111–2124
    [Google Scholar]
  26. Norrby E., Mufson M. A., Sheshberadaran H. 1986; Structural differences between subtype A and B strains of respiratory syncytial virus. Journal of General Virology 67:2721–2729
    [Google Scholar]
  27. Olmsted R. A., Collins P. L. 1989; The 1A protein ot respiratory syncytial virus is an integral membrane protein present as multiple, structurally distinct species. Journal of Virology 63:2019–2129
    [Google Scholar]
  28. Pringle C. R., Shirodaria P. V., Gimenez H. B., Levine S. 1981; Antigen and polypeptide synthesis by temperature-sensitive mutants of respiratory syncytial virus. Journal of General Virology 54:173–183
    [Google Scholar]
  29. Satake M., Elango N., Venkatesan S. 1984; Sequence analysis of the respiratory syncytial virus phosphoprotein gene. Journal of Virology 52:991–994
    [Google Scholar]
  30. Spriggs M. K., Collins P. L. 1986; Sequence analysis of the P and C protein genes of human parainfluenza virus type 3: patterns of amino acid sequence homology among paramyxovirus proteins. Journal of General Virology 67:2705–2719
    [Google Scholar]
  31. Storch G. A., Park C. S., Dohner D. E. 1989; RNA fingerprinting of respiratory syncytial virus using ribonuclease protection: application to molecular epidemiology. Journal of Clinical Investigation 83:1894–1902
    [Google Scholar]
  32. Stott E. J., Taylor G., Ball L. A., Anderson K., Young K. K. Y., King A. M. Q., Wertz G. W. 1987; Immune and histopathological responses in animals vaccinated with recombinant vaccinia viruses that express individual genes of human respiratory syncytial virus. Journal of Virology 61:3855–3861
    [Google Scholar]
  33. Thomas S. M., Lamb R. A., Paterson R. G. 1988; Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5. Cell 54:891–902
    [Google Scholar]
  34. Vidal S., Curran J., Örvell C., Kolakofsky D. 1988; Mapping of monoclonal antibodies to the Sendai virus P protein and the location of its phosphates. Journal of Virology 62:2200–2203
    [Google Scholar]
  35. Walsh E. E., Brandriss M. W., Schlesinger J. J. 1987; Immunological differences between the envelope glycoproteins of two strains of human respiratory syncytial virus. Journal of General Virology 68:2169–2176
    [Google Scholar]
  36. Wilbur W. J., Lipman D. J. 1983; Rapid similarity searches of nucleic acid and protein data bases. Proceedings of the National Academy of Sciences, U S A 80:726–730
    [Google Scholar]
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