1887

Abstract

SUMMARY

Groups of mice were immunized intraperitoneally with HEp-2 lysate or respiratory syncytial virus (RSV)-infected HEp-2 cells with or without adjuvant(s). The animals were subsequently challenged intranasally (i.n.) with RSV and/or HEp-2 lysate and studied for RSV shedding in the lung, antigen-specific antibody response and pulmonary histopathology. A significant decrease in virus shedding was detected in each of four groups of animals immunized with a virus preparation and in two of four groups that received a HEp-2 lysate. Mice immunized with adjuvant(s) developed higher antibody-specific responses. All vaccinated animals developed pulmonary histopathology only on subsequent i.n. inoculation with RSV and/or HEp-2 lysate. Denatured extracts of purified RSV, HEp-2, BALB/c lung, cotton rat lung, Buffalo green monkey kidney and human buccal epithelium were tested for reactivity against the sera of immunized mice by an immunoblot method. Sera from all groups of immunized mice reacted with the extracts tested. The data suggest a possible role of reactivity to viral as well as non-viral components in the pathogenesis of RSV vaccine-induced pulmonary inflammation in the mouse model system.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-2-325
1989-02-01
2024-10-09
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/2/JV0700020325.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-2-325&mimeType=html&fmt=ahah

References

  1. Crowell R. L., Field A. K., Schleif W. A., Long W. L., Colonno R. J., Mapoles J. E., Emini E. A. 1985; Monoclonal antibody that inhibits infection of HeLa and rhabdomyosarcoma cells by selected enteroviruses through receptor blockade. Journal of Virology 57:438–445
    [Google Scholar]
  2. Fernie B. F., Cote P. J., Gerin J. L. 1982; Classification of hybridomas to respiratory syncytial virus glycoproteins. Proceedings of the Society for Experimental Biology and Medicine 171:266–271
    [Google Scholar]
  3. Freihorst J., Piedra P. A., Okamoto Y., Ogra P. L. 1988; Effect of respiratory syncytial virus infection on the uptake of and immune response to other inhaled antigens. Proceedings of the Society of Experimental Biology and Medicine 188:191–197
    [Google Scholar]
  4. Fulginiti V. A., Eller J. J., Sieber O. F., Joyner J. W., Minamitani M., Meiklejohn G. 1968; Respiratory virus immunization. I. Field trial of two inactivated respiratory virus vaccines : an aqueous trivalent parainfluenza virus vaccine and an alum-precipitated respiratory syncytial virus vaccine. American Journal of Epidemiology 89:435–448
    [Google Scholar]
  5. Hall C. B., Douglas R. C., Geiman J. M. 1976; Respiratory synctial virus infections in infants. Quantitation and duration of shedding. Journal of Pediatrics 89:11–15
    [Google Scholar]
  6. Hall C. B., Hall W. J., Gala C. L., Magill F. B., Leddy J. P. 1984; Long-term prospective study in children after respiratory virus infection. Journal of Pediatrics 105:358–364
    [Google Scholar]
  7. Henderson F. W., Clyde W. A., Collier A. M., Denny F. W., Senior R. J., Sheaffer C. I., Conley W. G., Christian R. M. 1979a; The etiologic and epidemiologic spectrum of bronchiolitis in pediatrie practice. Journal of Pediatrics 95:183–190
    [Google Scholar]
  8. Henderson F. W., Collier A. M., Clyde W. A., Denny F. W. 1979b; Respiratory syncytial virus infection, reinfection, and immunity: a prospective, longitudinal study in young children. New England Journal of Medicine 300:530–534
    [Google Scholar]
  9. Huang Y. T., Wertz G. W. 1982; The genome of respiratory syncytial virus is a negative stranded RNA that codes for at least seven mRNA species. Journal of Virology 43:150–157
    [Google Scholar]
  10. Kim H. W., Canchola J. G., Brandt C. D., Pyles G., Chanock R. M., Jensen K., Parrott R. H. 1968; Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine. American Journal of Epidemiology 89:422–433
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  12. Lambert D. M., Pons M. W. 1983; Respiratory syncytial virus glycoproteins. Virology 130:204–214
    [Google Scholar]
  13. Lehrer S. B., Nakamura R. M., Tan E. M. 1977; Histopathological changes in mice treated with extracts of the histamine-sensitizing factor of Bordetella pertussis. International Archives of Allergy and Applied Immunology 54:129–142
    [Google Scholar]
  14. Leibovitz E., Freihorst J., Piedra P. A., Ogra P. L. 1988; Modulation of systemic and mucosal immune responses to inhaled ragweed antigen in experimentally induced infection with respiratory syncytial virus implication in virally induced allergy. International Archives of Allergy and Applied Immunology 86:112–116
    [Google Scholar]
  15. Monto A. S., Bryan E. R., Rhodes L. M. 1975; The Tecumseh study of respiratory illness. VII. Further observations on the occurrence of respiratory syncytial virus and Mycoplasma pneumoniae infections. American Journal of Epidemiology 100:458–468
    [Google Scholar]
  16. Prince G. A., Horswood R. L., Berndt J., Suffin S. C., Chanock R. M. 1979; Respiratory syncytial virus infection in inbred mice. Infection and Immunity 26:764–766
    [Google Scholar]
  17. Prince G. A., Jenson A. B., Hemming V. G., Murphy B. R., Walsh E. E., Horswood R. L., Chanock R. M. 1985; Enhancement of respiratory syncytial) virus pulmonary pathology in cotton rats by prior intramuscular inoculation of formalin-inactivated virus. Journal of Virology 57:721–728
    [Google Scholar]
  18. Scott R., Kaul A., Scott M., Chiba Y., Ogra P. L. 1978; Development of in vitro correlation of cell mediated immunity to respiratory syncytial virus infection in man. Journal of Infectious Diseases 137:810–817
    [Google Scholar]
  19. Stott E. J., Taylor G., Ball L. A., Anderson K., Young K. K. Y., King A. M. Q., Wertz G. W. 1987; Immune and histopathological responses in animals vaccinated with recombinant vaccinia viruses that express individual genes of human respiratory syncytial virus. Journal of Virology 61:3855–3861
    [Google Scholar]
  20. Taylor G., Stott E. J., Bew M., Fernie B. F., Cote P. J., Collins A. P., Hughes M., Jebbett J. 1984a; Monoclonal antibodies protect against respiratory syncytial virus infection in mice. Immunology 52:137–142
    [Google Scholar]
  21. Taylor G., Stott E. J., Hughes M., Collins A. P. 1984b; Respiratory syncytial virus infection in mice. Infection and Immunity 43:639–655
    [Google Scholar]
  22. Torre D. C., Quadrelli C., Pugliese A., Maggiolo F. 1986; Effect of betamethasone in lungs of mice treated with lymphocytosis-promoting factor of Bordetella pertussis. Journal of Infectious Diseases 154:238–244
    [Google Scholar]
  23. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of SciencesU.S.A 76:4350–4354
    [Google Scholar]
  24. Ueba O. 1978; Respiratory syncytial virus. I. Concentration and purification of the infectious virus. Acta medicinae Okoyama 32:265–272
    [Google Scholar]
  25. Walsh E. E., Hruska J. 1983; Monoclonal antibodies to respiratory syncytial virus proteins. Identification of the fusion protein. Journal of Virology 47:171–177
    [Google Scholar]
  26. Welliver R. C., Kaul A., Ogra P. L. 1979; Cell-mediated immune response to respiratory syncytial virus infection: relationship to the development of reactive airway disease. Journal of Pediatrics 94:370–375
    [Google Scholar]
  27. Welliver R. C., Kaul T. N., Putnam T. I., Sun M., Riddlesberger K., Ogra P. L. 1980; The antibody response to primary and secondary infection with respiratory syncytial virus in kinetics of class-specific responses. Journal of Pediatrics 96:808–813
    [Google Scholar]
  28. Welliver R. C., Sun M., Rinaldo D., Ogra P. L. 1985; Respiratory syncytial virus-specific IgE responses following infection: evidence for a predominantly mucosal response. Pediatrie Research 19:420–424
    [Google Scholar]
  29. Wong D. T., Rosenband M., Hovey K., Ogra P. L. 1985; Respiratory syncytial virus infection in immunosuppressed animals: implications in human infection. Journal of Medical Virology 17:359–370
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-70-2-325
Loading
/content/journal/jgv/10.1099/0022-1317-70-2-325
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error