1887

Abstract

Summary

A virus morphologically and physicochemically similar to the type member of the furovirus group, wheat soil-borne mosaic virus (WSBMV), has been isolated from and partially characterized. The virus, sorghum chlorotic spot virus (SCSV), causes symptoms which include distinct elongated chlorotic spots and ring spots as well as yellowing on systemically infected leaves of sorghum and inbred lines. SCSV is mechanically transmissible to and produces symptoms on the inoculated leaves of and . The virus is bipartite with two distinct rods, 20 nm in diameter and 260 and 140 nm in length. Virions are composed of a single 20·5K capsid protein and two non-homologous, non-polyadenylated, genomic RNAs of approx. 6·2 kb ( 2·2 × 10) for RNA-1 and 3·5 kb ( 1·2 × 10) for RNA-2. SCSV and WSBMV capsid proteins are serologically related as determined by Western blot and immunogold cytochemical analysis. Northern blot hybridizations indicated that there is no homology between SCSV RNA and WSBMV RNA under high stringency conditions. Unfractionated SCSV RNAs direct the synthesis of 180K, 170K, 110K, 50K, 42K, 25K and 20.5K polypeptides . The 110K, 25K and 20.5K products are immunoprecipitated by antiserum raised against SCSV capsid protein. Comparative translation analysis with WSBMV suggests that the SCSV capsid protein cistron resides on the 5′ terminus of RNA-2. A 1·8 kb cDNA clone was synthesized using SCSV RNA-2. T7 transcripts from this clone directed the synthesis of several polypeptides, none of which was immunoprecipitated by antiserum to the capsid protein. SCSV is similar to, but distinct from, WSBMV and is proposed to be a new member of the furovirus group.

Keyword(s): furovirus , SCSV and WSBMV
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/content/journal/jgv/10.1099/0022-1317-69-9-2335
1988-09-01
2022-01-25
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References

  1. BIRNBOIM H. C., DOLY J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  2. BOUZOUBAA S., GUILLEY H., JONARD G., RICHARDS K., PUTZ C. 1985; Nucleotide sequence analysis of RNA-3 and RNA-4 of beet necrotic yellow vein virus, isolates F2 and G1. Journal of General Virology 66:1553–1564
    [Google Scholar]
  3. BOUZOUBAA S., ZIEGLER V., BECK D., GUILLEY H., RICHARDS K., JONARD G. 1986; Nucleotide sequence of beet necrotic yellow vein virus RNA-2. Journal of General Virology 67:1689–1700
    [Google Scholar]
  4. BOUZOUBAA S., QUILLET L., GUILLEY H., JONARD G., RICHARDS K. 1987; Nucleotide sequence of beet necrotic yellow vein virus RNA-1. Journal of General Virology 68:615–626
    [Google Scholar]
  5. BRAKKE M. K. 1977; Sedimentation coefficients of the virions of soil-borne wheat mosaic virus. Phytopathology 67:1433–1438
    [Google Scholar]
  6. BRAKKE M. K., RAO S. A. 1967; Maintenance of soilborne wheat mosaic virus cultures by transfer through root washings. Plant Disease Reporter 51:1005–1008
    [Google Scholar]
  7. BRAKKE M. K., ESTES A. P., SCHUSTER M. L. 1965; Transmission of soil-borne wheat mosaic virus. Phytopathology 55:79–86
    [Google Scholar]
  8. BRUNT A. A., STACE-SMITH R. 1978; Some hosts, properties and possible affinities of a labile virus from Hypochoeris radicata (Compositae). Annals of Applied Biology 90:205–214
    [Google Scholar]
  9. CARRINGTON J. C., MORRIS T. J. 1984; Complementary DNA cloning and analysis of carnation mottle virus RNA. Virology 139:22–31
    [Google Scholar]
  10. DOUGHERTY W. G., HIEBERT E. 1980; Translation of potyvirus RNA in a rabbit reticulocyte lysate: cell-free translation strategy and a genetic map of the potyviral genome. Virology 104:183–194
    [Google Scholar]
  11. EL MANNA M. M., BRUENING G. 1973; Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus. Virology 56:198–206
    [Google Scholar]
  12. ESTES A. P., BRAKKE M. K. 1966; Correlation of Polymyxa graminis with transmission of soil-borne wheat mosaic virus. Virology 28:772–774
    [Google Scholar]
  13. FUJISAWA I., SUGIMOTO T. 1976; Transmission of beet necrotic yellow vein virus by Polymyxa betae. Annals of the Phytopathological Society of Japan 43:583–586
    [Google Scholar]
  14. GUBLER U., HOFFMAN B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  15. GUMPF D. J. 1971; Purification and properties of soil-borne wheat mosaic virus. Virology 43:588–596
    [Google Scholar]
  16. HARRISON B. D. 1974; Potato mop-top virus. CMI/AAB Descriptions of Plant Viruses 138:
    [Google Scholar]
  17. HIEBERT E., PURCIFULL D. E. 1981; Mapping of the two coat protein genes on the middle RNA component of squash mosaic virus (Comovirus group). Virology 113:630–636
    [Google Scholar]
  18. HSU Y. H., BRAKKE M. K. 1985; Cell-free translation of soil-borne wheat mosaic virus RNAs. Virology 143:272–279
    [Google Scholar]
  19. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  20. LANGENBERG W. G. 1985; Immunoelectron microscopy of wheat spindle streak and soil-borne wheat mosaic doubly infected wheat. Journal of Ultrastructure Research 92:72–79
    [Google Scholar]
  21. LANGENBERG W. G. 1986; Deterioration of several rod-shaped wheat viruses following antibody decoration. Phytopathology 76:339–341
    [Google Scholar]
  22. LEACH J. E., RAMUNDO B. A., PEARSON D. L., CLAFLIN L. E. 1987; Dot-immunobinding assay for detecting Xanthomonas campestris pv. holcicola in sorghum. Plant Disease 71:30–33
    [Google Scholar]
  23. LEMAIRE O., MERDINOGLU D., VALENTIN P., PUTZ C, ZIEGLER-GRAFF V., GUILLEY H., IONARD G., RICHARDS K. 1988; Effect of beet necrotic yellow vein virus RNA composition on transmission by Polymyxa betae. Virology 162:232–235
    [Google Scholar]
  24. LIN N.-S., LANGENBERG W. G. 1983; Immunohistochemical localization of barley stripe mosaic virions in infected wheat cells. Journal of Ultrastructure Research 84:16–23
    [Google Scholar]
  25. LOMMEL S. A., MCCAIN A. H., MORRIS T. J. 1982; Evaluation of indirect ELISA for the detection of plant viruses. Phytopathology 72:1018–1022
    [Google Scholar]
  26. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. MAYO M. A., REDDY D. V. R. 1985; Translation products of RNA from Indian peanut clump virus. Journal of General Virology 66:1347–1351
    [Google Scholar]
  28. MORRIS T. J., HESS P. T., PINNOCK D. E. 1979; Physicochemical characterization of a small RNA virus associated with baculovirus infection in Trichoplusia ni. Intervirology 11:238–247
    [Google Scholar]
  29. PELHAM H. R. B., JACKSON R. J. 1976; An efficient mRNA-dependent translation system from reticulocyte lysate. European Journal of Biochemistry 67:247–256
    [Google Scholar]
  30. PUTZ C. 1977; Composition and structure of beet necrotic yellow vein virus. Journal of General Virology 35:397–401
    [Google Scholar]
  31. PUTZ C, PINCK L., PINCK M., FRITSCH C. 1983; Identification of 3′ and 5′ ends of beet necrotic yellow vein virus RNAs. Presence of poly A sequences. FEBS Letters 156:41–56
    [Google Scholar]
  32. RANDLES J. W., HARRISON B. D., ROBERTS I. M. 1976; Nicotiana velutina mosaic virus: purification, properties and affinities with other rod-shaped viruses. Annals of Applied Biology 84:193–204
    [Google Scholar]
  33. RAO A. S., BRAKKE M. K. 1969; Relation of soil-borne wheat mosaic virus and its fungal vector, Polymyxa graminis. Phytopathology 59:581–587
    [Google Scholar]
  34. RAVE N., CRKVENIAKOU R., BOEDTKER H. 1979; Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels. Nucleic Acids Research 6:3559–3567
    [Google Scholar]
  35. REDDY D. V. R., RAJESHWARI R., IIZUKA N., LESEMANN D. E., NOLT B. L., GOTO T. 1983; The occurrence of Indian peanut clump, a soil-borne virus disease of groundnuts (Arachis hypogaea) in India. Annals of Applied Biology 102:305–310
    [Google Scholar]
  36. RICHARDS K., JONARD G., GUILLEY H., ZIEGLER V., PUTZ C. 1985; In vitro translation of beet necrotic yellow vein virus RNA and studies of sequence homology among the RNA species using cloned cDNA probes. Journal of General Virology 66:345–350
    [Google Scholar]
  37. ROBERTS I. M., HARRISON B. D. 1979; Detection of potato leafroll and potato mop-top viruses by immunosorbent electron microscopy. Annals of Applied Biology 93:289–297
    [Google Scholar]
  38. SHIRAKO Y., BRAKKE M. K. 1984a; Two purified RNAs of soil-borne wheat mosaic virus are needed for infection. Journal of General Virology 65:119–127
    [Google Scholar]
  39. SHIRAKO Y., BRAKKE M. K. 1984b; Spontaneous deletion mutation of soil-borne wheat mosaic virus RNA II. Journal of General Virology 65:855–858
    [Google Scholar]
  40. SHIRAKO Y., EHARA Y. 1986; Comparison of the in vitro translation products of wild-type and a deletion mutant of soil-borne wheat mosaic virus. Journal of General Virology 67:1237–1245
    [Google Scholar]
  41. TAMADA T. 1975; Beet necrotic yellow vein virus. CMI/AAB Descriptions of Plant Viruses 144:
    [Google Scholar]
  42. THOUVENEL J. C, FAUQUET C. 1981; Further properties of peanut clump virus and studies on its natural transmission. Annals of Applied Biology 97:99–107
    [Google Scholar]
  43. THOUVENEL J. C, DOLLET M., FAUQUET C. 1976; Some properties of peanut clump, a newly discovered virus. Annals of Applied Biology 84:311–320
    [Google Scholar]
  44. TOWBIN H., STAEHELIN T., GORDON J. 1979; Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  45. TSUCHIZAKI T., HIBINO H., SAITO Y. 1975; The biological functions of short and long particles of soil-borne wheat mosaic virus. Phytopathology 65:523–532
    [Google Scholar]
  46. ZIEGLER V., RICHARDS K., GUILLEY H., JONARD G., PUTZ C. 1985; Cell-free translation of beet necrotic yellow vein virus: readthrough of the coat protein cistron. Journal of General Virology 66:2079–2087
    [Google Scholar]
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