1887

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Volume 69, Issue 9

Molecular Characterization of Sorghum Chlorotic Spot Virus, a Proposed Furovirus Free

Abstract

Summary

A virus morphologically and physicochemically similar to the type member of the furovirus group, wheat soil-borne mosaic virus (WSBMV), has been isolated from and partially characterized. The virus, sorghum chlorotic spot virus (SCSV), causes symptoms which include distinct elongated chlorotic spots and ring spots as well as yellowing on systemically infected leaves of sorghum and inbred lines. SCSV is mechanically transmissible to and produces symptoms on the inoculated leaves of and . The virus is bipartite with two distinct rods, 20 nm in diameter and 260 and 140 nm in length. Virions are composed of a single 20·5K capsid protein and two non-homologous, non-polyadenylated, genomic RNAs of approx. 6·2 kb ( 2·2 × 10) for RNA-1 and 3·5 kb ( 1·2 × 10) for RNA-2. SCSV and WSBMV capsid proteins are serologically related as determined by Western blot and immunogold cytochemical analysis. Northern blot hybridizations indicated that there is no homology between SCSV RNA and WSBMV RNA under high stringency conditions. Unfractionated SCSV RNAs direct the synthesis of 180K, 170K, 110K, 50K, 42K, 25K and 20.5K polypeptides . The 110K, 25K and 20.5K products are immunoprecipitated by antiserum raised against SCSV capsid protein. Comparative translation analysis with WSBMV suggests that the SCSV capsid protein cistron resides on the 5′ terminus of RNA-2. A 1·8 kb cDNA clone was synthesized using SCSV RNA-2. T7 transcripts from this clone directed the synthesis of several polypeptides, none of which was immunoprecipitated by antiserum to the capsid protein. SCSV is similar to, but distinct from, WSBMV and is proposed to be a new member of the furovirus group.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): furovirus , SCSV and WSBMV
© Society for General Microbiology 1988
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1988-09-01
2023-10-03
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Molecular Characterization of Sorghum Chlorotic Spot Virus, a Proposed Furovirus
J. Gen. Virol. 69, 2335 (1988); https://doi.org/10.1099/0022-1317-69-9-2335
/content/journal/jgv/10.1099/0022-1317-69-9-2335
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