The genomes of four closely related variants of granulosis virus, isolated in England and New Zealand, have been mapped for the restriction endonucleases RI, HI, dIII, I, I, I, I, and I by secondary digestion of isolated restriction fragments. A total of 69, 70 or 71 fragments were unambiguously mapped for these isolates. A modified system of fragment lettering was adopted so that, for each enzyme, the same letter could be used for colinear fragments from each isolate despite loss or gain of restriction sites. The sizes of the viral DNAs varied slightly, but were all about 110 kbp. The zero point for the maps was taken as the start of the granulin gene which was orientated in the same direction as the polyhedrin gene on the map of multiple nucleocapsid nuclear polyhedrosis virus DNA. Differences between the four variants are discussed in relation to their insect host range.


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