1887

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Volume 69, Issue 9

Molecular Properties of Bari 1, a Mild Strain of Cauliflower Mosaic Virus Free

Abstract

Summary

We studied aspects of the structure and expression of the genome of Bari 1, a mild strain of cauliflower mosaic virus. Differences were observed between gene products of Bari 1 detected in inclusion body preparations and those of the more typically severe strain, Cabb B-JI. The most striking difference was the gel mobility of the Bari 1 gene VI polypeptide (apparent 70K) which contrasted with that of Cabb B-JI ( 62K). This difference was also observed between products of translation of viral mRNA suggesting that it was not due to post-translational modification. The open reading frame in the nucleotide sequence of the Bari 1 gene VI region was very similar in size to that of other CaMV strains but corresponded to an amino acid sequence with a much lower overall homology and diverged greatly in a 40 base pair sequence in the 3′ region compared to gene VI sequences of other strains. The level of the Bari 1 aphid transmission polypeptide P18, the product of gene II, was much lower than that of Cabb B-JI. Some of the possible subcellular consequences resulting from the molecular properties of Bari 1 were examined by electron microscopy. Differences were observed in the composition and intactness of Bari 1 cytoplasmic inclusion bodies compared with those of a severe strain, and the presence of nuclear inclusions.

  • Received:
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Keyword(s): CaMV , genome and inclusion bodies
© Society for General Microbiology 1988
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1988-09-01
2024-04-27
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References

  1. AL ANI R., PFEIFFER P., LEBEURIER G. 1979; The structure of cauliflower mosaic virus. II. Identity and location of the viral polypeptides. Virology 93:188–197
     [Google Scholar]
  2. AL ANI R., PFEIFFER P., WHITECHURCH O., LESOT A., LEBEURIER G., HIRTH L. 1980; A virus specified protein produced upon infection by cauliflower mosaic virus. Annales de l’Institut Pasteur/Virologie 131:33–53
     [Google Scholar]
  3. ARMOUR S. L., MELCHER U., PIRONE T. P., LYTTLE D. J., ESSENBERG R. C. 1983; Helper component for aphid transmission encoded by region II of cauliflower mosaic virus DNA. Virology 129:25–30
     [Google Scholar]
  4. BAUGHMAN G. A., JACOBS J. D., HOWELL S. H. 1988; Cauliflower mosaic virus gene VI produces symptomatic phenotype in transgenic tobacco plants. Proceedings of the National Academy of Sciences, U.S.A 85:733–737
     [Google Scholar]
  5. BLAKE M. S., JOHNSTON K. H., RUSSELL-JONES G. J., GOTSCHLICH F. C. 1984; A rapid, sensitive method for detection of alkaline phosphatase-conjugated antibody on Western blots. Analytical Biochemistry 136:175–179
     [Google Scholar]
  6. COVEY S. N. 1985 Organisation and expression of the cauliflower mosaic virus genome. Molecular Plant Virology121–159 Edited by Davies J. W. Boca Raton: CRC Press;
     [Google Scholar]
  7. COVEY S. N., HULL R. 1981; Transcription of cauliflower mosaic virus DNA. Detection of transcripts, properties, and location of the gene encoding the virus inclusion body protein. Virology 111:463–474
     [Google Scholar]
  8. DAUBERT S., RICHINS R., SHEPHERD R. J., GARDNER R. C. 1982; Mapping of the coat protein of cauliflower mosaic virus by its expression in a procaryotic system. Virology 122:444–449
     [Google Scholar]
  9. DÉBORDE D. C, NAEVE C. W., HERLOCHER M. L., MAASSAB H. F. 1986; Resolution of a common RNA sequencing ambiguity by terminal deoxynucleotidyl transferase. Analytical Biochemistry 157:275–282
     [Google Scholar]
  10. DELSENY M., HULL R. 1983; Isolation and characterisation of faithful and altered clones of the genomes of cauliflower mosaic virus isolates Cabb B-JI, CM4-184 and Bari 1. Plasmid 9:31–41
     [Google Scholar]
  11. DEVEREUX J., HAEBERLI P., SMITHIES O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
     [Google Scholar]
  12. DOUGHERTY W. G., HIEBERT E. 1980a; Translation of potyvirus RNA in a rabbit reticulocyte lysate: reaction conditions and identification of capsid protein as one of the products of in vitro translation of tobacco etch and pepper mottle virus RNAs. Virology 101:466–474
     [Google Scholar]
  13. DOUGHERTY W. G., HIEBERT E. 1980b; Translation of potyvirus RNA in a rabbit reticulocyte lysate: identification of nuclear inclusion proteins as products of tobacco etch virus RNA translation and cylindrical inclusion protein as a product of the potyvirus genome. Virology 104:174–182
     [Google Scholar]
  14. FANG R., WU X., BU M., TIAN Y., CAI F., MANG K. 1985; Complete nucleotide sequence of cauliflower mosaic virus (Xinjiang isolate) genomic DNA. Chinese Journal of Virology 1:247–256
     [Google Scholar]
  15. FRANCK A., GUILLEY H., JONARD G., RICHARDS K., HIRTH L. 1980; Nucleotide sequence of cauliflower mosaic virus DNA. Cell 21:285–294
     [Google Scholar]
  16. GARDNER R. C, HOWARTH A. J., HAHN P., BROWN-LEUDI M., SHEPHERD R. J., MESSING J. 1981; The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13 mp7 shotgun sequencing. Nucleic Acids Research 9:2871–2888
     [Google Scholar]
  17. GIBAND M., MESNARD J. M., LEBEURIER G. 1986; The gene III product (P15) of cauliflower mosaic virus is a DNA binding protein while an immunologically related P11 polypeptide is associated with virions. EMBO Journal 5:2433–2438
     [Google Scholar]
  18. GIVORD L., XIONG C, GIBAND M., KOENIG L, HOHN T., LEBEURIER G., HIRTH L. 1984; A second cauliflower mosiac virus product influences the structure of the viral inclusion body. EMBO Journal 3:1423–1427
     [Google Scholar]
  19. GORDON K., PFEIFFER P., FÜTTERER J., HÖHN T. 1988; In vitro expression of cauliflower mosaic virus genes. EMBO Journal 7:309–317
     [Google Scholar]
  20. HAHN P., SHEPHERD R. J. 1982; Evidence of a 58 kilodalton polypeptide as precursor of the coat protein of cauliflower mosaic virus. Virology 116:480–488
     [Google Scholar]
  21. HARKER C, MULLINEAUX P. M., BRYANT J. A., MAULE A. J. 1987; Detection of CaMV gene I and gene VI protein products in vivo using antisera raised to COOH terminal β-galactosidase fusion proteins. Plant Molecular Biology 8:275–287
     [Google Scholar]
  22. HENIKOFF S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
     [Google Scholar]
  23. HOWELL S. H. 1985; The molecular biology of plant DNA viruses. CRC Critical Reviews in Plant Science 2:287–316
     [Google Scholar]
  24. HULL R. 1980; Structure of the cauliflower mosaic virus genome. III. Restriction endonuclease mapping of thirty three isolates. Virology 100:76–90
     [Google Scholar]
  25. HULL R., SADLER J., LONGSTAFF M. 1986; The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses. EMBO Journal 5:3083–3090
     [Google Scholar]
  26. MILLONIG G. 1961; A modified procedure for lead staining of thin sections. Journal of Biophysical and Biochemical Cytology 11:736–739
     [Google Scholar]
  27. MIZUSAWA S., NISHIMURA S., SEELA F. 1986; Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Research 14:1319–1324
     [Google Scholar]
  28. ODELL J. T., HOWELL S. H. 1980; The identification, mapping and characterisation of mRNA for P66, a cauliflower mosaic virus-coded protein. Virology 102:349–359
     [Google Scholar]
  29. PFEIFFER P., HOHN T. 1983; Involvement of reverse transcription in the replication of cauliflower mosaic virus: a detailed model and test of some aspects. Cell 33:781–789
     [Google Scholar]
  30. PLANT A. L., COVEY S. N., GRIERSON D. 1985; Detection of a subgenomic mRNA for gene V, the putative reverse transcriptase gene of cauliflower mosaic virus. Nucleic Acids Research 13:8305–8321
     [Google Scholar]
  31. RICHINS R. D., SCHOLTHOF H. B., SHEPHERD R. J. 1987; Sequence of figwort mosaic virus DNA (caulimovirus group). Nucleic Acids Research 15:8451–8466
     [Google Scholar]
  32. SANGER F., NICKLEN S., COULSON A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
     [Google Scholar]
  33. SCHOELZ J., SHEPHERD R. J. 1988; Host range control of cauliflower mosaic virus. Virology 162:30–37
     [Google Scholar]
  34. SCHOELZ J., SHEPHERD R. J., DAUBERT S. 1986; Region VI of cauliflower mosaic virus encodes a host range determinant. Molecular and Cellular Biology 6:2632–2637
     [Google Scholar]
  35. SHEPHERD R. J. 1981; Cauliflower mosaic virus. CMI/AAB Descriptions of Plant Viruses 243:
     [Google Scholar]
  36. SHOCKEY M. W., GARDNER C. O., MELCHER U., ESSENBERG R. C. 1980; Polypeptides associated with inclusion bodies from leaves of turnips infected with cauliflower mosaic virus. Virology 105:575–581
     [Google Scholar]
  37. STADEN R. 1980; A new computer method for the storage and manipulation of DNA gel reading data. Nucleic Acids Research 8:3673–3694
     [Google Scholar]
  38. STADEN R. 1982; An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Research 10:2951–2961
     [Google Scholar]
  39. THOMAS C. M., HULL R., BRYANT J. A., MAULE A. J. 1985; Isolation of a fraction from cauliflower mosaic virus-infected protoplasts which is active in the synthesis of ( + ) and ( —) strand viral DNA and reverse transcription of primed RNA templates. Nucleic Acids Research 13:4557–4576
     [Google Scholar]
  40. TOWBIN H., STAEHELIN T., GORDON J. 1979; Electrophoretic transfer of proteins from polyacryamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
     [Google Scholar]
  41. WOOLSTON C. J., COVEY S. N., PENSWICK J. R., DAVIES J. W. 1983; Aphid transmission and a polypeptide are specified by a defined region of the cauliflower mosaic virus genome. Gene 23:15–23
     [Google Scholar]
  42. WOOLSTON C. J., CZAPLEWSKI L. G., MARKHAM P. G., GOAD A. S., HULL R., DAVIES J. W. 1987; Location and sequence of a region of cauliflower mosiac virus gene 2 responsible for aphid transmissibility. Virology 160:246–251
     [Google Scholar]
  43. XIONG C, MULLER G., LEBEURIER G., HIRTH L. 1982a; Identification by immunoprecipitation of cauliflower mosaic virus in vitro major translation product with a specific serum against viroplasm protein. EMBO Journal 1:971–976
     [Google Scholar]
  44. XIONG C, BALÀZS E., LEBEURIER G., HINDELANG C, STOECKEL M. E., PORTE A. 1982b; Comparative cytology of two isolates of cauliflower mosaic virus. Journal of General Virology 61:75–81
     [Google Scholar]
  45. XIONG C, LEBEURIER G., HIRTH L. 1984; Detection in vivo of a new gene product (gene III) of cauliflower mosaic virus. Proceedings of the National Academy of Sciences, U.S.A 81:6608–6612
     [Google Scholar]
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Molecular Properties of Bari 1, a Mild Strain of Cauliflower Mosaic Virus
J. Gen. Virol. 69, 2375 (1988); https://doi.org/10.1099/0022-1317-69-9-2375
/content/journal/jgv/10.1099/0022-1317-69-9-2375
/content/journal/jgv/10.1099/0022-1317-69-9-2375
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