1887

Abstract

Biological interactions between human cytomegalovirus (HCMV) and the human immunodeficiency virus type 1 (HIV-1) were analysed in transfection and infection experiments, carried out in a human osteogenic sarcoma cell line (HOS) and in the same cell line chronically infected with HCMV (E155). When HOS and El55 cells were transfected with recombinant plasmids containing the HIV long terminal repeat (LTR) linked to the bacterial chloramphenicol acetyl- transferase (CAT) gene, LTR-directed CAT expression was 20 times higher in El55 cells than in HOS cells. HOS cells co-infected with HCMV and HIV-1 showed enhanced production of the HIV-1 p24 antigen. In reciprocal experiments, an increase in HCMV immediate early gene expression was observed when HCMV- infected HOS cells and El55 cells were either transfected with a recombinant plasmid containing the HIV transactivator gene (pTAT), or when infected with HIV-1. DNA hybridization analysis of E155 and HCMV-infected HOS cells revealed higher levels of HCMV DNA in cells transfected with pTAT than in cells transfected with other non-specific recombinant plasmids. El55 cells transfected with pTAT also produced higher titres of infectious HCMV than control cultures of El55 cells transfected with other recombinant plasmids, including pMTAT carrying a mutant gene. The functional reciprocity between HCMV and HIV is discussed with respect to its possible implications for the clinical development of AIDS.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-1-97
1990-01-01
2022-10-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/1/JV0710010097.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-1-97&mimeType=html&fmt=ahah

References

  1. Adachi A., Koening S., Gendelman H. E., Daugherty D., Gattoni-Celu S., Fauci A. A., Martin M. A. 1987; Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. Journal of Virology 61:209–213
    [Google Scholar]
  2. Casareale D., Fiala M., Chang C. M., Cone L. A., Mocarski E. S. 1989; Cytomegalovirus enhances lysis of HIV-infected T lymphoblasts. International Journal of Cancer 44:124–130
    [Google Scholar]
  3. Cheng-Mayer C., Rutka J. T., Rosenblum M. L., Mchugh T., Stites D. P., Levy J. A. 1987; Human immunodeficiency virus can productively infect cultured human glial cells. Proceedings of the National Academy of Sciences U.S.A.: 843526–3530
    [Google Scholar]
  4. Colimon R., Mazeron M. C., Roseto A., Perol Y. 1984; Monoclonal antibodies (Me Ab): a new approach to the diagnosis of CMV infections. In CMV: Pathogenesis and Prevention of Human Infection pp 390–391 Plotkin S. A. Edited by New York: Alan R. Liss;
    [Google Scholar]
  5. Curran J. W., Morgan W. M., Hardy A. M., Jaffe H. W., Darrow W. W., Dowdle W. R. 1985; The epidemiology of AIDS: current status and future prospects. Science 229:1352–1357
    [Google Scholar]
  6. Davis M. G., Kenney S. C., Kamine J., Pagano J. S., Huang E.-S. 1987; Immediate-early gene region of human cytomegalovirus trans-activates the promoter of human immunodeficiency virus. Proceedings of the National Academy of Sciences U.S.A.: 848642–8646
    [Google Scholar]
  7. Drew W. L., Sweet E. S., Miner R. C., Mocarski E. S. 1984; Multiple infections by cytomegalovirus in patients with acquired immunodeficiency syndrome: documentation by Southern blot hybridization. Journal of Infectious Diseases 150:952–953
    [Google Scholar]
  8. Drew W, Mills J., Levy J., Dylewski J., Casavant C., Ammann A. J., Brodie H., Merigan T. 1985; Cytomegalovirus infections and abnormal T-lymphocyte subset ratios in homosexual men. Armais of Internal Medicine 103:61–63
    [Google Scholar]
  9. Elfassi E., Michelson S., Bachelevis F., Arenzana-Seisdeodos F. 1987; Transactivation of human immunodeficiency virus long terminal repeat during the early phases of human cytomegalovirus infection. Annales de l’Institut Pasteur/Virology 138:461–470
    [Google Scholar]
  10. Fiala M., Cone L. A., Chang C. M., Mocarski E. S. 1986; Cytomegalovirus viremia increases with progressive immune deficiency in patients infected with HTLV-III. AIDS Research 2:175–181
    [Google Scholar]
  11. Furukawa T., Herold R., Zolnick P., Plotkin S. A. 1981; Application of a human osteogenic sarcoma cell culture for detection of human cytomegalovirus antibody by immunofluorescence tests. Journal of Clinical Microbiology 13:206–208
    [Google Scholar]
  12. Gallo R. C., Salahuddin S. Z., Popovic M., Shearer G. M., Kaplan M., Haynes B. F., Palker T. J., Redfield R., Oleske J., Safai B., White G., Foster P., Markham P. D. 1984; Frequent detection and isolation of cytopathic retroviruses (HTLV- III) from patients with AIDS and risk for AIDS. Science 224:500–503
    [Google Scholar]
  13. Gendelman H. E., Phelps W., Feigenbaum L., Ostrove J. M., Adachi A., Howley P. M., Khoury G., Ginsberg H. S., Martin M. A. 1986; Trans-activation of the human immunodeficiency virus long terminal repeat sequence by DNA viruses. Proceedings of the National Academy of Sciences U.S.A.: 839759–9763
    [Google Scholar]
  14. Gimble J. M., Duh E., Ostrove J. M., Gendelman H. E., Max E. E., Rabson A. B. 1988; Activation of the human immunodeficiency virus long terminal repeat by herpes simplex virus type 1 is associated with induction of a nuclear factor that binds to the NF-kB/core enhancer sequence. Journal of Virology 62:4104–4112
    [Google Scholar]
  15. Gorman C. M., Moffat L. F., Howard B. H. 1982; Recombinant genomes which express chloramphenicol acetyltrans- ferase in mammalian cells. Molecular and Cellular Biology 2:1044–1051
    [Google Scholar]
  16. Graham F. L., Van Der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  17. Harouse J. M., Kunsch C., Hartle H. T., Laughlin M. A., Hoxie J. A., Wigdahl B., Gonzalez-Scarano F. 1989; CD4- independent infection of human neuronal cells by human immunodeficiency virus type 1. Journal of Virology 63:2527–2533
    [Google Scholar]
  18. Heieren M. H., Van Der Woude F. J., Balfour H. H. Jr 1988; Cytomegalovirus replicates efficiently in human kidney mesangial cells. Proceedings of the National Academy of Sciences U.S.A.: 851642–1646
    [Google Scholar]
  19. Hermiston T. W., Malone C. L., Witte P. R., Stinski M. F. 1987; Identification and characterization of the human cytomegalovirus immediate-early region 2 gene that stimulates gene expression from an inducible promoter. Journal of Virology 61:3214–3221
    [Google Scholar]
  20. Hirsch M. S., Felsenstein D. 1984; Cytomegalovirus-induced immunosuppression. Annals of the New York Academy of Sciences 437:8–15
    [Google Scholar]
  21. Ho W.-Z., Plotkin S. A. 1987; Rapid detection of cytomegalovirus infection by a tissue culture-centrifugation-monoclonal anti- body-biotin/avidin immunofluorescence technique. Molecular and Cellular Probes 1:83–93
    [Google Scholar]
  22. Horvat R. T., Wood C., Balachandran N. 1989; Transactivation of human immunodeficiency virus promoter by human herpes virus 6. Journal of Virology 63:970–973
    [Google Scholar]
  23. Hoxie J. A., Alpers J. D., Rackhowsky D., Huebner K., Haggarty A. J., Cedarbaum A. J., Reed J. C. 1986; Alterations in T4 (CD4) protein and mRNA synthesis in cells infected with HIV. Science 234:1123–1127
    [Google Scholar]
  24. Knight D. M., Flomerfelt F. A., Ghrayeb J. 1987; Expression of the art/trs protein of HIV and study of its role in viral envelope synthesis. Science 236:837–840
    [Google Scholar]
  25. Levy J. A., Hoffman A. D., Kramer S. M., Lanais J. A., Shimbukuro J. M., Oshiro L. S. 1984; Isolation of lymphocyto- pathic retroviruses from San Francisco patients with AIDS. Science 225:840–842
    [Google Scholar]
  26. Macher A. M., Reichert C. M., Straus S. E., Longo D. L., Parrillo J., Lane H. C., Fauci A. S., Rook A. H., Man-ISCHEWITZ J. F., Quinnan G. V. Jr 1983; Death in the AIDS patient. Role of cytomegalovirus. New England Journal of Medicine 309:14–54
    [Google Scholar]
  27. Mintz L., Drew W. L., Miner R. C., Braff E. H. 1983; Cytomegalovirus infections in homosexual men, an epidemiological study. Annals of Internal Medicine 99:326–329
    [Google Scholar]
  28. Mosca J. D., Bednarik D. P., Raj N.B.K., Rosen C. A., Sodroski J. G., Haseltine W. A., Pitha P. M. 1987; Herpes simplex virus type-1 can reactivate transcription of latent human immunodeficiency virus. Nature; London: 32567–70
    [Google Scholar]
  29. Nabel G., Baltimore D. 1987; An inducible transcription factor activates expression of human immunodeficiency virus in T cells. Nature; London: 326711–713
    [Google Scholar]
  30. Nelson J. A., Reynolds-Kohler C., Oldstone M. B. A., Wiley C. A. 1988; HIV and HCMV coinfect brain cells in patients with AIDS. Virology 165:286–290
    [Google Scholar]
  31. Osborn M., Debus E., Weber K. 1984; Monoclonal antibodies specific for vimentin. European Journal of Cell Biology 34:137–143
    [Google Scholar]
  32. Peterlin B. M., Luciw P. A., Barr P. J., Walker M. D. 1986; Elevated levels of mRNA can account for the trans-activation of human immunodeficiency virus. Proceedings of the National Academy of Sciences U.S.A.: 839734–9738
    [Google Scholar]
  33. Pizzorno M. C., O’Hare P., Sha L., LaFemina R. L., Hayward G. S. 1988; Trans-activation and autoregulation of gene expression by the immediate-early region 2 gene products of human cytomegalovirus. Journal of Virology 62:1167–1179
    [Google Scholar]
  34. Polk B. F., Fox R., Brookmeyer R., Kanchanaraksa S., Kaslow R., Visscher B., Rinaldo C., Phair J. 1987; Predictors of the acquired immunodeficiency syndrome developing in a cohort of seropositive homosexual men. New England Journal of Medicine 316:61–66
    [Google Scholar]
  35. Rando R. F., Groff D. E., Chirikjian J. G., Lancaster W. D. 1986; Isolation and characterization of a novel human papillomavirus type 6 DNA from an invasive vulvar carcinoma. Journal of Virology 57:353–356
    [Google Scholar]
  36. Rando R. F., Pellett P. E., Luciw P. A., Bohan C. A., Srinivasan A. 1987; Transactivation of human immunodeficiency virus by herpes viruses. Oncogene 1:13–18
    [Google Scholar]
  37. Rhim J. S., Cho H. Y., Huebner R. J. 1975; Non-producer cells induced by murine sarcoma virus. International Journal of Cancer 15:23–24
    [Google Scholar]
  38. Rice G.P.A., Schrier R. D., Oldstone M.B.A. 1984; Cytomegalovirus infects human lymphocytes and monocytes: virus expression is restricted to immediate-early gene products. Proceed-ings of the National Academy of Sciences U.S.A.: 816134–6138
    [Google Scholar]
  39. Rubin R. H., Cosimi A. B., Tolkoff-Rubin N. E., Russell P. S., Hirsch M. S. 1977; Infectious disease syndromes attributable to cytomegalovirus and their significance among renal transplant recipients. Transplantation 24:458–464
    [Google Scholar]
  40. Sanchez-Pescador R., Power M. D., Barr P. J., Steimer K. S., Stempien M. M., Brown-Shimer S. L., Gee W. W., Renard A., Randolph A., Levy J. A., Dina D., Luciw P. A. 1985; Nucleotide sequence and expression of an AIDS-associated retrovirus (ARV-2). Science 227:484–492
    [Google Scholar]
  41. Singh N., Dummer J. S., Kusne S., Breining M. K., Armstrong J. A., Makowka L., Starzi T., Ho M. 1988; Infection with cytomegalovirus and other herpesviruses in 121 liver transplant recipients: transmission by donated organ and the effect of OKT3 antibodies. Journal of Infectious Diseases 158:124–131
    [Google Scholar]
  42. Skolnik P. R., Kosloff B. R., Hirsch M. S. 1988; Bidirectional interactions between human immunodeficiency virus type 1 and cytomegalovirus. Journal of Infectious Diseases 157:508–514
    [Google Scholar]
  43. Spector S. A., Hirata K. K., Neuman T. R. 1984; Identification of multiple cytomegalovirus strains in homosexual men with acquired immunodeficiency syndrome. Journal of Infectious Diseases 150:953–956
    [Google Scholar]
  44. Srinivasan A., Anand R., York D., Ranganathan P., Feorino P., Schochetman G., Curran J., Kalyanaraman V. S., Luciw P. A., Sanchez-Pescador R. 1987; Molecular characterization of human immunodeficiency virus from Zaire : nucleotide sequence analysis identifies conserved and variable domains in the envelope gene. Gene 52:71–82
    [Google Scholar]
  45. Stenberg R. M., Thomsen D. R., Stinski M. F. 1984; Structural analysis of the major immediate early gene of human cytomegalovirus. Journal of Virology 49:190–199
    [Google Scholar]
  46. Stinski M. F., Thomsen D. R., Stenberg R. M., Goldstein L. C. 1983; Organization and expression of the immediate-early genes of human cytomegalovirus. Journal of Virology 46:1–14
    [Google Scholar]
  47. Tateno M., Gonzalez-Scarano F., Levy J. A. 1989; Human immunodeficiency virus can infect CF4-negative human fibroblas- toid cells. Proceedings of the National Academy of Sciences U.S.A.: 864287–4290
    [Google Scholar]
  48. Wentworth B. B., French L. 1970; Plaque assay of cytomegalovirus strains of human origin. Proceedings of the Society for Experimental Biology and Medicine 135:253–258
    [Google Scholar]
  49. Zagury D., Bernard J., Leonard R., Cheynier R., Feldman M., Sarin P. S., Gallo R. C. 1986; Long-term cultures of HTLV- Ill-infected T cells: a model of cytopathology of T-cell depletion in AIDS. Science 231:850–853
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-1-97
Loading
/content/journal/jgv/10.1099/0022-1317-71-1-97
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error