1887

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Volume 71, Issue 1

Reciprocal Enhancement of Gene Expression and Viral Replication Between Human Cytomegalovirus and Human immunodeficiency Virus Type 1 Free

Abstract

Biological interactions between human cytomegalovirus (HCMV) and the human immunodeficiency virus type 1 (HIV-1) were analysed in transfection and infection experiments, carried out in a human osteogenic sarcoma cell line (HOS) and in the same cell line chronically infected with HCMV (E155). When HOS and El55 cells were transfected with recombinant plasmids containing the HIV long terminal repeat (LTR) linked to the bacterial chloramphenicol acetyl- transferase (CAT) gene, LTR-directed CAT expression was 20 times higher in El55 cells than in HOS cells. HOS cells co-infected with HCMV and HIV-1 showed enhanced production of the HIV-1 p24 antigen. In reciprocal experiments, an increase in HCMV immediate early gene expression was observed when HCMV- infected HOS cells and El55 cells were either transfected with a recombinant plasmid containing the HIV transactivator gene (pTAT), or when infected with HIV-1. DNA hybridization analysis of E155 and HCMV-infected HOS cells revealed higher levels of HCMV DNA in cells transfected with pTAT than in cells transfected with other non-specific recombinant plasmids. El55 cells transfected with pTAT also produced higher titres of infectious HCMV than control cultures of El55 cells transfected with other recombinant plasmids, including pMTAT carrying a mutant gene. The functional reciprocity between HCMV and HIV is discussed with respect to its possible implications for the clinical development of AIDS.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology 1990
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1990-01-01
2024-04-26
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Reciprocal Enhancement of Gene Expression and Viral Replication Between Human Cytomegalovirus and Human immunodeficiency Virus Type 1
J. Gen. Virol. 71, 97 (1990); https://doi.org/10.1099/0022-1317-71-1-97
/content/journal/jgv/10.1099/0022-1317-71-1-97
/content/journal/jgv/10.1099/0022-1317-71-1-97
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