1887

Abstract

The distribution of three adenovirus-encoded DNA replication proteins in the nucleus of human 293 cells was studied by immunogold electron microscopy. The infected nuclei contained four morphologically distinct inclusions. They were highly electron-dense granules (type I), compact fibrogranular masses of medium electron density (type II), filamentous masses of low electron density (type III) and large polygonal crystals (type IV). In immunogold labelling studies, antibodies to the adenovirus single-stranded DNA-binding protein (DBP) and antibodies to single-stranded DNA showed extensive binding to the type III inclusions. The antibodies to the adenovirus DNA polymerase (AdPol) and terminal protein (TP) predominantly labelled type II inclusions. Double immunogold labelling studies detected low levels of AdPol and TP in type III inclusions and DBP in type II inclusions. The selective distribution of DNA replication proteins suggests that the type II and III inclusions represent two functionally different entities that may be involved in two different aspects of adenovirus DNA replication, i.e. chain initiation and elongation.

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1990-12-01
2022-09-25
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References

  1. Beer M., Zobel R. C. 1961; Electron stains II. Electron microscopic studies on the visibility of stained DNA molecules. Journal of Molecular Biology 3:717–726
    [Google Scholar]
  2. Boulanger P. A., Torpier G., Biserte G. 1970; Investigation on intranuclear paracrystalline inclusions induced by adenovirus 5 in KB cells. Journal of General Virology 6:329–332
    [Google Scholar]
  3. Challberg M. D., Kelly T. J. 1989; Animal virus DNA replication. Annual Review of Biochemistry 58:671–717
    [Google Scholar]
  4. Challberg M. D., Desiderio S. V., Kelly T. J. JR 1980; Adenovirus DNA replication in vitro: characterization of a protein covalently linked to nascent DNA strands. Proceedings of the National Academy of Sciences, U.S.A 77:5105–5109
    [Google Scholar]
  5. Challberg M. D., Ostrove J. M., Kelly T. J. JR 1982; Initiation of adenovirus DNA replication: detection of covalent complexes between nucleotides and the 80-kilodalton terminal protein. Journal of Virology 41:265–270
    [Google Scholar]
  6. Friefeld B. R., Krevolin M. D., Horwitz M. S. 1983; Effectsof the adenovirus H5tsl25 and H5tsl07 DNA binding proteins on DNA replication in vitro . Virology 124:380–389
    [Google Scholar]
  7. Friefeld B. R., Lichy J. H., Field J., Gronostajski R. M., Guggenheimer M. D., Krevolin M. D., Nagata K., Hurwitz J., Horwitz M. S. 1984; The in vitro replication of adenovirus DNA. Current Topics in Microbiology and Immunology 110:221–255
    [Google Scholar]
  8. Friefeld B. R., Korn R., De Long P. J., Sninsky J. J., Horwitz M. S. 1985; The 140 kDa adenovirus DNA polymerase is recognized by antibodies to Escherichia coli-synthesized determinants predicted from an open reading frame on the adenovirus genome. Proceedings of the National Academy of Sciences, U.S.A 82:2652–2656
    [Google Scholar]
  9. Graham F. L., Smiley J., Russell W. C., Nairn R. 1977; Characterization of a human cell line transformed by DNA from human adenovirus type 5. Journal of General Virology 36:59–72
    [Google Scholar]
  10. Kalnins V. I., Stich H. F., Yohn D. S. 1966; Electron microscopic localization of virus-associated antigens in human amnion cells (AV-3) infected with human adenovirus type 12. Virology 28:751–754
    [Google Scholar]
  11. Kaplan L. M., Ariga H., Hurwitz J., Horwitz M. S. 1979; Complementation of the temperature-sensitive defect in H5tsl25 adenovirus DNA replication in vitro . Proceedings of the National Academy of Sciences, U.S.A 76:5534–5538
    [Google Scholar]
  12. Kelly T. J. JR 1984; Adenovirus DNA replication. In The Adenoviruses 19 pp. 271–308 Ginsberg H. S. Edited by New York: Plenum Press;
    [Google Scholar]
  13. Klessig D. F., Rice S. A., Cleghon V., Brough D. E., Williams J. F., Voelkerding K. 1986; Studies on the adenovirus DNA-binding protein. In DNA Tumor Viruses: Control of Gene Expression and Replication pp. 485–496 Botchan M., Grodzicker T., Sharp P. A. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Levinthal J. D., Cerottini J. C., Ahmad-zadeh C., Wicker R. 1967; The detection of intracellular adenovirus 12 antigens by indirect immunoferritin. International Journal of Cancer 2:143–152
    [Google Scholar]
  15. Martinez-palomo A., Le Buis J., Bernhard W. 1967; Electron microscopy of adenovirus 12 replication. I. Fine structural changes in the nucleus of infected KB cells. Journal of Virology 1:817–829
    [Google Scholar]
  16. Marusyk R., Norrby E., Marusyk H. 1972; The relationship of adenovirus-induced paracrystalline structures to the virus core protein(s). Journal of General Virology 14:261–270
    [Google Scholar]
  17. Nagata K., Guggenheimer R. A., Enomoto T., Lichy J. H., Hurwitz J. 1982; Adenovirus DNA replication in vitro: identification of a host factor that stimulates synthesis of the preterminal protein-dCMP complex. Proceedings of the National Academy of Sciences, U.S.A 79:6438–6442
    [Google Scholar]
  18. Nagata K., Guggenheimer R. A., Hurwitz J. 1983; Adenovirus DNA replication in vitro: synthesis of full length DNA with purified-proteins. Proceedings of the National Academy of Sciences, U.S.A. 80:4266–4270
    [Google Scholar]
  19. Neale G. A. M., Kitchingman G. R. 1990; Conserved region 3 of the adenovirus type 5 DNA-binding protein is important for interaction with single-stranded DNA. Journal of Virology 64:630–638
    [Google Scholar]
  20. Reich N. C., Sarnow P., Duprey E., Levine A. J. 1983; Monoclonal antibodies which recognize native and denatured forms of the adenovirus DNA-binding protein. Virology 128:480–484
    [Google Scholar]
  21. Spector D. L. 1990; Higher order nuclear organization: threedimensional distribution of small nuclear ribonucleoprotein particles. Proceedings of the National Academy of Sciences, U.S.A 87:147–151
    [Google Scholar]
  22. Stunnenberg H. G., Lange H., Philipson L., Van Miltenburg R. T., Van Der Vliet P. C. 1988; High expression of functional adenovirus DNA polymerase and precursor terminal protein using recombinant vaccinia virus. Nucleic Acids Research 16:2431–2444
    [Google Scholar]
  23. Sugawara K., Gilead Z., Wold W. S. M., Green M. 1977; Immunofluorescence study of the adenovirus type 2 single-stranded DNA binding protein in infected and transformed cells. Journal of Virology 22:527–539
    [Google Scholar]
  24. Sussenbach J. S., Van Der Vliet P. C. 1983; The mechanism of adenovirus DNA replication and the characterization of replication proteins. Current Topics in Microbiology and Immunology 109:55–73
    [Google Scholar]
  25. Voelkerding K., Klessig D. F. 1986; Identification of two nuclear subclasses of the adenovirus type 5-encoded DNA-binding protein. Journal of Virology 60:353–362
    [Google Scholar]
  26. Weber J., Liao S. -K. 1969; Light and electron microscopy of virus- associated intranuclear paracrystals in cultured cells infected with type 2,4,6 and 18 human adenoviruses. Canadian Journal of Microbiology 15:841
    [Google Scholar]
  27. Yamamoto T. 1969; Sequential study of the development of infectious canine laryngotracheitis adenovirus. Journal of General Virology 4:397–401
    [Google Scholar]
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