1887

Abstract

SUMMARY

The genome of a 22 nm icosahedral phage which infects some avian strains recovered from domestic ducks has been characterized as a ss circular DNA molecule of about 4850 bases. The replicative form of this genome was isolated from purified chlamydial organisms. A restriction endonuclease cleavage site map of the genome was constructed from dsDNA synthesized from ss phage DNA and RI fragments were then cloned into pUC9. The phage genome was detected only by Southern blot hybridization in which was productively infected with phage; no evidence was found for the integration of phage DNA into the chlamydial chromosome. Three viral polypeptides, of approximate values 75K, 30K and 16.5K were identified when phage was analysed by SDS–PAGE. This virus, which we have designated Chpl, is either an aberrant member of the or the first member of a new bacteriophage family.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-6-1321
1989-06-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/6/JV0700061321.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-6-1321&mimeType=html&fmt=ahah

References

  1. Andrews B. E., Major R., Palmer S. R. 1981; Ornithosis in poultry workers.. Lancet i:632–634
    [Google Scholar]
  2. Ashley C. R., Caul E. O. 1982; Potassium tartrate-glycerol as a density gradient substrate for separation of small, round viruses from human feces.. Journal of Clinical Microbiology 16:377–381
    [Google Scholar]
  3. Bacon E. J., Richmond S. J., Wood D. J., Stirling P., Bevan B. I., Chalmers W. S. K. 1986; Serological detection of phage infection in Chlamydia psittaci recovered from ducks.. Veterinary Record 119:618–620
    [Google Scholar]
  4. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA.. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  5. Burkardt H. J., Puhler A. 1984; Electron microscopy of plasmid DNA.. Methods in Microbiology 17:134–155
    [Google Scholar]
  6. Feinberg A. P., Vogelstein B. 1984; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.. Analytical Biochemistry 137:266–267
    [Google Scholar]
  7. Godson G. N. 1978; The other isometric phages.. In The Single-Stranded DNA Phages103–112 Denhardt D. T., Dressler D., Ray D. S. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Grunstein M., Hogness D. S. 1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.. Proceedings of the National Academy of Sciences, U.S.A. 72:3961–3965
    [Google Scholar]
  9. Kessler S. W. 1975; Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.. Journal of Immunology 115:1617–1624
    [Google Scholar]
  10. Kuo C. C., Caldwell H. D., Wang S. P., Grayston J. T. 1977; Antigens of Chlamydia trachomatis . In Nongonococcal Urethritis and Related Infections176–185 Hobson D., Holmes K. K. Washington, D. C.: American Society for Microbiology;
    [Google Scholar]
  11. Kushner S. R. 1978; An improved method for transformation of Escherichia coli with Col E1 derived plasmids.. In Genetic Engineering17–23 Boyer H. B., Nicosin S. Amsterdam: Elsevier;
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature, London 277:680–685
    [Google Scholar]
  13. Lusher M., Storey C. C., Richmond S. J. 1989; Plasmid diversity within the genus Chlamydia . Journal of General Microbiology 135:1145–1151
    [Google Scholar]
  14. Maniatis T., Frltsch E. F., Sambrook J. 1982; . Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Matthews R. E. F. 1982; Classification and nomenclature of viruses.. Intervirology 17:1–199
    [Google Scholar]
  16. Morgan H. R., Wiseman R. W. 1946; Growth of psittacosis virus in roller tube tissue culture; use in a vaccine.. Joumal of Infectious Diseases 79:131–133
    [Google Scholar]
  17. Richmond S. J., Stirling P., Ashley C. R. 1982; Virus infecting the reticulate bodies of an avian strain of Chlamydia psittaci . FEMS Microbiology Letters 14:31–36
    [Google Scholar]
  18. Rlchmond S. J., Bailey I. M. G., Bailey A. S., Mearns G. 1985; Primary isolation of Chlamydia trachomatis and Chlamydia psittaci in in vitro cell culture.. In Isolation and Identification of Microorganisms of Medical and Veterinary Importance297–312 Collins C. H., Grange J. M. New York: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-6-1321
Loading
/content/journal/jgv/10.1099/0022-1317-70-6-1321
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error