1887

Abstract

Summary

Genetic reassortment of Thogoto (THO) virus has been demonstrated in dually infected ticks. However previous results showed that oral superinfection is inhibited by interference. To ascertain the site of THO viral interference, ticks were infected parenterally or orally with a temperature-sensitive () mutant of THO virus. Infected ticks were then challenged with wild-type () THO virus via parenteral inoculation. Intra-stadial superinfection was carried out by parenteral inoculation of newly infected engorged ticks whereas inter-stadial superinfection involved inoculation of engorged ticks infected at the previous stage. In both instances viral interference was not observed, i.e. the challenge virus replicated and was delivered by bite to susceptible hosts. Therefore when the gut is bypassed, . ticks are apparently permissive to dual infection even when there is a delay in the presentation of the superinfecting virus. These results demonstrate that interference following superinfection does not occur in the salivary glands, but may occur in the gut and possibly in a secondary site of viral replication such as the synganglion.

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/content/journal/jgv/10.1099/0022-1317-70-9-2469
1989-09-01
2021-10-17
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References

  1. Booth T. F., Davies C. R., Jones L. D., Staunton D., Nuttall P. A. 1989; Anatomical basis of Thogoto virus infection in BHK cell culture and in the ixodid tick vector, Rhipicephalus appendiculatus . Journal of General Virology 70:1092–1104
    [Google Scholar]
  2. Davies C. R., Jones L. D., Nuttall P. A. 1986; Experimental studies on the transmission cycle of Thogoto virus, a candidate orthomyxovirus, in Rhipicephalus appendiculatus ticks. American Journal of Tropical Medicine and Hygiene 35:1256–1262
    [Google Scholar]
  3. Davies C. R., Jones L. D., Green B. M., Nuttall P. A. 1987; In vivo reassortment of Thogoto virus (a tick-borne influenza-like virus) following oral infection of Rhipicephalus appendiculatus ticks. Journal of General Virology 68:2331–2338
    [Google Scholar]
  4. Davies C. R., Jones L. D., Nuttall P. A. 1989; Viral interference in the tick, Rhipicephalus appendiculatus. I. Interference to oral superinfection by Thogoto virus. Journal of General Virology 70:2461–2468
    [Google Scholar]
  5. Jones L. D., Davies C. R., Green B. M., Nuttall P. A. 1987; Reassortment of Thogoto virus (a tick-borne influenza-like virus) in a vertebrate host. Journal of General Virology 68:1299–1306
    [Google Scholar]
  6. Jones L. D., Davies C. R., Steele G. M., Nuttall P. A. 1988; The rearing and maintenance of ixodid and argasid ticks in the laboratory. Journal of Animal Technology 39:99–106
    [Google Scholar]
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