1887

Abstract

SUMMARY

A 2330 nucleotide sequence spanning the 1B (NS2), IC (NS1) and N genes and intergenic regions of human respiratory syncytial virus strain 18537, representing antigenic subgroup B, was determined by sequencing cloned cDNAs of intracellular mRNAs. Comparison with the previously reported sequences for strain A2 of subgroup A showed that 1B, 1C and N were highly conserved at the nucleotide level (78, 78 and 86% identity, respectively) and at the amino acid level (92, 87 and 96% identity, respectively). The gene-start signals were exactly conserved between subgroups, and the gene-end signals contained only a single nucleotide substitution each in 1B and N. In most cases intergenic and non-coding gene sequences that were not part of presumed transcriptive signals were much less well conserved (generally 50 to 71%) than sequences that were part of translational open reading frames (82 to 86%). The nucleotide and deduced amino acid sequences of the N gene and protein of the Long strain of subgroup A were determined by sequencing cDNA clones of intracellular mRNA; the nucleotide sequence (representing all but the first 10 nucleotides of the gene) contained 15 differences from that of the A2 strain, but the deduced amino acid sequences were identical.

Keyword(s): evolution , human , nucleotide sequence and RSV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-6-1539
1989-06-01
2022-08-11
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/6/JV0700061539.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-6-1539&mimeType=html&fmt=ahah

References

  1. Åkerlind B., Norrby E. 1986; Occurrence of respiratory syncytial virus subtypes A and B strains in Sweden.. Journal of Medical Virology 19:241–247
    [Google Scholar]
  2. Anderson L. J., , Hierholzer J. C., , Tsos C., , Hendry R. M., , Ferme B. F., , Store Y., Mcintosh K. 1985; Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies.. Journal of Infectious Diseases 151:626–633
    [Google Scholar]
  3. Coates H. V., , Alling D. W., Chanock R. M. 1966; An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test.. American Journal of Epidemiology 83:299–313
    [Google Scholar]
  4. Coelingh K. L. V., , Winter C. C., Murphy B. R. 1988; Nucleotide and deduced amino acid sequence of hemagglutinin-neuraminidase genes of human type 3 parainfluenza viruses isolated from 1957 to 1983.. Virology 162:137–143
    [Google Scholar]
  5. Collins P. L., Wertz G. W. 1983; cDNA cloning and transcriptional mapping of nine polyadenylated RNAs encoded by the genome of human respiratory syncytial virus.. Proceedings of the National Academy of Sciences, U.S.A. 80:3208–3212
    [Google Scholar]
  6. Collins P. L., Wertz G. W. 1985; Nucleotide sequences of the 1B and 1C nonstructural protein mRNAs of human respiratory syncytial virus.. Virology 143:442–451
    [Google Scholar]
  7. Collins P. L., , Huang Y. T., Wertz G. W. 1984; Identification of a tenth mRNA of respiratory syncytial virus and assignment of polypeptides to the 10 viral genes.. Journal of Virology 49:572–578
    [Google Scholar]
  8. Collins P. L., , Anderson K., , Langer S. J., Wertz G. W. 1985; Correct sequence for the major nucleocapsid protein mRNA of respiratory syncytial virus.. Virology 146:69–77
    [Google Scholar]
  9. Collins P. L., , Dickens L. E., , Buckler-White A., , Olmsted R. A., , Spriggs M. K., , Camargo E., Coelingh K. V. W. 1986; Nucleotide sequences for the gene junctions of human respiratory syncytial virus reveal distinctive features of intergenic structure and gene order.. Proceedings of the National Academy of Sciences, U.S.A. 83:4594–4598
    [Google Scholar]
  10. Collins P. L., , Olmsted R. A., , Spriggs M. K., , Johnson P. R., buckler-White A. J. 1987; Gene overlap and sitespecific attenuation of transcription of the viral polymerase L gene of human respiratory syncytial virus.. Proceedings of the National Academy of Sciences, U.S.A. 84:5134–5138
    [Google Scholar]
  11. Dickens L. E., , Collins P. L., Wertz G. W. 1984; Transcriptional mapping of human respiratory syncytial virus.. Journal of Virology 52:364–369
    [Google Scholar]
  12. Gimenez H. B., , Hardman N., , Keir H. M., Cash P. 1986; Antigenic variation between human respiratory syncytial virus isolates.. Journal of General Virology 67:863–870
    [Google Scholar]
  13. Hendry R. M., , Talis A. L., , Godfrey E., , Anderson L. J., , Fernie B. F., Mcintosh K. 1986; Concurrent circulation of antigenically distinct strains of respiratory syncytial virus during community outbreaks.. Journal of Infectious Diseases 153:291–297
    [Google Scholar]
  14. Huang Y. T., , Collins P. L., Wertz G. W. 1985; Characterization of the 10 proteins of human respiratory syncytial virus: identification of a fourth envelope-associated protein.. Virus Research 2:157–173
    [Google Scholar]
  15. Johnson P. R., Collins P. L. 1988a; The fusion glycoproteins of human respiratory syncytial virus of subgroups A and B: sequence conservation provides a structural basis for antigenic relatedness.. Journal of General Virology 69:2623–2628
    [Google Scholar]
  16. Johnson P. R., Collins P. L. 1988b; The A and B subgroups of human respiratory syncytial virus: comparison of intergenic and gene-overlap sequences.. Journal of General Virology 69:2901–2906
    [Google Scholar]
  17. Johnson P. R., , Olmsted R. A., , Prince G. A., , Murphy B. R., , Alling D. W., , Walsh E. E., Collins P. L. 1987a; Antigenic relatedness between the glycoproteins of human respiratory syncytial virus subgroups A and B: evaluation of the contributions of the F and G glycoproteins to immunity.. Journal of Virology 61:3163–3166
    [Google Scholar]
  18. Johnson P. R., , Spriggs M. K., , Olmsted R. A., Collins P. L. 1987b; The G glycoproteins of human respiratory syncytial virus subgroups A and B : extensive sequence divergence between antigenically related proteins.. Proceedings of the National Academy of Sciences, U.S.A. 84:5625–5629
    [Google Scholar]
  19. Lopez J. A., , Villanueva N., , Melerò J. A., Portela A. 1988; Nucleotide sequence of the fusion and phosphoprotein genes of human respiratory syncytial (RS) virus Long strain: evidence of subtype genetic heterogeneity.. Virus Research 10:249–262
    [Google Scholar]
  20. Mcintosh K. M., Chanock R. M. 1985; Respiratory syncytial virus.. In Virology1285–1304 Fields B. N. New York: Raven Press;
    [Google Scholar]
  21. Morgan L. A., , Routledge E. G., , Willcocks M. M., , Samson A. c. R., , Scott R., Toms G. L. 1987; Strain variation of respiratory syncytial virus.. Journal of General Virology 68:2781–2788
    [Google Scholar]
  22. Mufson M. A., , Örvell C., , Rafnar B., Norrby E. 1985; Two distinct subtypes of human respiratory syncytial virus.. Journal of General Virology 66:2111–2124
    [Google Scholar]
  23. Mufson M. A., , Belshe R. B., , Örvell C., Norrby E. 1987; Subgroup characteristics of respiratory syncytial virus strains recovered from children with two consecutive infections.. Journal of Clinical Microbiology 25:1535–1539
    [Google Scholar]
  24. Örvell C., , Norrby E., Mufson M. A. 1987; Preparation and characterization of monoclonal antibodies directed against five structural components of human respiratory syncytial virus subgroup B.. Journal of General Virology 68:3125–3135
    [Google Scholar]
  25. Sakai Y., , Suzu S., , Shioda T., Shibuta H. 1987; Nucelotide sequence of the bovine parainfluenza 3 virus genome: its 3′ end and the genes of NP, P, C and M proteins.. Nuecleic Acids Research 15:2927–2944
    [Google Scholar]
  26. Stott E. J., , Taylor G., , Ball L. A., , Anderson K., , Young K. K., -Y., King A. M. Q., Wertz G. W. 1987; Immune and histopathological responses in animals vaccinated with recombinant vaccinia viruses that express individual genes of human respiratory syncytial virus.. Journal of Virology 61:3855–3861
    [Google Scholar]
  27. Suzu S., , Sakai Y., , Shioda T., Shibuta H. 1987; Nucleotide sequence of the bovine parainfluenza 3 virus genome: the genes of the F and HN glycoproteins.. Nucleic Acids Research 15:2945–2958
    [Google Scholar]
  28. Walsh E. E., , Brandriss M. W., Schlesinger J. J. 1987; Immunological differences between the envelope glycoproteins of two strains of human respiratory syncytial virus.. Journal of General Virology 68:2169–2176
    [Google Scholar]
  29. Zagursky R. J., , Baumeister K., , Lomax N., Berman M. L. 1985; Rapid and easy sequencing of large linear double stranded DNA and supercoiled plasmid DNA.. Gene Analytical Techniques 2:89–94
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-6-1539
Loading
/content/journal/jgv/10.1099/0022-1317-70-6-1539
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error