1887

Abstract

Three synthetic peptides derived from the amino acid sequence of VP of canine parvovirus (CPV) which were recently shown to represent three distinct T cell epitopes for BALB/c mice could prime BALB/c mice for a CPV-specific proliferative T cell response upon immunization. Proliferative responses of peripheral blood mononuclear cells (PBMC) from CPV-immunized dogs upon stimulation with these and other peptides, covering the major part of the sequence of VP′, identified the presence of T cell epitopes for this species. Most of these epitopes were recognized by PBMC from only a minority of the dogs tested. With three newly generated canine Thyl T cell clones, which recognized CPV antigen in association with major histocompatibility complex class II molecules, two distinct T cell epitopes were identified within the unique sequence of VP.

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1990-10-01
2021-10-22
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References

  1. Abou-Zeid C., Filley E., Steele J., Rook G. A. W. 1987; A simple new method for using antigens separated by polyacrylamide gel electrophoresis to stimulate lymphocytes in vitroafter converting bands cut from Western blots into antigen bearing particles. Journal of Immunological Methods 98:5–10
    [Google Scholar]
  2. Appel M., Parrish C. R. 1987; Canine parvovirus-type II. In Virus Infections of Carnivores, pp 69–92 Appel M. J. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  3. Appel M. J. G., Scott F. W., Carmichael L. E. 1979; Isolation and immunisation studies of canine parvo-like virus from dogs with haemorrhagic enteritis. Veterinary Record 105:156–159
    [Google Scholar]
  4. Arnon R. 1987 Synthetic Vaccines Boca Raton: CRC Press;
    [Google Scholar]
  5. Berzofsky J. A., Cornette J., Margalit H., Cease K., Delisi C. 1986; Molecular features of class II MHC restricted T-cell recognition of protein and peptide antigens: the importance of amphipathic structures. Current Topics in Microbiology and Immunology 130:13–24
    [Google Scholar]
  6. Binn L. N., Marchwicki R. H., Stephenson E. H. 1980; Establishment of a canine cell line: derivation, characterization and viral spectrum. American Journal of Veterinary Research 41:855–860
    [Google Scholar]
  7. Brett S. J., Cease K. B., Ouyang C. S., Berzofsky J. A. 1989; Fine specificity of T cell recognition of the same peptide in association with different I-A molecules. Journal of Immunology 143:771–779
    [Google Scholar]
  8. Burtonboy G., Coignoul F., Delferriere N., Pastoret P. P. 1979; Canine hemorrhagic enteritis: detection of viral particles by electron microscopy. Archives of Virology 61:1–11
    [Google Scholar]
  9. Carlson J., Rushlov K., Maxwell I., Maxwell F., Winston S., Hahn W. 1985; Cloning and sequence of DNA encoding structural proteins of the autonomous parvovirus feline panleukopenia virus. Journal of Virology 55:574–582
    [Google Scholar]
  10. Carmichael L. E., Joubert J. C., Pollock R. V. H. 1981; A modified live canine parvovirus strain with novel plaque characteristics. I. Viral attenuation and dog response. Cornell Veterinarian 71:408–427
    [Google Scholar]
  11. Doveren R. F. C., Buurman W. A., Schutte B., Groenewegen G., Van Der Linden C.J. 1985; Class II antigens on canine T lymphocytes. Tissue Antigens 25:255–265
    [Google Scholar]
  12. Erickson P. W., Merrifield R. B. 1976; Solid phase peptide synthesis. In The Proteins pp 255–527 Neurath H., Hill R. L. Edited by New York: Academic Press;
    [Google Scholar]
  13. Gagnon A. N., Povey R. C. 1979; A possible parvovirus associated with an epidemic gastroenteritis of dogs in Canada. Veterinary Record 104:263–264
    [Google Scholar]
  14. Geysen H. M., Meloen R. H., Barteling S. 1984; Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proceedings of the National Academy of Sciences U.S.A.: 813998
    [Google Scholar]
  15. Mckenzie J. L., Fabre J. W. 1981; Studies with a monoclonal antibody on the distribution of Thy-1 in the lymphoid tissue of the dog. Transplantation 31:275–282
    [Google Scholar]
  16. Margalit H., Spouge J. L., Cornette J. L., Cease K. B., Delisi C., Berzofsky J. A. 1987; Prediction of immunodominant helper T cell antigenic sites from the primary sequence. Journal of Immunology 138:2213
    [Google Scholar]
  17. Meunier P. C., Cooper B. J., Appel M. J. G., Lanieu M. E., Slauson D. O. 1985; Pathogenesis of canine parvovirus enteritis: sequential virus distribution and passive immunisation studies. Veterinary Pathology 22:617–624
    [Google Scholar]
  18. Osterhaus A. D. M. E., Van Steenis G., De Kreek P. 1980; Isolation of a virus closely related to feline panleukopenia virus from dogs with diarrhea. Zentralblatt für Veterinärmedizin B27:11–21
    [Google Scholar]
  19. Panina-Bordignon P., Tan A., Termijtelen A., Demotz S., Corradin G., Lanzavecchia A. 1989; Universally immunogenic T cell epitopes: promiscuous binding to human MHC class II and promiscuous recognition by T cells. European Journal of Immunology 19:2237–2242
    [Google Scholar]
  20. Paradiso P. R., Rhode S. L.III Singer I. I. 1982; Canine parvovirus: a biochemical and ultrastructural characterization. Journal of General Virology 62:113–125
    [Google Scholar]
  21. Parrish C. R., Carmichael L. E. 1983; Antigenic structure and variation of canine parvovirus type-2, feline panleukopenia virus and mink enteritis virus. Virology 129:401–414
    [Google Scholar]
  22. Parrish C. R., O’Connell P. H., Evermann J. F., Carmichael L. E. 1985; Natural variation of canine parvovirus. Science 230:1046
    [Google Scholar]
  23. Parrish C. R., Aquadro C. F., Carmichael L. E. 1988; Canine host range and a specific epitope map along with variant sequences in the capsid protein gene of canine parvovirus and related feline, mink, and raccoon parvovirus. Virology 166:293–307
    [Google Scholar]
  24. Pollock R. V. H., Carmichael L. E. 1982; Maternally derived immunity to canine parvovirus infection: transfer, decline and interference with vaccination. Journal of the American Veterinary Medical Association 180:37
    [Google Scholar]
  25. Reed A. P., Jones E. V., Miller T. J. 1988; Nucleotide sequence and genome organization of canine parvovirus. Journal of Virology 62:266–276
    [Google Scholar]
  26. Rimmelzwaan G. F., Groen J., Juntti N., Teppema J. S., Uytdehaag F. G. C. M., Osterhaus A. D. M. E. 1987; Purification of infectious canine parvovirus from cell culture by affinity chromatography with monoclonal antibodies. Journal of Virological Methods 15:313–322
    [Google Scholar]
  27. Rimmelzwaan G. F., Vanderheijden R. W. J., Tijhaar E., Poelen M. C. M., Carlson J., Osterhaus A. D. M. E., Uytdehaag F. G. C. M. 1990; Establishment and characterization of canine parvovirus-specific murine CD4+T cell clones and their use for the delineation of T cell epitopes. Journal of General Virology 71:1095–1102
    [Google Scholar]
  28. Rhode S. L. 1985; Nucleotide sequence of the coat protein gene of canine parvovirus. Journal of Virology 54:630–633
    [Google Scholar]
  29. Surleraux M., Bodeus M., Burtonboy G. 1987; Study of canine parvovirus polypeptides by immunoblot analysis. Archives of Virology 95:271–281
    [Google Scholar]
  30. Swain S. L. 1983; T cell subsets and the recognition of MHC class. Immunological Reviews 74:129
    [Google Scholar]
  31. Van Der Zee R., Vaneden W., Meloen R. H., Noordzij A., Van Embden J. D. A. 1989; Efficient mapping and characterization of a T cell epitope by the simultaneous synthesis of multiple peptides. European Journal of Immunology 19:43–47
    [Google Scholar]
  32. Vanwezel A. L., Van Steenis G., Hannik C. A., Cohen H. 1978; New approaches to the production of concentrated and purified inactivated polio and rabies tissue culture vaccines. Developments in Biological Standardization 41:159–168
    [Google Scholar]
  33. Ziola B., Smith R. H., Qualtiere L. F. 1987; In vitroproliferation of lymphocytes from cyclophosphamide pretreated mice immunized with antigen mixed with dimethyl dioctadecyl ammonium bromide. Journal of Immunological Methods 97:159–164
    [Google Scholar]
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