1887

Abstract

SUMMARY

This report describes our attempt to establish an experimental animal model for human parainfluenza virus type 4A (HPIV-4A) and 4B (HPIV-4B) infection, which was used to study the immune response to the viruses. Monkeys were inoculated intranasally with the viruses, and at 10 weeks post-infection they were re-infected with homologous subtype viruses. Virus-specific IgM and IgG serum antibodies were measured by ELISA. A small peak of IgM antibody was detected in the monkeys re-infected with HPIV-4B, whereas this response was not detected after re-infection with HPIV-4A. Virus-specific IgA and IgE antibodies were not detected in sera following infection and re-infection with HPIV-4. However virus-specific IgA and IgE antibodies were found in the saliva and nasal exudates of monkeys infected with either HPIV-4A or -4B. Re-infection of monkeys with HPIV-4B also stimulated an IgA and IgE response. To our knowledge this is the first description of a virus-specific IgE antibody response generated by a paramyxovirus infection of an experimental animal. The kinetics of haemagglutinin-inhibition and neutralization (NT) antibodies were similar to that of virus-specific IgG antibodies. The NT titres of sera from HPIV-4A-infected monkeys were enhanced by the addition of complement, whereas complement did not affect the NT activity of sera obtained from HPIV-4B-infected animals. Antigenic specificities of IgG antibody induced by HPIV-4 infection were analysed with radioimmunoprecipitation followed by SDS–PAGE. Anti-NP, -HN and -F antibodies appeared 2 weeks after infection, and the highest titres were found 2 weeks after re-infection. Anti-F antibody production followed a biphasic pattern previously observed in mumps virus infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-12-3487
1989-12-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/12/JV0700123487.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-12-3487&mimeType=html&fmt=ahah

References

  1. Canchola J., Vargosko A. J., Kim H. W., Parrott R. H., Christmas F., Jeffries B., Chanock R. M. 1964; Antigenic variation among newly isolated strains of parainfluenza type 4 virus. American Journal of Hygiene 79:357–364
    [Google Scholar]
  2. Gregg I. 1977; Infection. In Asthma162–176 Clark T. J. H., Godfrey S. London: Chapman and Hall;
    [Google Scholar]
  3. Hishiyama M., Tsurudome M., Ito Y., Yamada A., Sugiura A. 1988; Complement-mediated neutralization test for determination of mumps vaccine-induced antibody. Vaccine 6:423–427
    [Google Scholar]
  4. Ito Y., Tsurudome M., Hishiyama M. 1985; Persistence of mumps virus in mouse L929 cells. Microbiology and Immunology 29:847–857
    [Google Scholar]
  5. Ito Y., Tsurudome M., Hishiyama M. 1987; The polypeptides of human parainfluenza type 2 virus and their synthesis in infected cells. Archives of Virology 95:211–224
    [Google Scholar]
  6. Johnson K. M., Chanock R. M., Cook M. K., Huebner R. J. 1960; Studies of a new human hemadsorption virus. I. Isolation, properties and characterization. American Journal of Hygiene 71:81–92
    [Google Scholar]
  7. Komada H., Tsurudome M., Bando H., Nishio M., Yamada M., Hishiyama M., Ito Y. 1989a; Virus-specific polypeptides of human parainfluenza virus type 4 and their synthesis in infected cells. Virology 171:254–259
    [Google Scholar]
  8. Komada H., Tsurudome M., Ueda M., Nishio M., Bando H., Ito Y. 1989b; Isolation and characterization of monoclonal antibodies to human parainfluenza virus type 4 and their use in revealing antigenic relation between subtype 4A and 4B. Virology 171:28–37
    [Google Scholar]
  9. Merson J. R., Hull R., Estes M. K., Kasel J. A. 1988; Molecular cloning and sequence determination of the fusion protein gene of human parainfluenza virus type 1. Virology 167:97–105
    [Google Scholar]
  10. Rydbeck R., Löve A., Örvell C. 1988; Parainfluenza virus type 2 haemagglutinin-neuraminidase glycoprotein characterized with monoclonal antibodies. Journal of General Virology 69:931–935
    [Google Scholar]
  11. Tsurudome M., Yamada A., Hishiyama M., Ito Y. 1986; Monoclonal antibodies against the glycoproteins of mumps virus: fusion inhibition by anti-HN monoclonal antibody. Journal of General Virology 67:2259–2265
    [Google Scholar]
  12. Tsurudome M., Nishio M., Komada H., Bando H., Ito Y. 1989; Extensive antigenic diversity among human parainfluenza type 2 virus isolates and immunological relationships among paramyxoviruses revealed by monoclonal antibodies. Virology 171:38–48
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-12-3487
Loading
/content/journal/jgv/10.1099/0022-1317-70-12-3487
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error