1887

Journal of General Virology header logo

Volume 69, Issue 8

The Subcellular Location of the Gene 1 Product of Cauliflower Mosaic Virus Is Consistent with a Function Associated with Virus Spread Free

Abstract

Summary

Using immunogold cytochemistry, plasmodesmata have been identified as subcellular locations of the protein product (P1) of cauliflower mosaic virus gene 1 in infected turnip tissue. Thin sections, from tissue adjacent to chlorotic local lesions on systemically infected fully expanded leaves, were probed with antiserum to a protein product derived from a -gene 1 fusion, and with control sera. Modified plasmodesmata between infected mesophyll cells, and plasmodesmata in the end walls of phloem parenchyma cells were specifically labelled with anti-P1 serum. In the former case, the position of the label suggested that P1 was extracellular and had formed a structural component of the modified plasmodesmata. Cell walls at the corners of cells in both healthy and infected tissue were also labelled. Anti-P1 serum also reacted with nuclei and the leaf cuticle, in both healthy and infected tissue. These observations provide strong circumstantial evidence that P1 is involved in the cell-to-cell movement of cauliflower mosaic virus.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CaMV , plasmodesmata and virus spread
© Society for General Microbiology 1988
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-8-1809
1988-08-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/8/JV0690081809.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-8-1809&mimeType=html&fmt=ahah

References

  1. ATABEKOV J. G., DOROKHOV Y. L. 1984; Plant virus-specific transport function and resistance of plants to viruses. Advances in Virus Research 29:313–364
     [Google Scholar]
  2. BASSI M., FAVALI M. A., CONTI G. G. 1974; Cell wall protrusions induced by cauliflower mosaic virus in Chinese cabbage leaves: a cytochemical and autoradiographic study. Virology 60:353–358
     [Google Scholar]
  3. CONTI G. G., VEGETTI G., BASSI M., FAVALI M. 1972; Some ultrastructural and cytochemical observations on Chinese cabbage infected with cauliflower mosaic virus. Virology 47:694–700
     [Google Scholar]
  4. DEOM C. M., OLIVER M. J., BEACHY R. N. 1987; The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement. Science 237:389–394
     [Google Scholar]
  5. HARKER C. L., MULLINEAUX P. M., BRYANT J. A., MAULE A. J. 1987; Detection of CaMV gene 1 and gene VI products in vivo using antisera raised to COOH-terminal β-galactosidase fusion proteins. Plant Molecular Biology 8:275–287
     [Google Scholar]
  6. HULL R., COVEY S. N. 1985; Cauliflower mosaic virus: pathways to infection. BioEssays 3:160–163
     [Google Scholar]
  7. HULL R., SHEPHERD R. J., HARVEY J. D. 1976; Cauliflower mosaic virus: an improved purification procedure and some properties of the virus particles. Journal of General Virology 31:93–100
     [Google Scholar]
  8. HULL R., SADLER J., LONGSTAFF M. 1986; The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses. EMBO Journal 5:3083–3090
     [Google Scholar]
  9. KITAJIMA E. W., LAURITIS J. A. 1969; Plant virions in plasmodesmata. Virology 37:681–685
     [Google Scholar]
  10. LAEMMLI V. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
     [Google Scholar]
  11. LAWSON R. H., HEARON S. S. 1974; Ultrastructure of carnation etched ring virus infected Saponaria vaccaria and Dianthus caryophyllus. Journal of Ultrastructure Research 48:201–215
     [Google Scholar]
  12. LEONARD D. L., ZAITLIN M. 1982; A temperature-sensitive strain of tobacco mosaic virus defective in cell-to-cell movement generates an altered viral-coded protein. Virology 117:416–424
     [Google Scholar]
  13. LISTER R. M. 1966; Possible relationships of virus-specific products of tobacco rattle virus infections. Virology 28:350–353
     [Google Scholar]
  14. MARTINEZ-IZQUIERDO J. A., FUTTERER J., HÖHN T. 1987; Protein encoded by ORF 1 of cauliflower mosaic virus is part of the inclusion body. Virology 160:527–530
     [Google Scholar]
  15. MENISSIER-DE MURCIA F., GELDREICH A., LEBEURIER G. 1986; Evidence for a protein kinase activity associated with purified particles of cauliflower mosaic virus. Journal of General Virology 67:1885–1891
     [Google Scholar]
  16. MESHI T., WATANABE Y., SAITO T., SUGIMOTO A., MAEDA T., OKADA Y. 1987; Function of the 30Kd protein of tobacco mosaic virus: involvement in cell-to-cell movement and dispensability for replication. EMBO Journal 6:2557–2563
     [Google Scholar]
  17. PLASKITT K. A., WATKINS P. A. C, SLEAT D. E., GALLIE D. R., SHAW J. G., WILSON T. M. A. 1988; Immunogold labeling locates the site of disassembly and transient gene expression of tobacco mosaic virus-like pseudovirus particles in vivo. Molecular Plant-Microbe Interactions 1:10–16
     [Google Scholar]
  18. REYNOLDS E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
     [Google Scholar]
  19. RICHINS R. D., SCHLOTHOF H. B., SHEPHERD R. T. 1987; Sequence of figwort mosaic virus DNA (caulimovirus group). Nucleic Acids Research 15:8451–8466
     [Google Scholar]
  20. STANLEY J., TOWNSEND R. 1986; Infectious mutants of cassava latent virus generated in vivo from intact recombinant DNA containing single copies of the genome. Nucleic Acids Research 14:5981–5988
     [Google Scholar]
  21. STANLEY K. K. 1983; Solubilisation and immune-detection of β-galactosidase hybrid proteins carrying foreign antigenic determinants. Nucleic Acids Research 11:4077–4092
     [Google Scholar]
  22. STUSSI-GARAUD C, GARAUD J.-C, BERNA A., GODEFROY-COLBURN T. 1987; In situ location of an alfalfa mosaic virus non-structural protein in plant cell walls: correlation with virus transport. Journal of General Virology 68:1779–1784
     [Google Scholar]
  23. TALIANSKY M. E., MALYSHENKO S. I., PSHENNIKOVA E. S., KAPLAN I. B., ULANOVA E. F., ATABEKOV J. G. 1982; Plant virus-specific transport function: virus genetic control required for systemic spread. Virology 122:318–326
     [Google Scholar]
  24. TOMENLUS K., CLAPHAM D., MESHI T. 1987; Localization by immunogold cytochemistry of the virus-coded 30K protein in plasmodesmata of leaves infected with tobacco mosaic virus. Virology 160:363–371
     [Google Scholar]
  25. WATANABE Y., OOSHIKA I., MESHI T., OKADA Y. 1986; Subcellular localization of the 30K protein in TMV-inoculated tobacco protoplasts. Virology 152:414–420
     [Google Scholar]
  26. XIONG C, MÜLLER S., LEBEURIER G., HIRTH L. 1982; Identification by immunoprecipitation of cauliflower mosaic virus in vitro major translation product with a specific serum against viroplasm protein. EMBO Journal 1:971–976
     [Google Scholar]
  27. YOUNG M. J., DAUBERT S. D., SHEPHERD R. J. 1987; Gene 1 products of cauliflower mosaic virus detected in extracts of infected tissue. Virology 158:444–446
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-8-1809
Loading
The Subcellular Location of the Gene 1 Product of Cauliflower Mosaic Virus Is Consistent with a Function Associated with Virus Spread
J. Gen. Virol. 69, 1809 (1988); https://doi.org/10.1099/0022-1317-69-8-1809
/content/journal/jgv/10.1099/0022-1317-69-8-1809
/content/journal/jgv/10.1099/0022-1317-69-8-1809
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error