1887

Abstract

Summary

Infection by Thogoto (THO) virus, a tick-borne virus related to the orthomyxo-viruses, has been compared in vertebrate cell culture and in ticks using infectivity titrations, immunofluorescence, and immune electron microscopy with colloidal gold markers to detect cell surface and intracellular antigens. Morphogenesis of THO virus in cell culture was similar to that of influenza virus, with polymorphic virus particles budding at the plasma membrane. In the tick, THO viral infection caused no obvious pathology; virions or budding profiles were not observed in electron micrographs, although replication, trans-stadial persistence and transmission to a susceptible host occur. THO virus was not detected in the salivary glands of trans-stadially infected ticks until about 7 days after the commencement of feeding on a host. The synganglion (brain) appears to be the major organ involved in trans-stadial persistence of the virus; viral antigens were detected in the neural cortex (cell bodies) but not in nerve fibres and axons. The detection of THO viral antigen in basement membranes and connective tissue, but its absence from nerve fibres, suggests that dissemination occurs via the haemolymph rather than a neural route.

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1989-05-01
2022-01-23
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References

  1. Albanese M., Bruno-Smiraglia C., Di Di Cuonzo G., Lavagnino A., Srihongse S. 1972; Isolation of Thogoto virus from Rhipicephalus bursa ticks in Western Sicily. Ada virologica 16:267
    [Google Scholar]
  2. Arthur D. R. 1962 Ticks and Disease London, New York & Paris: Pergamon Press;
    [Google Scholar]
  3. Bächi T., Gerhard W., Lindemann I., Muhlethaler K. 1969; Morphogenesis of influenza A virus in Ehrlich ascites tumor cells as revealed by thin-sectioning and freeze etching. Journal of Virology 4:769–776
    [Google Scholar]
  4. Binnington K. C. 1983; Ultrastructural identification of neurohaemal sites in a tick: evidence that the dorsal complex may be a true endocrine gland. Tissue and Cell 15:317–327
    [Google Scholar]
  5. Burgdorfer W., Varma M. G. R. 1967; Trans-stadial and transovarial development of disease agents in arthropods. Animal Review of Entomology 2:347–376
    [Google Scholar]
  6. Chastel C., Guigen C., Quillien M. C., Le Lay-Rouges G., Beaucornu J. C. 1984; Visualisation du virus Soldado par la microscopie electronique dans les tissues de la tique vectrice Ornithodoros (Alectorobius) maritimus Vermeil et Marguet, 1967. Annals of Parasitology 59:1–6
    [Google Scholar]
  7. Chernesky M. A., McLean D. M. 1969; Localisation of Powassan virus in Dermacentor andersoni ticks by immunofluorescence. Canadian Journal of Microbiology 15:1399–1408
    [Google Scholar]
  8. Clerx J. P. M., Fuller F., Bishop D. H. L. 1983; Tick-borne viruses structurally similar to Orthomyxoviruses. Virology 127:205–219
    [Google Scholar]
  9. Compans R. W., Choppin P. W. 1975; Reproduction of myxoviruses. In Comprehensive Virology 4179–225 Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  10. Compans R. W., Dimmock N. J. 1969; An electron microscopic study of single-cycle infection of chick embryo fibroblasts by influenza virus. Virology 39:449–515
    [Google Scholar]
  11. Davies C. R., Jones L. D., Nuttall P. A. 1986; Experimental studies on the transmission cycle of Thogoto virus, a candidate orthomyxovirus, in Rhipicephalus appendiculatus ticks. American Journal of Tropical Medicine and Hygiene 35:1256–1262
    [Google Scholar]
  12. Enzmann P. J. 1987; A comparative view of the replication of togaviruses in vertebrate and mosquito cells. In Arboviruses in Arthropod Cells In Vitro II53–66 Yunker C. E. Boca Raton: CRC Press;
    [Google Scholar]
  13. Fawcett D. W., Doxsey S., Buscher B. 1981; Salivary gland of the tick vector (R. appendiculatus) of east coast fever. I. Ultrastructure of the type II acinus. Tissue and Cell 3:209–230
    [Google Scholar]
  14. Griffiths G., McDowall A., Bauk R., Dubochet J. 1984; On the preparation of cryosections for immunochemistry. Journal of Ultrastructural Research 89:65–78
    [Google Scholar]
  15. Hart R. J., Beadle D. J., Botham R. P. 1980; On the penetration of ionic lanthanum into the central nervous system of the tick, Amblyomma variegatum . Physiological Entomology 5:401–405
    [Google Scholar]
  16. Hoogstraal H. 1973; Viruses and ticks. In Viruses and Invertebrates349–390 Gibbs A. J. Amsterdam: North-Holland;
    [Google Scholar]
  17. Jones L. D., Davies C. R., Steele G. M., Nuttall P. A. 1988; The rearing and maintenance of ixodid and argasid ticks in the laboratory. Journal of Animal Technology 39:99–106
    [Google Scholar]
  18. Kefalides N. A., Alper R., Clarke C. C. 1979; Biochemistry and metabolism of basement membranes. International Review of Cytology 61:167–228
    [Google Scholar]
  19. Kelly R. O., Dekker R. A. F., Bluemink J. G. 1973; Ligand-mediated osmium binding: its applications in coating biological specimens for scanning electron microscopy. Journal of Ultrastructure Research 45:254
    [Google Scholar]
  20. Kuberski T. T., Rosen L. 1977; A simple technique for the detection of Dengue antigen in mosquitoes by immunofluorescence. American Journal of Tropical Medicine and Hygiene 26:533–537
    [Google Scholar]
  21. Mitchell C. J. 1982; Mosquito vector competence and arboviruses. In Current Topics in Vector Research 163–92 Harris K. F. New York: Praeger;
    [Google Scholar]
  22. Newman G. R., Jasini B., Williams E. D. 1983; A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope. Histochemical Journal 15:543–555
    [Google Scholar]
  23. Nosek J., Korolev M. B., Chuniktin S. P., Kozuch O., Ciampain O. 1984; The replication and eclipse-phase of the tick-borne encephalitis virus in Dermacentor reticulatus . Folia parasitologica 31:187–189
    [Google Scholar]
  24. Obenchain F. D., Galun R. 1982 The Physiology of Ticks Oxford: Pergamon Press;
    [Google Scholar]
  25. Pinto Da Silva P., Parkison C., Dwyer N. 1981; Fracture label: cytochemistry of freeze-fracture faces in the erythrocyte membrane. Proceedings of the National Academy of SciencesU.S.A 78:343–347
    [Google Scholar]
  26. Shope R. E., Sather G. E. 1979; Arboviruses. In Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections767–814 Lennette E. H., Schmidt N. J. Washington, D.C.: American Public Health Association;
    [Google Scholar]
  27. Simons K., Garoff H. 1980; The budding mechanisms of enveloped animal viruses. Journal of General Virology 50:1–21
    [Google Scholar]
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