1887

Abstract

Transcripts from two immediate early (IE) genes have been identified in cells infected with the gammaherpes- virus, herpesvirus saimiri. One is a 1·3 kb RNA transcribed from the dIII-G fragment of virus DNA (IE-G), the other is a 1·6 kb RNA from the gene for the IE 52K phosphoprotein. Labelled oligonucleotide probes specific for each of these RNAs have been used in hybridization experiments to compare their expression in individual cells in infected populations. In the presence of cycloheximide, the IE-G RNA accumulates synchronously throughout the population of infected cells and prior to the asynchronous accumulation of RNA from the gene for the IE 52K protein in the same population of cells. This heterogeneity in the timing of expression of RNA from the IE 52K gene is paralleled by the asynchronous accumulation of the protein product. We conclude that transcription of the IE-G RNA is independent of expression of the IE 52K gene and that expression of the 52K gene requires (or is prevented by) factors which do not affect accumulation of the RNA from the IE-G gene.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-12-3005
1990-12-01
2021-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/12/JV0710123005.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-12-3005&mimeType=html&fmt=ahah

References

  1. Akrigg A., Wilkinson G. W. G., Oram J. D. 1985; The structure of the major immediate early gene of human cytomegalovirus strain AD 169. Virus Research 2:107–121
    [Google Scholar]
  2. Baer R., Bankier A. T., Biggin M., Deininger P. L., Farrell P. J., Gibson T. J., Hatfull G., Hudson G. D., Satchwell S. C., Seguin C., Tufnell P. S., Barrell B. G. 1984; DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature; London: 310207–211
    [Google Scholar]
  3. Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., Hutchison C. A.III Kouzarides T., Martignetti J. A., Preddie E., Satchwell S. C., Tomlinson P., Weston K. M., Barrell B. G. 1990; An analysis of the protein coding content of the sequence of human cytomegalovirus strain AD 169. In Current Topics in Microbiology and Immunology 154 pp 129–173 Mcdougall J. K. Edited by Berlin & Heidelberg: Springer-Verlag;
    [Google Scholar]
  4. Davison A. J., Scott J. E. 1986; The complete DNA sequence of varicella-zoster virus. Journal of General Virology 67:1759–1816
    [Google Scholar]
  5. Davison A. J., Taylor P. 1987; Genetic relations between varicella-zoster virus and Epstein-Barr virus. Journal of General Virology 68:1067–1079
    [Google Scholar]
  6. Honess R. W. 1984; Herpes simplex and ‘the herpes complex’: diverse observations and a unifying hypothesis. Journal of General Virology 65:2077–2107
    [Google Scholar]
  7. Honess R. W., Roizman B. 1974; Regulation of herpes virus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins. Journal of Virology 14:8–19
    [Google Scholar]
  8. Kieff E., Leibowitz D. 1990; Epstein-Barr virus and its replication. In Virology, 2nd edn.. pp 1889–1920 Fields B. N. Edited by New York: Raven Press;
    [Google Scholar]
  9. McGeoch D. J., Dalrymple M. A., Davison A. J., Dolan A., Frame M, McNab D., Perry L. J., Scott J. E., Taylor P. 1988; The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. Journal of General Virology 69:1531–1574
    [Google Scholar]
  10. Nicholas J., Gompels U. A., Craxton M. A., Honess R. W. 1988; Conservation of sequence and function between the products of the 52-kilodalton immediate-early gene of herpesvirus saimiri and the BMLF-1 encoded transcription at effector (EB2) of Epstein-Barr virus. Journal of Virology 62:3250–3257
    [Google Scholar]
  11. Nicholas J., Smith E. P., Coles L., Honess R. 1990; Gene expression in cells infected with gamma herpesvirus saimiri: properties of transcripts from two immediate-early genes. Virology in press
    [Google Scholar]
  12. Randall R. E., Newman C., Honess R. W. 1984a; A single major immediate-early virus gene product is synthesised in cells productively infected with herpesvirus saimiri. Journal of Virology 65:1215–1219
    [Google Scholar]
  13. Randall R. E., Newman C., Honess R. W. 1984b; Isolation and characterization of monoclonal antibodies to structural and nonstructural herpesvirus saimiri proteins. Journal of Virology 65:872–883
    [Google Scholar]
  14. Randall R. E., Newman C., Honess R. W. 1985; Asynchronous expression of the immediate-early protein of herpesvirus saimiri in populations of productively infected cells. Journal of General Virology 66:2199–2213
    [Google Scholar]
  15. Roizman B., Sears A. E. 1990; Herpes simplex viruses and their replication. In Virology, 2nd edn.. pp 1795–1841 Fields B. N. Edited by New York: Raven Press;
    [Google Scholar]
  16. Stenberg R. M., Thomsen D. R., Stinski M. F. 1984; Structural analysis of the major immediate early gene of human cytomegalovirus. Journal of Virology 49:190–199
    [Google Scholar]
  17. Stinski M. F. 1990; Cytomegalovirus and its replication. In Virology, 2nd edn.. pp 1959–1980 Fields B. N. Edited by New York: Raven Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-12-3005
Loading
/content/journal/jgv/10.1099/0022-1317-71-12-3005
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error