1887

Abstract

Human papillomavirus (HPV) types 6 and 11 are usually found in benign genital lesions and laryngeal papillomas. However, the occasional occurrence of their DNAs in carcinomas of the genital tract and larynx suggests that they have some tumorigenic activity. In this paper, we have examined the cotransforming and transactivation activities of the E7 genes from these virus types and show that they cooperate with to transform primary cells, but at a greatly reduced level compared to HPV-16 E7.Although the efficiencies of transformation by HPV-6 and HPV-11 are low, it is striking that the cells that are transformed are highly tumorigenic in immunocompetent animals. Transactivation studies using the adenovirus E2 promoter demonstrated that both HPV-11 E7 and HPV-16 E7 could stimulate transcription to a similar degree. These results separate the transactivation and co-transforming activities of HPV E7 genes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-1-165
1990-01-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/1/JV0710010165.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-1-165&mimeType=html&fmt=ahah

References

  1. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  2. Chirgwin J., Przybyla A. E., Macdonald R. J., Rutter W. J. 1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299
    [Google Scholar]
  3. Cole S. T., Danos O. 1987; Nucleotide sequence and comparative analysis of the human papillomavirus 18 genome. Phylogeny of papillomaviruses and repeated structure of the E6 and E7 gene products. Journal of Molecular Biology 193:599–608
    [Google Scholar]
  4. Crook T., Storey A., Almond N., Osborn K., Crawford L. 1988; Human papillomavirus type 16 cooperates with activated rasand fos oncogenes in the hormone-dependent transformation of primary mouse cells. Proceedings of the National Academy of Sciences, U.S.A 85:8820–8824
    [Google Scholar]
  5. Crook T., Morgenstern J., Crawford L., Banks L. 1989; Continued expression of HPV-16 E7 protein is required for maintenance of the transformed phenotype of cells cotransformed by HPV-16 plus EJ-ras . EMBO Journal 8:513–519
    [Google Scholar]
  6. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  7. Feldman L. T., Imperiale M. J., Nevins J. R. 1982; Activation of early adenovirus transcription by the herpes immediate early gene: evidence for a common cellular control factor. Proceedings of the National Academy of Sciences, U.S.A 79:4952–4956
    [Google Scholar]
  8. Ferguson B., Kripple B., Andrisani O., Jones N., Westphal H., Rosenberg M. 1985; Ela 13S and 12S mRNA products made in Escherichia coli both function as nucleus localized transcription activators but do not directly bind DNA. Molecular and Cellular Biology 5:2653–2661
    [Google Scholar]
  9. Glenn G. M., Ricciardi R. P. 1985; Adenovirus 5 early region 1A host range mutants hr 3, hr 4 and hr 5 contain point mutations which generate single amino acid substitutions. Journal of Virology 56:66–74
    [Google Scholar]
  10. Hall C. V., Jacob P. E., Ringold G. M., Lee F. 1983; Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells. Journal of Molecular and Applied Genetics 2:101–109
    [Google Scholar]
  11. Kasher M. S., Roman A. 1988; Characterization of human papillomavirus type 6b DNA isolated from an invasive squamous carcinoma of the vulva. Virology 165:225–233
    [Google Scholar]
  12. Kimelman D., Millar J. S., Porter D., Roberts B. E. 1985; Ela regions of the human adenoviruses and of the highly oncogenic simian adenovirus 7 are closely related. Journal of Virology 53:399–409
    [Google Scholar]
  13. Land H., Chen A. C., Morgenstern J. P., Parada L. F., Weinberg R. A. 1986; Behaviour of myc and ras oncogenes in transformation of rat embryo fibroblasts. Molecular and Cellular Biology 6:1917–1925
    [Google Scholar]
  14. Lillie J. W., Green M., Green M. R. 1986; An adenovirus Ela protein region required for transformation and transcriptional repression. Cell 46:1043–1051
    [Google Scholar]
  15. Lillie J. W., Loewenstein P. M., Green M. R., Green M. 1987; Functional domains of adenovirus type 5 Ela proteins. Cell 50:1091–1100
    [Google Scholar]
  16. Miller J. H. 1972 Experiments in Molecular Genetics New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Moran E., Zerler B., Harrison T. M., Matthews M. B. 1986; Identification of separate domains in the adenovirus Ela gene for immortalization activity and the activation of virus early genes. Molecular and Cellular Biology 6:3470–3480
    [Google Scholar]
  18. Murthy S. C. S., Bhat G. P., Thimmappaya B. 1985; Adenovirus Ela early promoter: transcriptional control elements and induction by the viral pre-early Ela gene which appears to be sequence independent. Proceedings of the National Academy of Sciences, U.S.A 82:2230–2234
    [Google Scholar]
  19. Phelps W. C., Yee C. L., Munger K., Howley P. M. 1988; The human papillomavirus type 16 E7 gene encodes transactivation and transformation functions similar to those of adenovirus Ela. Cell 53:539–547
    [Google Scholar]
  20. Rando R. F., Groff D. E., Chirikjian J. G., Lancaster W. D. 1986; Isolation and characterization of a novel human papillomavirus type 6 DNA from an invasive vulval carcinoma. Journal of Virology 57:353–356
    [Google Scholar]
  21. Reed K. C., Mann D. A. 1985; Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Research 13:7207–7221
    [Google Scholar]
  22. Schlegel R., Phelps W. C., Zhang Y-L., Barbosa M. 1988; Quantitative keratinocyte assay detects two biological activities of human papillomavirus DNA and identifies viral types associated with cervical carcinoma. EMBO Journal 7:3181–3187
    [Google Scholar]
  23. Schneider J. F., Fisher F., Goding C. R., Jones N. C. 1987; Mutational analysis of the adenovirus Ela gene: the role of transcriptional regulation in transformation. EMBO Journal 6:2053–2060
    [Google Scholar]
  24. Shih C., Weinberg R. A. 1982; Isolation of a transforming sequence from a human bladder carcinoma cell line. Cell 29:161–169
    [Google Scholar]
  25. Smotkin D., Wettstein F. O. 1986; Transcription of human papillomavirus type 16 early genes in a cervical cancer and cancer- derived cell line and identification of the E7 protein. Proceedings of the National Academy of Sciences, U.S.A 83:4680–4684
    [Google Scholar]
  26. Smotkin D., Prokoph H., Wettstein F. O. 1989; Oncogenic and nononcogenic human genital papillomaviruses generate the E7 mRNA by different mechanisms. Journal of Virology 63:1441–1447
    [Google Scholar]
  27. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  28. Southern P. J., Berg P. 1982; Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. Journal of Molecular and Applied Genetics 1:327–341
    [Google Scholar]
  29. Spalholz B. A., Yang Y-C., Howley P. M. 1985; Transactivation of a bovine papillomavirus transcriptional regulatory element by the E2 gene product. Cell 42:183–191
    [Google Scholar]
  30. Storey A., Pim D., Murray A., Osborn K., Banks L., Crawford L. 1988; Comparison of the in vitro transforming activities of human papillomavirus types. EMBO Journal 7:1815–1820
    [Google Scholar]
  31. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U.S.A 77:5201–5205
    [Google Scholar]
  32. Van Ormondt H., Maat J., Dijkema R. 1980; Comparison of nucleotide sequences of the early Ela regions for subgroups A, B and C of human adenoviruses. Gene 12:63–76
    [Google Scholar]
  33. Wigler M., Pellicer A., Silverstein S., Axel R., Urlaub G., Chasin L. 1979; DNA-mediated transfer of the adenine phosphoribosyl transferase locus into mammalian cells. Proceedings of the National Academy of Sciences, U.S.A 76:1373–1376
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-1-165
Loading
/content/journal/jgv/10.1099/0022-1317-71-1-165
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error