We found a tandem repeat sequence in the region (designated BS7) in which restriction fragments HI D and I B overlap each other, near the centre of the unique long sequence (U) of the herpes simplex virus type 1 (HSV-1) strain F genome. The I physical map of BS7 was constructed, and the position of a heterogeneous I subfragment from HSV-1 isolates and plaque-purified clones from a single strain was defined on the map. The maximum size difference in the I subfragment was estimated to be 600 bp between these isolates and 100 bp between the clones. The 0.23 kb I subfragment recloned from BS7 was sequenced, and was shown to contain a tandem repeat sequence consisting of 15 units of 12 bp, 5′ TTGGGGCTGGGG 3′. These results suggest that the fragment length heterogeneity in the U of HSV-1 isolates and clones is attributable to copy number variation of the tandem repeat sequence.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error