1887

Abstract

Summary

Purified DNAs from Marek’s disease virus (MDV) and the herpesvirus of turkeys (HVT) were randomly sheared and cloned into the M13 bacteriophage. Two-hundred and ten MDV and 130 HVT clones were sequenced to give representative samples of the genome sequences. The predicted amino acid sequences from these gammaherpesviruses were compared to known sequences from other herpesviruses using computer analysis. Thirty-five MDV and 24 HVT genes were identified by comparison with varicella-zoster virus (VZV), an alphaherpesvirus. However, only 14 MDV and seven HVT genes, giving generally lower homology scores, were found by comparison with Epstein-Barr virus (EBV), a gammaherpesvirus, indicating that MDV and HVT sequences bear greater similarity to VZV than to EBV sequences. A number of sequences were mapped by hybridizing labelled M13 clones to Southern blots of restriction fragments of MDV or HVT DNA. The results were consistent with the MDV and HVT genomes being collinear with VZV.

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1988-08-01
2024-11-05
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References

  1. BAER R., BANKIER A. T., BIGGIN M. D., DEININGER P. L., FARRELL P. J., GIBSON T. J., HATFULL G., HUDSON G. S., SATCHWELL S. C, SÉGUIN C, TUFFNELL P. S., BARRELL B. G. 1984; DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature, London 310:207–211
    [Google Scholar]
  2. BIGGIN M. D., GIBSON T. J., HONG G. F. 1983; Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences, U.S.A 80:3963–3965
    [Google Scholar]
  3. BINNS M. M., STENZLER L., TOMLEY F. M., CAMPBELL J., BOURSNELL M. E. G. 1987; Identification by a random sequencing strategy of the fowlpox virus DNA polymerase gene, its nucleotide sequence and comparison with viral polymerases. Nucleic Acids Research 15:6563–6573
    [Google Scholar]
  4. BZIK D. J., FOX B. A., DELUCA N. A., PERSON S. 1984; Nucleotide sequence specifying the glycoprotein gene, gB, of herpes simplex virus type 1. Virology 133:301–314
    [Google Scholar]
  5. CAMERON K. R., STAMMINGER T., CRAXTON M., BODMER W., HONESS R. W., FLECKENSTEIN B. 1987; The 160,000-Mr virion protein encoded at the right end of the herpesvirus saimiri genome is homologous to the 140,000-M, membrane antigen encoded at the left end of the Epstein-Barr virus genome. Journal of Virology 61:2063–2070
    [Google Scholar]
  6. CEBRIAN I., KASCHA-DIERICH C, BERTHELOT N., SHELDRICK P. 1982; Inverted repeat nucleotide sequences in the genomes of Marek’s disease virus and the herpesvirus of the turkey. Proceedings of the National Academy of Sciences, U.S.A 79:555–558
    [Google Scholar]
  7. CHURCHILL A. E., CHUBB R. C., BAXENDALE W. 1969; The attenuation with loss of oncogenicity of the herpes-type virus of Marek’s disease (strain hprs-16) on passage in cell culture. Journal of General Virology 4:557–564
    [Google Scholar]
  8. CRANAGE M. P., KOUZARIDES T., BANKIER A. T., SATCHWELL S., WATSON K., TOMLINSON P., BARRELL B., HART H., BELL S. E., MINSON A. C., SMITH G. H. 1986; Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralising antibodies via its expression in recombinant vaccinia virus. EMBO Journal 5:3057–3063
    [Google Scholar]
  9. CRANAGE M. P., SMITH G. L., BELL S. E., HART H., BROWN C, BANKIER A. T., TOMLINSON P., BARRELL B. G., MINSON T. C. 1988; Identification and expression of a human cytomegalovirus glycoprotein with homology to the Epstein-Barr virus BXLF2 product, varicella-zoster virus gpIII, and herpes simplex virus type 1 glycoprotein H. Journal of Virology 62:1416–1422
    [Google Scholar]
  10. DAVISON A. J. 1984; Structure of the genome termini of varicella-zoster virus. Journal of General Virology 65:1969–1977
    [Google Scholar]
  11. DAVISON A. J., MCGEOCH D. J. 1986; Evolutionary comparisons of the S segments in the genomes of herpes simplex virus type 1 and varicella-zoster virus. Journal of General Virology 67:597–611
    [Google Scholar]
  12. DAVISON A. J., SCOTT J. E. 1986a; The complete DNA sequence of varicella-zoster virus. Journal of General Virology 67:1759–1816
    [Google Scholar]
  13. DAVISON A. J., SCOTT J. E. 1986b; DNA sequence of the major capsid protein gene of herpes simplex virus type 1. Journal of General Virology 67:2279–2286
    [Google Scholar]
  14. DAVISON A. J., TAYLOR P. 1987; Genetic relations between varicella-zoster virus and Epstein-Barr virus. Journal of General Virology 68:1067–1079
    [Google Scholar]
  15. DAVISON A. J., WILKIE N. M. 1983; Location and orientation of homologous sequences in the genomes of five herpesviruses. Journal of General Virology 64:1927–1942
    [Google Scholar]
  16. DEININGER P. L. 1983; Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis. Analytical Biochemistry 129:216–223
    [Google Scholar]
  17. DRAPER K. G., COSTA R. H., LEE G. T-Y, SPEAR P. G., WAGNER E. K. 1984; Molecular basis of the glycoprotein-C-negative phenotype of herpes simplex virus type 1 macroplaque strain. Journal of Virology 51:578–585
    [Google Scholar]
  18. FRAME M. C, PURVES F. C, MCGEOCH D. J., MARSDEN H. S., LEADER D. P. 1987; Identification of the herpes simplex virus protein kinase as the product of viral gene US3. Journal of General Virology 68:2699–2704
    [Google Scholar]
  19. FUKUCHI K., SUDO M., LEE Y-S., TANAKA A., NONOΥAMA M. 1984; Structure of Marek’s disease virus DNA: detailed restriction enzyme map. Journal of Virology 51:102–109
    [Google Scholar]
  20. GOMPELS U., MINSON A. 1986; The properties and sequence of glycoprotein H of the herpes simplex type 1. Virology 153:230–247
    [Google Scholar]
  21. GOMPELS A. A., CRAXTON M. A., HONESS R. W. 1988; Conservation of gene organization in the lymphotropic herpesviruses herpesvirus saimiri and Epstein-Barr virus. Journal of Virology 62:757–767
    [Google Scholar]
  22. HIRT B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  23. IGARASHI T., TAKAHASHI M., DONOVAN J., JESSIP J., SMITH M., HIRAI K., TANAKA A., NONOΥAMA M. 1987; Restriction enzyme map of herpesvirus of turkey DNA and its colinear relationship with Marek’s disease virus DNA. Virology 157:351–358
    [Google Scholar]
  24. KOUZARIDES T., BANKIER A. T., SATCHWELL S. C, WESTON K., TOMLINSON P., BARRELL B. G. 1987; Sequence and transcription analysis of the human cytomegalovirus DNA polymerase gene. Journal of Virology 61:125–133
    [Google Scholar]
  25. LEE Y. S., TANAKA A., LAU R. Y., NONOYAMA M., RABIN H. 1980; Comparative studies of herpesvirus papio (baboon herpesvirus) DNA and Epstein-Barr virus DNA. Journal of General Virology 51:245–253
    [Google Scholar]
  26. LIPMAN D. J., PEARSON W. R. 1985; Rapid and sensitive protein similarity searches. Science 227:1435–1441
    [Google Scholar]
  27. MCGEOCH D. J., DAVISON A. J. 1986a; DNA sequence of the herpes simplex virus type 1 gene encoding glycoprotein gH, and identification of homologues in the genomes of varicella-zoster virus and Epstein-Barr virus. Nucleic Acids Research 14:4281–4292
    [Google Scholar]
  28. MCGEOCH D. J., DAVISON A. J. 1986b; Alphaherpesviruses possess a gene homologous to the protein kinase gene family of eukaryotes and retroviruses. Nucleic Acids Research 14:1765–1777
    [Google Scholar]
  29. MCGEOCH D. J., DOLAN A., DONALD S., RIXON F. J. 1985; Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. Journal of Molecular Biology 181:1–13
    [Google Scholar]
  30. MCGEOCH D. J., DOLAN A., FRAME M. C. 1986; DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes. Nucleic Acids Research 14:3435–3448
    [Google Scholar]
  31. PAYNE L. N. 1982 Biology of Marek’s disease virus and the herpesvirus of turkeys. The Herpesviruses 1347–431 Edited by Roizman B. New York & London: Plenum Press;
    [Google Scholar]
  32. PELLETT P. E., BIGGIN M. D., BARRELL B., ROIZMAN B. 1985; Epstein-Barr virus genome may encode a protein showing significant amino acid and predicted secondary structure homology with glycoprotein B of herpes simplex virus 1. Journal of Virology 56:807–813
    [Google Scholar]
  33. PURCHASE H. G., BIGGS P. M. 1967; Characterization of five isolates of Marek’s disease. Research in Veterinary Science 8:440–449
    [Google Scholar]
  34. PURCHASE H. G., OKAZAKI W., BURMESTER B. R. 1972; Long term field trials with herpesvirus of turkeys vaccine against Marek’s disease. Avian Pathology 16:57–71
    [Google Scholar]
  35. QUINN J. P., MCGEOCH D. J. 1985; DNA sequence of the region in the genome of herpes simplex virus type 1 containing the genes for DNA polymerase and the major DNA binding protein. Nucleic Acids Research 13:8143–8163
    [Google Scholar]
  36. ROBBINS A. K., DORNEY D. J., WATHEN M. W., WHEALY M. E., GOLD C, WATSON R. J., HOLLAND L. E., WEED S. D., LEVINE M., GLORIOSO J. C., ENQUIST L. W. 1987; The Pseudorabies virus gll gene is closely related to the gB glycoprotein gene of herpes simplex virus. Journal of Virology 61:2691–2701
    [Google Scholar]
  37. ROIZMAN B. 1982 The family herpesviridae: general description, taxonomy, and classification. The Herpesviruses 1347–431 Edited by Roizman B. New York & London: Plenum Press;
    [Google Scholar]
  38. ROIZMAN B., CARMICHAEL L. E., DEINHARDT F., DE-THÉ G., NAHMIAS A. J., PLOWRIGHT W., RAPP F., SHELDRICK P., TAKAHASHI M., WOLF K. 1981; Herpesviridae. Definition, provisional nomenclature and taxonomy. Intervirology 16:201–217
    [Google Scholar]
  39. ROSS L. J. N. 1985 Molecular biology of the virus. Marek’s Disease113–150 Edited by Payne L. N. The Hague: Martinus Nijhoff;
    [Google Scholar]
  40. ROSS L. J. N., FRAZIER J. A., BIGGS P. M. 1971 An antigen common to some avian and mammalian herpesviruses. Oncogenesis and Herpesviruses480–484 Edited by Biggs P. M., de-ThÉ G., Payne L. N. Lyon: WHO International Agency for Research on Cancer;
    [Google Scholar]
  41. ROSS L. J. N., DE LORBE W., VARMUS H. E., BISHOP J. M., BRAHIC M., HAASE A. 1981; Persistence and expression of Marek’s disease virus DNA in tumour cells and peripheral nerves studied by in situ hybridization. Journal of General Virology 57:285–296
    [Google Scholar]
  42. VAN ZAANE D., BRINKHOF J. M. A., WESTENBRINK F., GIELKENS A. L. J. 1982; Molecular biological characterization of Marek’s disease virus. II. Differentiation of various MDV and HVT strains. Virology 121:133–146
    [Google Scholar]
  43. VELICER L. F., YOGER D. R., CLARK J. L. 1978; Marek’s disease herpesviruses. III. Purification and characterization of Marek’s disease herpesvirus B antigen. Journal of Virology 27:205–217
    [Google Scholar]
  44. WITTER R. L., NAZERIAN K., PURCHASE H. G., BURGOYNE G. H. 1970; Isolation from turkeys of cell-associated herpesvirus antigenically related to Marek’s disease virus. American Journal of Veterinary Research 31:525–538
    [Google Scholar]
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