1887

Abstract

Infectious hypodermal and haematopoietic necrosis (IHHN) is one of the most important viral diseases of cultured penaeid shrimps and is potentially a limiting factor in the development of farming projects for some species of these shrimps. Although the IHHN agent was recognized early as being viral in origin, attempts to characterize it were inconclusive because of difficulties in obtaining sufficient amounts of purified virions to permit its characterization. Recent improvements of purification procedures have allowed the physic chemical characterization of this virus. Purified IHHNV is a non-enveloped icosahedral particle averaging 22 nm in diameter, exhibiting a mean buoyant density of 1·40 g/ml in CsCl. The genome is a single molecule of ssDNA with an estimated size of 4·1 kb by molecule length measurement in transmission electron microscopy. As determined by SDS-PAGE, the particle contains four polypeptides with s of 74K, 47K, 39K and 37·5K, respectively. From its characteristics, this virus could be a member of the family.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-11-2657
1990-11-01
2024-12-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/11/JV0710112657.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-11-2657&mimeType=html&fmt=ahah

References

  1. Bell T. A., Lightner D. V. 1984; 1HHN virus: infectivity and pathogenicity studies in Penaeus stylirostris and Penaeus vannamei. Aquaculture 38:185–194
    [Google Scholar]
  2. Berns K. I., Muzyczka N., Hauswirth W. W. 1986; Replication of parvoviruses. In Fundamental Virology pp. 545–562 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  3. Bijlholt M., van Bruggen E. F. J. 1986; A model for the architecture of the hemocyanin from the arthropod Squilla mantis (Crustacea, Stomatopoda). European Journal of Biochemistry 155:339–344
    [Google Scholar]
  4. Bonami J. R. 1977; Les maladies virales des crustacés et des mollusques. Oceanis 3:154–175
    [Google Scholar]
  5. Bonami J. R. 1980 Recherches sur les infections virales des crustacés marins: étude des maladies à étiologie simple et complexe chez les décapodes des côtes françaises These Doct. Etat Université des Sciences et Techniques du Languedoc, Montpellier:
    [Google Scholar]
  6. Chao Y. C., Young S. Y.III Kim K. S., Scott H. A. 1985; A newly isolated densonucleosis virus from Pseudoplusia includens (Lepidoptera: noctuidae). Journal of Invertebrate Pathology 46:70–82
    [Google Scholar]
  7. Delain E., Brack C. 1974; Visualisation des molécules d’acides nucléiques. II. - Microversion de la technique de diffusion. Journal de Microscopie 21:217–224
    [Google Scholar]
  8. Devauchelle G. 1977; Ultrastructural characterization of an iridovirus from the marine worm Nereis diversicolor (O. F. Müller). Architecture of the virion and virus morphogenesis. Virology 81:237–246
    [Google Scholar]
  9. Foster C. A., Farley E. A., Johnson P. T. 1981; Virus like particles in cardiac cells of the brown shrimp Penaeus aztecus Ives. Journal of Submicroscopic Cytology 13:723–726
    [Google Scholar]
  10. Johnson P. T. 1978; Viral diseases of the blue crab Callinectes sapidus. Marine Fisheries Review 40:13–15
    [Google Scholar]
  11. Kuris A. M., Poinar G. O., Hess R., Morris T. J. 1979; Virus particles in an internal parasite, Portunion conformis (Crustacea: Isopoda: Entoniscidae), and its marine crab host, Hemigrapsus oregonensis. Journal of Invertebrate Pathology 34:26–31
    [Google Scholar]
  12. Layne E. 1957; Proteins and derivatives; spectrophotometric and turbimetric methods - protein estimation by ultraviolet absorption. Methods in Enzymology 3:451–454
    [Google Scholar]
  13. Lightner D. V. 1985; A review of the diseases of cultured penaeid shrimps and prawns with emphasis on recent discoveries and developments. In Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps pp. 79–103 Taki Y., Primavera J. H., Llobrera J. A. Edited by Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center;
    [Google Scholar]
  14. Lightner D. V. 1988; Diseases of penaeid shrimp. In Disease Diagnosis and Control in North American Marine Aquaculture, 2nd edn. pp. 8–127 Sindermann C. S., Lightner D. V. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  15. Lightner D. V., Redman R. M. 1985; A parvo-like virus disease of penaeid shrimp. Journal of Invertebrate Pathology 45:47–53
    [Google Scholar]
  16. Lightner D. V., Redman R. M., Bell T. A. 1983a; Infectious hypodermal and hematopoietic necrosis, a newly recognized virus disease of penaeid shrimp. Journal of Invertebrate Pathology 42:62–70
    [Google Scholar]
  17. Lightner D. V., Redman R. M., Bell T. A. 1983b; Detection of IHHN virus in Penaeus stylirostris and P. vannamei imported into Hawaii. Journal of the World Mariculture Society 14:212–225
    [Google Scholar]
  18. Lightner D. V., Bell T. A., Redman R. M. 1990a; A review of the known hosts, geographic range and current diagnostic procedures for virus diseases of cultured penaeid shrimp. In Advances in Tropical Mariculture: Pathology of Molluscs, Crustaceans and Finfish Weppe M. Edited by Tahiti: IFREMER;
    [Google Scholar]
  19. Lightner D. V., Redman R. M., Bell T. A., Thurman R. B. 1990b; Geographic dispersion of the viruses IHHN, MBV and HPV as a consequence of transfers and introductions of panaeid shrimp to new regions for aquaculture purposes. In A Symposium on Human Influences on Aquatic Ecosystems, Proceedings of the National Shellfisheries Association Rosenfield A., Mann R. Edited by
    [Google Scholar]
  20. Lu Y., Loh P. C., Brock J. A. 1989; Isolation, purification and characterization of infectious hypodermal and hematopoietic necrosis virus (IHHNV) from penaeid shrimp. Journal of Virological Methods 26:339–344
    [Google Scholar]
  21. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Mari J. 1987 Recherches sur les maladies virales du crustacé décapode marin Carcinus mediterraneus, Czemiavski 1884 Thèse Doctorat Université des Sciences et Techniques du Languedoc, Montpellier:
    [Google Scholar]
  23. Mari J., Bonami J.-R. 1988a; W2 virus infection of the crustacean Carcinus metitterraneus: a reovirus disease. Journal of General Virology 69:561–571
    [Google Scholar]
  24. Mari J., Bonami J. R. 1988b; PC84, a parvo-like virus from the crab Carcinus mediterraneus: pathological aspects, ultrastructure of the agent, and first biochemical characterization. Journal of Invertebrate Pathology 51:145–156
    [Google Scholar]
  25. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  26. Meyers T. R. 1979; A reo-like virus isolated from juvenile American oysters (Crassostrea virginica). Journal of General Virology 43:203–212
    [Google Scholar]
  27. Rose J. A. 1974; Parvovirus production. In Comprehensive Virology 3 pp. 1–61 Fraenkel-Conrat H., Wagner R. R. Edited by New York & London: Plenum Press;
    [Google Scholar]
  28. Rueckert R. R. 1976; On the structure and morphogenesis of picornaviruses. In Comprehensive Virology 6 pp. 131–213 Fraenkel-Conrat H., Wagner R. R. Edited by New York & London: Plenum Press;
    [Google Scholar]
  29. Rueckert R. R. 1986; Picornaviruses and their replication. In Fundamental Virology pp. 357–390 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  30. Summers M. D. 1977; Characterization of shrimp baculovirus. Ecological Research Series 60077–130 Washington, D.C: U.S. Environmental Protection Agency;
    [Google Scholar]
  31. Tijssen P., Kurstak E. 1979; Studies on the structure of the two infectious types of densonucleosis virus. Intervirology 11:261–267
    [Google Scholar]
  32. van Bruggen E. F. J. 1986; Electron microscopy of hemocyanins 1942-1986. Micron and MicroscopicaActa 17:167–173
    [Google Scholar]
  33. Weber K. Ø., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  34. Winton J. R., Lannan C. N., Fryer J. L., Hedrick R. P., Meyers T. R., Plumb J. A., Yamamoto T. 1987; Morphological and biochemical properties of four members of a novel group of reoviruses isolated from aquatic animals. Journal of General Virology 68:353–364
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-71-11-2657
Loading
/content/journal/jgv/10.1099/0022-1317-71-11-2657
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error