1887

Abstract

SUMMARY

Seventeen strains of yellow fever virus were compared by immunoblotting using polyclonal and monoclonal antibodies. Strains originating in South America were readily distinguishable from African strains on the basis of differences in the envelope protein. The 17D vaccine strain and strains derived from it differed radically from the parent Asibi and other wild-type strains in that cells infected with 17D did not accumulate intact envelope protein. We suggest that failure to accumulate the major viral surface protein is due to its increased susceptibility to breakdown and that this property may be a contributing factor in the attenuation of the vaccine strains.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-3-557
1989-03-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/3/JV0700030557.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-3-557&mimeType=html&fmt=ahah

References

  1. Buckley A., Gould E. A. 1985; Neutralization of yellow fever virus studied using monoclonal and polyclonal antibodies. Journal of General Virology 66:2523–2531
    [Google Scholar]
  2. Cammack N., Gould E. A. 1986; Antigenic analysis of yellow fever virus glycoproteins: use of monoclonal antibodies in enzyme-linked immunosorbent assays. Journal of Virological Methods 13:135–142
    [Google Scholar]
  3. Clarke D. H. 1960; Antigenic analysis of certain group B arthropod-borne viruses by antibody absorption. Journal of Experimental Medicine 111:21–32
    [Google Scholar]
  4. Deubel V., Digoutte J.-P., Monath T. P., Girard M. 1986; Genetic heterogeneity of yellow fever virus strains from Africa and the Americas. Journal of General Virology 67:209–213
    [Google Scholar]
  5. Deubel V., Schlesinger J. J., Digoutte J.-P., Girard M. 1987; Comparative immunochemical and biological analysis of African and South American yellow fever viruses. Archives of Virology 94:331–338
    [Google Scholar]
  6. Fitzgeorge R., Bradish C. J. 1980; The in vivo differentiation of strains of yellow fever virus in mice. Journal of General Virology 46:1–13
    [Google Scholar]
  7. Gould E. A., Buckley A., Cammack N., Barrett A. D. T., Clegg J. C. S., Ishak R., Varma M. G. R. 1985; Examination of the immunological relationships between the flaviviruses using yellow fever virus monoclonal antibodies. Journal of General Virology 66:1369–1382
    [Google Scholar]
  8. Gould E. A., Buckley A., Groeger B. K., Cane P. A., Doenhoff M. 1987; Immune enhancement of yellow fever virus neurovirulence for mice: studies of mechanisms involved. Journal of General Virology 68:3105–3112
    [Google Scholar]
  9. Hahn C S., Dalrymple J. M., Strauss J. H., Rice C. M. 1987; Comparison of The Virulent Asibi strain of yellow fever virus with the 17D vaccine strain derived from it. Proceedings of the National Academy of SciencesU.S.A 842019–2023
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  11. Monath T. P., Kinney R. M., Schlesinger J. J., Brandriss M. W., Bres P. 1983; Ontogeny of yellow fever 17D vaccine: RNA oligonucleotide fingerprint and monoclonal antibody analyses of vaccines produced worldwide. Journal of General Virology 64:627–637
    [Google Scholar]
  12. Porterfield J. S. 1980; Antigenic characteristics and classification of Togaviridae. In The Togaviruses13–46 Schlesinger R. W. New York & London: Academic Press;
    [Google Scholar]
  13. Schlesinger J. J., Brandriss M. W., Monath T. P. 1983; Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition, and immune precipitation of the virus envelope protein. Virology 125:8–17
    [Google Scholar]
  14. Srivastava A. K., Aira Y., Mori C, Kobayashi Y., Igarashi A. 1987; Antigenicity of Japanese encephalitis virus envelope glycoprotein V 3 (E) and its cyanogen bromide cleaved fragments examined by monoclonal antibodies and Western blotting. Archives of Virology 96:97–107
    [Google Scholar]
  15. Theiler M., Smith H. H. 1937; Effect of prolonged cultivation in vitro upon pathogenicity of yellow fever virus. Journal of Experimental Medicine 65:767–786
    [Google Scholar]
  16. Wengler G., Wengler G., Nowak T., Wahn K. 1987; Analysis of the influence of proteolytic cleavage on the structural organization of the surface of the West Nile flavivirus leads to the isolation of a protease-resistant E protein oligomer from the viral surface. Virology 160:210–219
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-3-557
Loading
/content/journal/jgv/10.1099/0022-1317-70-3-557
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error