1887

Abstract

Summary

Competitive binding studies and antigen capture assays were done with monoclonal antibodies (MAbs) raised against hog cholera virus (HCV) to map the corresponding epitopes. A model was constructed in which the 13 epitopes were situated in four distinct antigenic domains: A, B, C and D. Domain A was subdivided into A1, A2 and A3. The functional relevance of this model was assessed by the characterization of pestivirus strains, by neutralization studies with the MAbs, and by isolation of variants that escaped neutralization. The topographical arrangement of the epitopes, as constructed in the model, was corroborated by the functional assays. The MAbs that defined domains A1 and A2 recognized all 94 HCV strains tested. Domains A3, B, C and D varied among the HCV strains. Neutralization was observed with MAbs defining domains A1, B and C. Synergistic neutralization occurred using MAbs against domains A1 and B, and A1 and C, but not within the domains. With MAbs defining A1, B or C, variants could be isolated that escaped neutralization and immunostaining by these MAbs.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-11-2865
1989-11-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/11/JV0700112865.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-11-2865&mimeType=html&fmt=ahah

References

  1. Darbyshire J. H. 1960; A serological relationship between swine fever and mucosal disease of cattle. Veterinary Record 72:331
    [Google Scholar]
  2. Della-Porta A. J., Westaway E. G. 1978; A multi-hit model for the neutralization of animal viruses. Journal of General Virology 38:1–19
    [Google Scholar]
  3. Dinter Z. 1963; Relationship between bovine viral diarrhoea virus and hog cholera virus. Zentralblatt für Bakteriologie , Parasitenkunde , Infektionskrankheiten und Hygiene 188:475–486
    [Google Scholar]
  4. Enzmann P. J., Weiland F. 1978; Structural similarities of hog cholera virus with togaviruses. Archives of Virology 57:339–348
    [Google Scholar]
  5. Heinz F. X., Mandl C., Berger R., Tuma W., Kunz C. 1984; Antibody-induced conformational changes result in enhanced avidity of antibodies to different antigenic sites on the tick-borne encephalitis virus glycoprotein. Virology 133:25–34
    [Google Scholar]
  6. Klaus G. G. B., Pepys M. B., Kitajima K., Askonas B. A. 1979; Activation of mouse complement by different classes of mouse antibody. Immunology 38:687–695
    [Google Scholar]
  7. Laude H. 1979; Nonarbo-Togaviridae: comparative hydrodynamic properties of the Pestivirus genus. Archives of Virology 62:347–352
    [Google Scholar]
  8. Matthaeus W. 1979; Detection of three polypeptides in preparations of bovine viral diarrhea virus. Archives of Virology 59:299–305
    [Google Scholar]
  9. Mengeling W. L., Gutekunst D. E., Fernelius A. L., Pirtle E. C. 1963; Demonstration of an antigenic relationship between hog cholera and bovine viral diarrhea viruses by immunofluorescence. Canadian Journal of Comparative Medicine and Veterinary Science 27:162–164
    [Google Scholar]
  10. Osburn B. I., Clarke G. L., Stewart W. C., Sawyer M. 1973; Border disease-like syndrome in lambs. Antibodies to hog cholera and bovine viral diarrhea viruses. Journal of the American Veterinary Medical Association 153:1165–1167
    [Google Scholar]
  11. Peiris J. S. M., Porterfield J. S., Roehrig J. T. 1982; Monoclonal antibodies against the flavivirus West Nile. Journal of General Virology 58:283–289
    [Google Scholar]
  12. Stone M., Nowinski R. C. 1980; Topological mapping of murine leukemia virus proteins by competition binding assays with monoclonal antibodies. Virology 100:370–381
    [Google Scholar]
  13. Terpstra C., Bloemraad M., Gielkens A. L. J. 1984; The neutralizing peroxidase-linked assay for detection of antibody against swine fever virus. Veterinary Microbiology 9:113–120
    [Google Scholar]
  14. Terpstra C., Wensvoort G. 1988; Natural infections of pigs with bovine viral diarrhoea virus associated with signs resembling swine fever. Research in Veterinary Science 45:137–142
    [Google Scholar]
  15. Thomas A. M., Vrijsen R. A. F., Boeyé A. 1986; Relationship between poliovirus neutralization and aggregation. Journal of Virology 59:479–485
    [Google Scholar]
  16. Van Oirschot J. 1983; Congenital infections with togaviruses. Veterinary Microbiology 8:321–361
    [Google Scholar]
  17. Wensvoort G., Terpstra C. 1986; Heterogeneity of swine fever virus, as defined by monoclonal antibodies. Proceedings , 9th International Congress of Infectious and Parasitic DiseasesMunich 1115–117
    [Google Scholar]
  18. Wensvoort G., Terpstra C., Boonstra J., Bloemraad M., Van Zaane D. 1986; Production of monoclonal antibodies against swine fever and their use in laboratory diagnosis. Veterinary Microbiology 12:101–108
    [Google Scholar]
  19. Wensvoort G., Terpstra C. , De Kluyver E. P. 1989a; Characterization of porcine and some ruminantpestiviruses by cross-neutralization. Veterinary Microbiology in press
    [Google Scholar]
  20. Wensvoort G., Terpstra C., De Kluyver E. P., Kragten C., Warnaar J. 1989b; Antigenic differentiation of pestivirus strains with monoclonal antibodies against hog cholera virus. Veterinary Microbiology in press
    [Google Scholar]
  21. Westaway E. G., Brinton M. A., Gaidamovich S. YA., Horzinek M. C., Igarashi A., Kääriäinen L., Lvov D. K., Porterfield J. S., Russell P. K., Trent D. W. 1985; Togaviridae. Intervirology 24:125–139
    [Google Scholar]
  22. Wilson M. B., Nakane P. K. 1978; Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. In Immunofluorescence and Related Staining Techniques215–224 Knap W., Holubar K., Wick G. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-11-2865
Loading
/content/journal/jgv/10.1099/0022-1317-70-11-2865
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error