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Journal of General Virology header logo Journal of General Virology

Volume 70, Issue 11

Serological Prospects for Peptide Vaccines against Foot-and-Mouth Disease Virus No Access

Abstract

Summary

Antibodies to a synthetic peptide corresponding to the 141 to 160 amino acid sequence of the protein VP1 of type O foot-and-mouth disease virus (FMDV) neutralize a wider range of type O isolates than anti-virion serum. Extending this peptide at the amino terminus reduced the number of strains neutralized by the anti-peptide sera. Reactions with antisera to peptides representing non-contiguous native sequences showed that it was also possible to increase the number of strains effectively neutralized. Selected substitutions of a single amino acid at position 148 markedly altered the neutralizing specificity of antibodies elicited by the 141 to 160 peptide. In particular, a peptide with an L → S substitution at this position induced antibodies which neutralized a type O and a type A virus equally, and guinea-pigs inoculated with it were protected from challenge with either virus. Attempts to isolate variant viruses resistant to neutralization with anti-peptide antibody indicated that these occurred at low frequency, and there was some evidence that resistance may be partially conferred by mutations outside the peptide sequence.

  • Received:
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Keyword(s): FMDV , heterotypic cross-protection , neutralizing specificity and peptide vaccines
© Society for General Microbiology 1989
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1989-11-01
2026-04-10

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/content/journal/jgv/10.1099/0022-1317-70-11-2919
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Serological Prospects for Peptide Vaccines against Foot-and-Mouth Disease Virus
J. Gen. Virol. 70, 2919 (1989); https://doi.org/10.1099/0022-1317-70-11-2919
/content/journal/jgv/10.1099/0022-1317-70-11-2919
/content/journal/jgv/10.1099/0022-1317-70-11-2919
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