1887

Abstract

SUMMARY

We report the isolation of human immunodeficiency virus type 2 (HIV-2) from each of six West Africans with AIDS-related complex or AIDS. One isolate (HIV-2) was molecularly cloned and shown by restriction mapping to differ in seven out of 22 sites from the prototype HIV-2. Nevertheless, by a number of serological criteria these isolates are all clearly HIV-2.

Keyword(s): cloning , HIV-2 and isolates
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-2-479
1989-02-01
2022-08-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/2/JV0700020479.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-2-479&mimeType=html&fmt=ahah

References

  1. Alizon M., Wain-Hobson S., Montagnier L., Sonigo P. 1986; Genetic variability of the AIDS virus: nucleotide sequence analysis of two isolates from African patients.. Cell 46:63–74
    [Google Scholar]
  2. Barré-Sinoussi F., Chermann J. C., Rey F., Nugeyre M. T., Chameret S., Gruest J., Dauguet C., Axler-Blin C. 1983; Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS).. Science 220:868–871
    [Google Scholar]
  3. Benn S., Rutledge R., Folks T., Gold J., Baker L., Mccormick J., Feorino P., Piot P., Quinn T., Martin M. 1985; Genomic heterogeneity of AIDS retroviral isolates from North America and Zaire.. Science 230:949–951
    [Google Scholar]
  4. Blin N., Stafford D. W. 1976; Isolation of high molecular weight DNA.. Nucleic Acids Research 3:2303–2308
    [Google Scholar]
  5. Brown F. 1986; Human immunodeficiency virus.. Science 232:1486
    [Google Scholar]
  6. Clavel F., Guyader M., Guetard D., Sallé M., Montagnier L., Alizon M. 1986; Molecular cloning and polymorphism of the human immune deficiency virus type 2.. Nature, London 324:691–695
    [Google Scholar]
  7. Guyader M., Emerman M., Sonigo P., Clavel F., Montagnier L., Alizon M. 1987; Genome organisation and transactivation of the human immunodeficiency virus type 2.. Nature, London 326:662–669
    [Google Scholar]
  8. Karn J., Matthes H. W., Gait M. J., Brenner S. 1984; A new selective phage cloning vector λ2001, with sites for XbaI, BamHI, HindIII, EcoRI, SstI and XboI.. Gene 32:217–224
    [Google Scholar]
  9. Karpas A. 1983; Unusual virus produced by cultured cells from a patient with AIDS.. Molecular Biology and Medicine 1:457–459
    [Google Scholar]
  10. Karpas A., Gillson W., Bevan P. C., Oates J. K. 1985; Lytic infection by British AIDS virus and development of a rapid cell test for antiviral antibodies.. Lancet ii:695–697
    [Google Scholar]
  11. Karpas A., Hayhoe F. G. J., Hill F., Anderson M., Tenant-Flower M., Howard L., Oates J. K. 1987; Use of Karpas HIV cell test to detect antibodies to HIV-2.. Lancet ii:132–133
    [Google Scholar]
  12. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual382–389 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-2-479
Loading
/content/journal/jgv/10.1099/0022-1317-70-2-479
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error