1887

Abstract

Summary

Intact virions of robinia mosaic virus (RoMV) which were inactive in a rabbit reticulocyte translation system when suspended in 10 m-phosphate buffer pH 6.0, exhibited an efficient translation activity after treatment with 10 m-phosphate buffer pH 8.0. The estimated values of the translation products of the intact virions were 130K, 93K, 77K, 60K, 43K, 34K and 31K by SDS-PAGE. In comparison with the treated intact virions, the total viral genomic RNA produced only three polypeptides, of approx. 31K, 25K and 22K. Circular dichroism (CD) measurements of the virions showed that the α-helical structure of the viral coat protein subunits might undergo some conformational changes after treatment under mildly alkaline conditions. Fluorescence emission and quenching analysis indicated that the microenvironment of chromophores in the virions suspended in 10 m-phosphate buffer pH 6.0 might be different from that of the virions in 10 m-phosphate buffer pH 8.0. Both CD and fluorescence evidence suggested that as a result of conformational changes in the viral coat protein subunits, the fine structure of the virions might be altered after short exposure to the mildly alkaline buffer, although such alteration was undetectable by electron microscopy. The conformational changes of the viral coat protein subunits and consequently the alteration of the fine structure of the virions could facilitate the gene expression and uncoating of RoMV .

Keyword(s): RoMV , translation and uncoating
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-9-2271
1989-09-01
2019-11-21
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-9-2271
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error